VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome...

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Erwin R. Schmidt Institut für Molekulargenetik Vorlesung #4 07. 05. 2013 VL Einführung in die Gentechnologie

Transcript of VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome...

Page 1: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm

Erwin R. Schmidt Institut für Molekulargenetik

Vorlesung #4 07. 05. 2013

VL Einführung in die Gentechnologie

Page 2: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
Page 3: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm

Substitutionsvektoren

Linker Arm „Stuffer“ Rechter Arm

Integrat

Ligation

Page 4: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
Page 5: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
Page 6: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm

Sal Bam Eco Eco Bam Sal

Verdau mit Sal I + Eco

Sal Bam Eco Eco Bam Sal

Sal

Sal

Eco

Eco

Sal

Sal

Fällen mit Isopropanol

Eco

Eco

Sal

Sal

Integrat

Linker Arm Rechter Arm „Stuffer“

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Lambda Zap: Moderne Vektoren Mit Phagen und Plasmid- eigenschaften

Page 8: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
Page 9: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
Page 10: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
Page 11: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
Page 12: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
Page 13: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
Page 14: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
Page 15: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
Page 16: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
Page 17: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
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Page 21: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
Page 22: VL Einführung in die Gentechnologie - uni-mainz.de€¦ · Vektor: PAC: PI artificial chromosome Short Arm Ad.2 Stuffer Insert DNA PI and Plasmid Repiicons; ka.'{, Ligate Long Arm
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P-Elemente sind Transposons bei D. melanogaster, die eine Keimbahn-spezifisch aktive Transposase exprimieren