Microflow Alfa ENG - Firmware 3.0.0 - Rev.5

5/21/2018 Microflow Alfa ENG - Firmware 3.0.0 - Rev.5

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AQUARIA MICROFLOW User Manual 12/08 rev.5 page 1

USER MANUAL

MICROBIOLOGICALAIR SAMPLER

Microflow 60

Microflow 90-C

Microflow 60/90-C


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Contents

INDEX page. 2

GENERAL INFORMATION page. 3Working principle page. 3

DESCRIPTION OF THE UNIT page. 4Case content page. 4Main features page. 4Technical data page. 5Back control panel page. 5

OPERATIVE INSTRUCTIONS page. 6Preparing the unit page. 6Placing the plate page. 6Setting a sampling program page. 7Function Sampling page. 7

Run a manual sampling page. 8Run a programmed sampling page. 9

Run a sequential sampling page. 11End of sampling page. 14

OTHER FUNCTIONS page. 15Battery level page. 16Setup date and time page. 17Stored samplings page. 17Download data page. 18Delete memory page. 19Flow rate calibration page. 20Setup language page. 20Setup head page. 21

Reset system page. 21

POWER SUPPLY page. 22

BATTERY CHARGER page. 22

UNIT DISINFECTION page. 24

TROUBLE SHOOTING page. 25

WARRANTY page. 26

CONVERSION TABLES page. 27

STANDARDS AND GUIDELINES page. 29

CERTIFICATES page. 30


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GENERAL INFORMATION

MICROFLOW has been developed for monitoring of air biocontamination level in critical places(e.g.: clean rooms, food plants, fermentations plants, etc.). The equipment conforms to the newEuropean ISO/TC 209 dated 25 July 1999 recommendation.MICROFLOW allows collection of a wide range of airborne biocontaminant by impacting on agarstandard plates (60 mm + 2 mm and 90 mm + 2 mm diameter, filled with every kind of culturemedia).MICROFLOW can be used successfully in a wide range of applications (air quality control inpharmaceutical, food, cosmetics plants, in hospitals, clinics and laboratories) for sterile or nonsterile areas monitoring. It is very useful also for air monitoring in building air conditioning (HVAC)plants.

Working principle

The air to be sampled is drawn through a perforated head, at constant velocity and for a period oftime depending on the condition of the area to be controlled. The inflow air impacts in the surface of

the culture media, chosen on the basis of the microbiological investigation to be carried out, placedin a 90 mm Petri dish. At the end of the sampling period the Petri dish is removed and placed into

an incubator. At the end of the incubation it will be possible to count the CFU/cm2 and evaluate theair biocontamination level of the critical area on the basis of air volume sampled .


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DESCRIPTION OF THE UNIT

Case content

MICROFLOW air sampler kit includes:

Carrying case Microbiological sampler "Microflow " 60 mm aluminium adding head (inside kit

Microflow 60 , cod. G.1004) 90-C mm aluminium adding head (inside kit

Microflow 90-C, cod. G.1015) 60 or 90 mm aluminium head assembly

(inside kit Microflow 60/90-C, G.1027) 1 pack of 10 PCA Rodac plates

(only inside kit Microflow 60 e 60/90-C, cod. G.1004 e G.1027)

Battery charger with full charge indicator

I.R. remote control Pencil torch 1 pack of 10 Contact plates (only Microflow 60 and 60-C and Microflow 60/90-C) User manual in English Calibration Report Warranty card

Main features

Used with: contact plates 60 + 2 mm (Microflow 60)Petri plates 90 + 2 mm (Microflow 90)

the same instrument allows to sample with 60 or 90 mm plates (Microflow 60/90-C) Rechargeable battery operated. Battery Ni/Mh (memory effect free) Silent electric fan microprocessor controlled to assure the maximum reliability of the air volume

sampled Compensation airflow system (models compensated) Low battery control and alarm User friendly. Prompts in 5 languages: English, French, German, Spanish, Italian. All functions

are visualised step-by-step. Delay start Total volume of sampled air programmable from 1 up to 2000 litres, with 1 litre steps Manual sampling (for air volumes greater than 2000 litres) Sequential sampling also available Storing and visualising up to 99 samplings, with record of progressive number, date, hour,

volume or time of sampling, flow rate, type of sampling (manual, sequential, programmable). USB port to download data to a PC (optional) Calibration control function (with optional calibration kit) Remote switch with infra-red rays control Two sampling positions (90-180) and built-in soc ket for tripod


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Technical data

Dimensions: 310 x 130 x 170 mm (W x D x H)Weight: 1,9 KgCase: Polyurethane, joint-free, easy to disinfect60 mm head: Autoclavable anodised aluminium head, with 219 holes ( 1 mm)90 mm head: Autoclavable anodised aluminium head, with 380 holes ( 1 mm)Plate support: Autoclavable anodised aluminium plate support for 60 and 90 mm 2 mm

plates.Flow rates: 30 60 90 - 100 (ISO/TC 209) -120 l/minSampled Air Volume: 1 - 2000 litres (and more with manual control)

Back control panel

- ON/OFF buttonThis is the power switch.Put in the ON position to power the unit.Place on OFF at the end of the use to save the battery charge.

- Data OutputUSB port (optional) to transfer on the PC the stored data

- Battery chargerconnector

- Remote controlinfra-red sensor

- DisplayHigh contrast graphic display matrix

- Start/Stop; Prog; Clear;Set; Enter; Up; Down.Programming multifunctional keys

Fig. 1 - Control panel


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OPERATIVE INSTRUCTIONS

Preparing the unit

Before to start the sampling, MICROFLOW must be disinfected, programmed, loaded with a plate

and put on a flat surface or on a suitable photographic tripod (avoid to screw too hard).

WARNING:dont screw too hard the tripod to avoid demage

MICROFLOW allows to operate with 60 + 2 mm or 90 mm + 2 mm AGAR PLATES. It dependson the head assembly chosen.

Before to operate its necessary to set the right plate diameter of the aluminium plate support. Thissetting will not be modified until the change of the type or brand of Petri dishes used for thesampling.

Placing the plate

Wear sterile gloves. Remove the sampling head handling with care to avoid any contamination ofits surfaces. Deposit the head on a clean surface.

Disinfect the aluminium plate support with a suitable disinfectant, avoiding liquid spillage inside theinstrument.

Place a sterile filled plate on the first aluminium platesupport. Press gently in outer direction and lay theplate on the others (see figure).Remove the dish lid,and deposit it on a clean surface.Close the sampling unit screwing clockwise thesterilised aluminium head on the plate support.

Aluminium platesupport

Screw

ScrewPlate holders


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Setting a sampling program

Switch on the unit by pressing ON push button.

On the display will appear sequentially the following start information:

FUNCTION: "Sampling"

Press [Start]key to start a sampling using the last stored parameters.

WARNING:using this procedure the delay start is not considered

Press [Prog]keyto enter a new sampling program.

Press [Up] and [Down]key to display the following indications :

When the needed sampling is shown, press [Enter].

MICROFLOW 90Version 3.0.0

01/05/09 12:00

Serial Number

PRG Program

SET Setup

START Start

Level 100%

Samplings Type:

Manual

change

ENTER select

Samplings Type:

Programmed

change

ENTER select

Samplings Type:

Sequential

change

ENTER select

COMPANY NAME

Address

Address

Address


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Run a Manual sampling

The operator can run a manual sampling with times or volumes different from the preset by themanual equipment control.

To program the Microflow on a manual sampling, display:

and Press [Enter]key.

It will appear:

Where xxx is the last selected flow rate.

If the flow rate is correct, press [Enter]. If not, select the needed flow rate using the [Up] and[Down] keys. When the flow rate needed is displayed press [Enter].

It will appear:

Where:Manual Samp.= manual sampling selectedxxx is the needed flow rate.

If the programmed data sampling are correct, press [Enter] and it will appear the sequence of thethree initial displays. If it is necessary to change some data, press the [Clear] key and the firstdisplay (Manual sampling) will appear.

Press [Start] key to start the sampling. The two red LED will switch on and, on the display, it willappear:

Where:t = sampling timeL= sampling volumeFr = selected flow rate

Press [Stop] key to end the sampling. The two red LED will switch off and, on the display, it willappear the following message:

A beep will continue until it will not be pressed [ENTER] to store the sampling data or [CLEAR] to

exit without storing .

Sampling type:

Manual

change

ENTER select

Flow Rate

xxx l/min

change

ENTER select

Manual Sampling

xxx l/min

ENTER select

Sampling end

Fr xxx t hh:mm:ss

ENTER esc

CLEAR delete

Manual sampling

t hh:mm:ss

L xxxx.x Fr xxx


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Run a programmed sampling

To program the Microflow on a programmed sampling, display:


It will appear:

xxxxis the last selected volume.

If the volume is correct, press [Enter]. If not, select the needed volume using the [Up] and [Down]keys, to increase or decrease the value, and the [Set]key to move the cursor.

WARNING:Programmed mode allows to sample air volume between 1 and 2000Lt. To sample greater air volumes it is necessary to work in Manual

mode.

When the volume needed is displayed press [Enter].

It will appear:

xxxis the last flow rate selected.


It will appear:

Set the needed delay using the [Up] and [Down] keys, to increase or decrease the value, and the[Set]key to move the cursor. When the delay needed is displayed press [Enter].

Sampling type:

Programmed

change

ENTER select

Volume

L xxxx change

ENTER select

Flow Rate

xxx l/min

change

ENTER select

Delay Start

00 : 00

change

ENTER select


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WARNING: Set EXACTLY delay time (hour an minutes) for the beginning of the

sampling.

If the delay start is not needed, leave 00:00

After the [Enter] key has been pressed, all the parameters will be displayed (Volume, Flow rate,Sampling time calculated on the volume and flow rate basis, and the eventual delay start)

Where:Fr = set flow rate (L/min.)V = sampling volume (Litres)Ds = delay startt = sampling duration (the sampling time calculationis based on the flow rate and volume set)

If the set data are correct, press [Enter] to save the program.The three first displays will appear.

If it is necessary to change one or more data, press the [Clear] key and it will be shown the initial

display with the programmed sampling settings. Press the [Enter] key up to the parameter that it isnecessary to change. Modify the parameter and press [Enter] to save the setting. Press [Enter]up to the summing up screen and save the set parameters pressing the [Enter] key.

The three initial displays will appear and the Microflow is ready for the sampling.Press [Start] key to start the sampling. The two red LED will switch on and, on the display, it willappear:

a) The delay start is not active:

The two red LED will brighten and, on the display, will be shown:

and then

At the end of the sampling period the red LED will go out and the following message willappear:

At the same time a beep will inform the user that the sampling is finished. A beep willcontinue until it will not be pressed [ENTER] to store the sampling data or [CLEAR] to exitwithout storing .

Fr xxx l/min

V yyyy L

Ds 00:00 t mm:ss

ENTER select

Program sampling

wait

start

Program sampling

t hh:mm:ss

L xxxx.x Fr xxx

Sampling endFr xxx L xxxx.x

ENTER esc

CLEAR delete


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b) The delay start is activated

The two red LED will lighten and, on the display, will be shown:

At the end of the delay start the LED will brighten and, on the display, will be shown:

and then

At the end of the sampling period the red LED will go out and the following messagewill appear.

At the same time a beep will inform the user that the sampling is terminated.The beep willcontinue until it will not be pressed [ENTER] to store the sampling data or [CLEAR] to exitwithout storing .

Run a sequential sampling

To program the Microflow on a sequential sampling, display:


It will appear:

xxxxis the last selected volume.

Wait please

Delay Start

hh : mm : ss

Sampling type:

Sequential

change

ENTER select

Volume

L xxxx

change

ENTER select

Program sampling

wait

start

Program sampling

t hh:mm:ss

L xxxx.x Fr xxx

Sampling end

Fr xxx L xxxx.x

ENTER esc

CLEAR delete


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If the volume is correct, press [Enter]. If not, select the needed volume using the [Up] and [Down]keys, to increase or decrease the value, and the [Set]key to move the cursor.

WARNING:The Seqential mode allows ONLY samplings with air volume between

1 and 2000 litres

When the needed volume is displayed press [Enter] key.

It will appear:

xxx is the last flow rate selected.


It will appear:

Where:

NUM SQ= number of sequential samplings

BreakSQ= break (minutes) between twosamplings

Set the needed data using the [Up] and [Down] keys, to increase or decrease the value, and the[Set]key to move the cursor.

WARNING:It is possible to set from 1 up to 99 cycles

It is possible to set a break between 1 and 120 minutes max.

When the sequence needed is displayed press [Enter].

It will appear:

Set the needed delay start for the first sampling using the [Up] and [Down] keys, to increase ordecrease the value, and the [Set] key to move the cursor. When the delay needed is displayedpress [Enter].

Flow Rate

xxx l/min

change

ENTER select

NUM SQ 01

BreakSQ 001 min

change

ENTER select

Delay Start

00 : 00

change

ENTER select


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WARNING: Set EXACTLY delay time (hour an minutes) for the beginning of the

sampling

If the delay start is not needed, leave 00:00

After the [Enter] key has been pressed, all the parameters will be displayed (Flow rate, volume, theeventual delay start, sampling time calculated on the volume and flow rate basis, number of setting

sequences, time between sequences).

Where:xxx = set flow rate (L/min.)yyyy = sampling volume (Litres)Ds 00:00 =Delay start

t mm:ss = sampling duration in seconds (the sampling time calculation is basedon the flow rate and volume set)

Nseq nn = number of sequencesBseq sss = break between a sampling and the following (seconds)

If the set data are correct, press [Enter] to save the program.The three first displays will appear.If it is necessary to change one or more data, press the [Clear] key and the it will be shown theinitial display with the programmed sampling settings. Press the [Enter] key up to the parameterthat it is necessary to change. Modify the parameter and press [Enter] to save the setting. Press[Enter] up to the summing up screen and save the set parameters pressing the [Enter] key.

The three initial displays will appear and the Microflow is ready for the sampling.

Press [Start]and the Microflow will start the sampling as shown for the programmed sampling.

It will appear:

During sampling:

t = countdown end sampling (h/m/s)L = sampling volume (Litres)Sq = sampling number respect the numbers

sampling set (es : 01/02 says that we aredoing the first sampling up two)

In this moment the LED will switch on.

Fr xxx l/min

V yyyy

Ds 00:00 t mm:ss

Nseq nn Bseq sss

Sequen. sampling

t hh:mm:ss

L xxxx.x Sq01/NN

Sequential

sampling

wait

start


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Between two sequences sampling:

Sq nn/NN = n sampling to do over the totalsampling to do (es : 02/02 says that weare waiting for beginning the secondsampling up two)

t = countdown end sampling (h/m/s)

In this moment the LED will brighten.

During the following sampling:

Only the number of sequences changes

In this moment the LED will switch on.

At the end of the sampling period the red LED will go out and then it will appear:

At the same time a beep will inform the user that the sampling is terminated. The beep will continueuntil it will not be pressed [ENTER] to store the sampling data or [CLEAR] to exit without storing .

End of sampling

Switch off the Microflow pressing the [Off] key.

Remove the sampling head unscrewing anticlockwise.

Close the plate with the lid, and after the identification, bring it to incubation.

Prepare the unit to the next sampling.

WARNING: In order to save energy when MICROFLOW is waiting for a new

sampling, after about 10 minutes of its inactivity the LCD display

lighting system automatically switch off, it will appear the indication

Stand by, until ENTER key is pressed.

Sequen. sampling

t hh:mm:ss

Next sampling

Sq nn/NN

Sequen. sampling

t hh:mm:ss

L xxxx.x Sq02/NN

Sampling end

Fr xxx Sq nn/NN

ENTER esc

CLEAR delete


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OTHER FUNCTIONS

Press [on/off] to switch on the instrument.

Press [Set] to enter in the setting menu of the MICROFLOW .

Press the [Up] key to scroll the following menus on the Display:

Press [Enter] to enter in a menu.

In theMicroflow 60/90-C (cod. G.1069), and ONLYin this instrument, after the display

It will appear:

Battery level

change

ENTER select

Setup

date and clock

change

ENTER select

Stored samplings

change

ENTER select

Delete memory

change

ENTER select

Flow rate

calibration change

ENTER select

Language Setup

change

ENTER select

Reset System

change

ENTER select

Setup

Language

change

ENTER select

Setup head

change

ENTER select


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When inside the instrument is fitted the download data system (cod. G1117), after the display

It will appear:

FUNCTION: "Battery level"

In the battery level menu:

Press [Enter] and it will appear while the fun switch on:

xxx % battery level

000% means that the instrument isnt underrecharge

The battery level is shown by a number (%) on by the wording HIGH, WARNING and LOW .

The word WARNING will appear when the level is around 40%, in this case we suggest to rechargethe battery because the instrument will be able to sample for about 20 minutes, lower is thepercentage and lower is the possible working time .The word LOW will appear when the level arrives to 5%, in this case you have to recharge thebattery before use the instrument, you can only press the [ON/OFF] key.

AQUARIA RECOMMEND TO CHARGE THE BATTERY WHEN ITARRIVES IN WARNING LEVEL.

Press the [Clear]key to exit from the Battery level menu.

Press again the [Clear]key to visualize the first three displays.

Downloaded data

change

ENTER select

Level HIGH

xxx%

Charge current

000%

Stored samplings

change

ENTER select

Battery Level

change

ENTER select


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FUNCTION: "Setup date and clock"

In the Setup date and clock menu,

Press [Enter] after the visualisation and it will appear:

Set data and time using the [Up] and [Down] keys, to increase or decrease the value, and the[Set]key to move the cursor.

Press the [Clear]key to exit from the Setup date and clock menu.Press one time the [Clear]key to stay in the Set menu. Press two times the [Clear]key to visualizethe first three displays.

FUNCTION: "Stored samplings"In the Stored samplingsmenu:

Press [Enter] after the visualisation an it will appear:

a) Manual sampling

Nxxx = sampling sequential numberdd/mm/yy = sampling date (day/month/year)hh:mm = sampling time (hour/minutes)Fr = yyy = flow rate (L/min)H = sampling headt= wwww = sampling duration (seconds)

Data 25/02/07

Hour 12:42:03

change

ENTER select

Setup

date and clock change

ENTER select

Stored sampling

change

ENTER select

N xxx

dd/mm/yy hh:mm

Fryyy l/min H 90

t wwww sec


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b) Programmed sampling

Nxxx = sampling sequential numberdd/mm/yy = sampling date (day/month/year)hh:mm = sampling time (hour/minutes)Fr = yyy = flow rate (L/min)

H = sampling headV=zzzz = air volume sampled (Litres)

c) Sequential sampling

Where:Nxxx = number of samplingdd/mm/yy = sampling date (day/month/year) of thh:mm = sampling time (hour/minutes)Seq = sequential samplingFr=yyy = flow rate (L/min)H = sampling head

V=zzzz = air volume sampled (Litres)01 of 02 = the first sampling of a sequence of 2samplings

02 of 02 = the second sampling of a sequenceof 2 samplings

IS xxx =delay start between sequences ofsampling

Press the [Clear]key to exit from the Stored samplings menu.

Press one time the [Clear)] key to stay in the Set menu. Press two times the [Clear] key tovisualise the first two displays.

FUNCTION: "Download data "(only for Microflow with PC interface installed)

In the Download datamenu:

Tx = numbers of samplings to download


After the download it will appear automatically the set menu. Press the [Clear]key to visualise thefirst three displays.

N xxx Seq 01/02

dd/mm/yy hh:mm

Fryyy l/min H 90

V zzzz l IS xxx

N xxx

dd/mm/yy hh:mm

Fryyy l/min H 90

V zzzz l

Download Data

change

ENTER select

Wait please

Download data

Tx 002/002

Downloaded Data


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NOTE:during this step, the Microflow has to be connected to a PC with the proper cable. Wesuggest to download only after the connection with the PC.

WARNING: Every time the download is done, the Microflow transfer all the data to the

PC. We suggest to erase the data stored on the Microflow after the

download.

FUNCTION: "Delete memory"In the Delete memorymenu


Select the starting point of deletion of samplings memorizedPress the [Up] and [Down] keys to scroll the sampling number.Use [Set] to move the cursor to select the end point of deletion of samplings memorizedPress the [Up] and [Down] keys to scroll the sampling number.


If it is not necessary to delete the stored data press the [Enter]key to exit from this menu.If it is necessary to delete the data press the [Down]key and it will appear:

Press the [Enter] key to delete all the stored data an it will appear

After waiting the time for the memory reset, it will appear automatically the set menu. Press the[Clear]key to visualise the first three displays.

Delete memory

change

ENTER select

Are you sure ?

No

Are you sure ?

Yes

Erase

memory

Deletesamplings

in memory

from 001 to 001

Deletesamplings

in memory

from 001 to 001


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FUNCTION: "Flow rate calibration"

In the Flow rate calibrationmenu:

Press [Enter] after the visualisation and It will appear:

In order to avoid an undesired cancellation of the calibration, the passwordwill be supplied ONLY at the customers who buy the MICROCONTROL IIcalibration kit.Press the [Enter]key to exit from the Flow rate calibration menu.

Press one time the [Enter]key to stay in the Set menu. Press the [Clear]key to visualise the firstthree displays.

FUNCTION: " Language Setup "

In the Language Setupmenu:

Press [Enter] after the visualisation and It will appear:

Press the [Up] and [Down] keys to scroll the available languages (ITALIANO, ENGLISH,FRANCAIS, DEUTSCH, ESPANOL).

Press [Enter] to confirm.After the confirmation, it will appear automatically the set menu. Press the [Clear]key to visualisethe first three displays.

Password

0000

Flow rate

calibration

change

ENTER select

Language Setup

ENGLISH

Language Setup

change

ENTER select


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FUNCTION: "Setup head"(only for Microflow 60/90-C)

In the Setup headmenu:


Press the [Up] and [Down] keys to scroll the available heads: 60 and 90.Press [Enter]and the following display will appear:

Press the [Up]and [Down]keys to choose yes or no and confirm with [Enter]key.

The Setup head menu will be displayed again. Press the [Clear]key to come back to the mainmenu.

FUNCTION: "Reset system"

In the reset systemmenu:


This menu can be only used by the manufacturer.

Press two times the [Enter]key to visualise the first three displays.

Password

0000

Reset System

change

ENTER select

Setup head

change

ENTER select

Head

90

change

ENTER select

Are you sure ?

No


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POWER SUPPLY

The unit is powered by a 8 elements Ni/MH battery pack (each element: 1,2V) protect againstshort-circuits and termic overcharges.

NOTE: These batteries have no memory effect and can be recharged at any time withoutdamaging the elements

The chip installed on the instrument avoid to run a sampling if the battery level is not enough tomaintain a constant flow rate as long as necessary

BATTERY CHARGER

Discharge battery

a) Sampler switch on but not operative

If the MICROFLOW has been left switched on, although not operating, the battery level will slowlydecrease. If the level decrease over the limit WARNING, will appear:

b) Sampler operativeIf MICROFLOW is sampling and during the sampling the battery charge decreases under theWARNING level (and it is not possible to maintain the constant flow rate), the display will show:

Fr: Flowrate settedT: Past sampling time

Push the button [ Enter ] the sampling will be storedPush the button [ Clear ]the sampling will not be stored

ATTENTION : The sampling will not be stored on the basis of the values setted but on the basis ofthe real sampling time

After pressing the button [ Enter / Clear ]will appear :

and its not possible proceed with other samplings until the instrument will not be charge.

Battery

Level

Very low

WARNING

Battery

Level

Very low

WARNING

Sampling end

Fr xxx t hh:mm:ss

ENTER

CLEAR


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Battery charge

ATTENTION : to avoid any damages to batteries or power adapter we suggest to follow thefollowing sequence:

- Connect recharger jack to Microflow

- Connect the charger the power supply

After the recharge, disconnect the charger to the power supply then disconnect the jack from theinstrument.

The complete recharging time is about 4-5 hours but it depends on the residual battery charge.

ATTENTION : we recommend to recharge the batteries when the instrument is off.You can also recharge the instrument switched on but, in this case the charging time could belonger and the full battery recharge is not guaranteed.

If the Microflow remains connected to the power for more than 8 hours, the charger automaticallyswitches off.

- Instrument OFF

The charging of the instrument may be followed by observing the LED on the instrument

LED flash rapid: the instrument is in recharge

LED flash slow: the instrument is fully charged (battery level 100%) and in maintenance phase

LED off: the instrument is fully charged (battery level 100%)

- Instrument ON

The LED will be switched off if the instrument is switched on but not active and will display the word"Battery charging"

ATTENTION : If necessary the Microflow can also be used connected to the power supplythrough the battery charger

During the recharging, you can enter the menu and select "level battery charge", on the display willappear both the charge percentage and the current recharge percentage.If the charger is connected to the instrument, the battery charge level showed on the display will be100% (this means that the battery is charging at 100%) while the current recharge will be 100% atthe beginning but it will decrease during the battery charge.

If the charger is not connected to the power supply the current recharge will be equal to 0% (anycurrent recharge is issued) and the battery charge real percentage value will be displayed.

ATTENTION : To avoid the total batteries discharge, under the threshold value, that wouldcause a very long recharge battery time (also over 10 hours), we recommend to recharge thebatteries at least once a month even if the instrument has not been used and in any case, torecharge always the instrument after the utilisation .

Replacement batteriesTo change the batteries is enough to unscrew the metal panel placed at the bottom of theinstrument.Be careful to avoid lifting the lid to detach the lead from the IR sensor (if something happensreconnect the cable paying attention to the color of wires).

The batteries are joined to the battery holder by a velcro strip : remove the batteries, disconnect thebattery connector and replaced the batteries.


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Head Control - valve

UNIT DISINFECTION

MICROFLOW must be cleaned and disinfected after each sampling cycle to avoid crosscontamination which could modify the reliability of the sampling.

Switch off the unit.

Clean the surface of MICROFLOW using a sterile cloth wet with a suitable disinfectant. Afterdrying, the unit is ready for a new sampling cycle.

WARNING: DONT SPRAY disinfectant solution directly on the instrument or the

keyboard: these solutions could enter inside the instrument and they

cause damage as example and oxidation of the circuits.

AQUARIA WILL NOT RECOGNIZE THE WARRANTY CAUSED OF

UNSUITABLE DISINFECTANT SOLUTION

The sampling head and the support plate can be sterilized in autoclave, following the standardprocedure of sterilisation ( i.e. 120 C, 18 minute s).

Between two samplings is necessary to disinfect the sampling head with pencil torch supplied withthe starting kit. In the absence of this, it is possible to use a sterile cloth with disinfectant over this. Itis very important to avoid the liquid penetration inside the instrument.

Let dry at room temperature. After drying the sampling head is ready to be used.

Sampling head fire disinfecting

The MICROFLOW kit is equipped with a pencil to disinfect the head. Disinfect always the head

between two samplings to avoid a cross-contamination.

To switch on the pencil torch rotate clockwise the control-valve. When the gas goes outfrom the head, bring the fire to light the pencil.

WARNING: Pass quickly the fire on the interior part of the sampling head.

Do not stop the fire on a specific point of the head, it can be damaged.

To switch off the pencil torch rotate counter-clockwise the control-valve.

To recharge the pencil torch it is possible to use the Butan gas used for cigarette-lighters.

Before to recharge the pencil torch be sure it is switched off. The complete recharge needs about10 seconds. It is necessary to wait some minutes so that the gas is stable.

For correct recharging it is recommended to keep horizontal the pencil torch and the and therecharge cartridge

P E N C I L T O R C H


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MAINTENANCE

All operations shall be performed by qualified personnel only!

There are no parts replaceable by the user himself. In case of problems please call your dealer forservice.

WARNING: It is recommended to calibrate the instrument at least once ayear

TROUBLE SHOOTING

In the unit nothing is switched on Verify:

1. The unit is switch on

2. The battery is charged

3. If doesnt work again, please call for service.

The blower motor does not work Please call for service

The infrared remote control switch doesntstart the sampling

Verify:

1. Infrared remote switch battery is charged

2. If doesnt work again, please call for service.

LCD display doesnt work properly Please call for service.

LCD display shows question marks or othercharacters

Please call for service.

Red LED doesnt light during the sampling Please call for service.

Blower motor make noise Verify:

1. Presence of foreign bodies inside the fan

2. If noise still persists, please call for service

Fan velocity is out of any control 1. If the problem is present for a flow rate only,calibrate that flow rate with the calibration kitfollowing related instructions.

2. If the problem is present for all flow rates,verify each flow rate calibration

3. If the model is 60/90, take off the head andthe support plate. Verify the alignment of thefan

4. If the problem still persists, please call forservice.

Soft touch control panel doesnt work. Please call for service.


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WARRANTY

AQUARIA provides 1 year warranty on the instrument starting on the date of purchase.

Within the warranty period Aquaria will eliminate, free of charge, any defects in the MICROFLOW

microbiological air sampler resulting from failures in material or workmanship either by repairing, orexchanging parts or exchanging the whole appliance as Aquaria may choose.

Damages caused by improper use as well as negligence to use the instrument, normaldeterioration of internal parts, some defects with negligible effects on the ordinary function of theinstrument are not included in this warranty.

This warranty declines in case of technical intervention repairs which are carried out by nonauthorized personnel.

Aquaria is not ever responsible, in front of the purchaser or end user, for damage caused by peopleor things (included the same instrument) in relation to the functioning of the instrument, that it iscaused by:

a) any type of work or modification carried out inside the instrument unit, or its accessories,without Aquaria previous authorization and /or Aquaria technical direct instructions, even inthe case that such works are carried out by Aquaria employees, but without Aquaria properauthorization.

b) to use the MICROFLOW and/or its accessories for purposes which are different from theoriginal purposes indicated by Aquaria at the time of planning the design, manufacture andsell the instrument

c) to clean MICROFLOW and/or its accessories with chemical products different from theAquaria indications and /or to use Microflow under different operational conditions asindicated by Aquaria

For further information concerning Microflow please contact:

For technical assistance please contact:

AQUARIA srl Via della Levata, 1420084 - Lacchiarella, Milan (Italy)

Tel +39-02-90091399 - FAX +39-02-9054861E-mail: [email protected]

AQUARIA srl- Via della Levata, 1420084 - Lacchiarella, Milan (Italy)

Tel +39-02-90091399- FAX +39-02-9054861

E-mail: [email protected]


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CONVERSION TABLES

To correlate the CFUs (Colony Forming Units) present on the agar plate to the most probablenumber (MPN) of micro-organisms per cubic meter of air sampled, the following formula is used:

Pr = N [1/N + 1/(N-1) + 1/(N-2) + ... + 1/N-(r+1)]

Where:Pr = most probable number of micro-organisms in the volume of air sampledN = number of holes on Microflow sampling headR = number of CFUs on the agar plates after incubation

How to calculate a result:

To calculate the correct result, following data are necessary:- Chosen sampling volume on Microflow (litres)- Number o colonies on the agar plate after incubation (cfu)- Corrected number of colonies from tables below (cfu)

Number of cfu/m3 = Corrected number of colonies from tables below (cfu) x 1000

Chosen sampling volume on Microflow (litres)

CONVERSION TABLE Microflow 60

r Pr r P

r r P

r r P

r r P

r r P

r r P

r r P

r r P

r

1 1 27 29 53 61 79 98 105 143 131 199 157 275 183 393 209 666

2 2 28 30 54 62 80 99 106 144 132 201 158 279 184 399 210 688

3 3 29 31 55 63 81 101 107 146 133 204 159 282 185 405 211 712

4 4 30 32 56 65 82 102 108 148 134 206 160 286 186 412 212 739

5 5 31 33 57 66 83 104 109 150 135 209 161 290 187 418 213 771

6 6 32 35 58 67 84 106 110 152 136 212 162 293 188 425 214 807

7 7 33 36 59 69 85 107 111 154 137 214 163 297 189 432 215 851

8 8 34 37 60 70 86 109 112 156 138 217 164 301 190 440 216 906

9 9 35 38 61 71 87 111 113 158 139 220 165 305 191 447 217 979

10 10 36 39 62 73 88 112 114 160 140 222 166 309 192 455 218 1.088

11 11 37 40 63 74 89 114 115 163 141 225 167 313 193 463 219 1.307

12 12 38 42 64 75 90 116 116 165 142 228 168 317 194 471

13 13 39 43 65 77 91 117 117 167 143 231 169 322 195 480

14 14 40 44 66 78 92 119 118 169 144 234 170 326 196 489

15 16 41 45 67 80 93 121 119 171 145 237 171 331 197 499

16 17 42 47 68 81 94 122 120 173 146 240 172 335 198 509

17 18 43 48 69 83 95 124 121 175 147 243 173 340 199 519

18 19 44 49 70 84 96 126 122 178 148 246 174 345 200 530

19 20 45 50 71 86 97 128 123 180 149 249 175 349 201 542

20 21 46 52 72 87 98 130 124 182 150 252 176 354 202 554

21 22 47 53 73 89 99 131 125 185 151 255 177 360 203 567

22 23 48 54 74 90 100 133 126 187 152 258 178 365 204 580

23 24 49 55 75 92 101 135 127 189 153 261 179 370 205 595

24 25 50 57 76 93 102 102 128 192 154 265 180 376 206 611

25 26 51 58 77 95 103 139 129 194 155 268 181 381 207 628

26 28 52 59 78 96 104 141 130 196 156 272 182 387 208 646


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CONVERSION TABLE Microflow 90

r Pr r P

r r P

r r P

r r P

r r P

r r P

r r P

r r P

r


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STANDARDS AND GUIDELINES

Standards and guidelines (pharmaceutical industry):

Cleanroom classification acc. To EU GMP guidelines (for sterile products) valid from January 1,

1997

CleanroomClass

Max. Number Of Particles/m = 0,5 m Max. Number Of Particles/m = 5 m

A 3.500 0B 350.000 2.000C 3.500.000 20.000D - -

Cleanroom classification acc. To Federal Standard 209E (United States); USP

Cleanroom

Class

Max. Number Of Particles/m = 0,5 m Max. Number Of Particles/m = 5 m

100 3.530 -10.000 353.000 2.470100.000 3.530.000 24.700

Microbial limits:

Microbial classification acc. To EU GMP guidelines (for sterile products) valid from January 1,1997

CleanroomClass

cfu/m air Petri plate ( 90 mm);cfu / 4 h

Rodac plate ( 55 mm);cfu / 4 h

Finger print;5 finger cfu

A < 1 < 1 < 1 < 1B 10 5 5 5C 100 50 25D 200 100 50 -

Acc. To draft USP chapter

CleanroomClass

cfu/m air Surface cfu /24 cm

2Personell, glovescfu / 24 cm

2

Personell, masks, cap,overall cfu / 24 cm

2

100 < 3 3 < 1 < 110.000 < 20 5

10 (floor)20 10

100.000 < 100 - - -

Test frequencies (air sampling) for environmental monitoring:

Acc. To USP chapter 2nd

. Draft (March/April 1995)

Aseptic production (cleanroom area) Evaluation frequencyClass 100 Every shiftClass 10.000 DailyClass 100.000 2 times per weekClass 100.000(non-product / container contact)

1 time per week


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The Company

AQUARIA srl.Via della Levata, 14 20084 Lacchiarella (MI) Italy

nella persona del suo Presidente e Legale rappresentantein the person of President and Legal representative

Emilio Dadati

DICHIARA CHEdeclares that

il campionatore daria microbiologico, mod. MICROFLOW

the MICROFLOW microbiological air samplerMatricola N.

conforme alle seguenti normative Europee:conforms to the following E.C. standard

Immunit: IEC 1000 4- 3 (R.F.E.M. Field)Immunity: IEC 1000 4 2 (Electrostatic Discharge)

Emissione: EN 55011 (Conducted Emission)Emission: EN 55011 (Radiated Emission Pre-Compl.)

Altre: CEI EN 50082 - 2 (Generic Immunity Std)Others: CEI EN 50081 - 1 (Generic Emission Std)

Lacchiarella, .

AQUARIA srl.

The President


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Microflow Alfa ENG - Firmware 3.0.0 - Rev.5

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Transcript of Microflow Alfa ENG - Firmware 3.0.0 - Rev.5