Update on Senecavirus A

Senecavirus A = Seneca Valley Virus

Acknowledgements• Dr. Pablo Pineyro• Dr. KJ Yoon• Dr. Darin Madson• Dr. Daniel Linares• Dr. Rachel Derscheid• Dr. Kent Schwartz• Dr. Rodger Main• Dr. Pat Halbur

• Dr. Derald Holtkamp• Dr. Paisley Canning• Dr. Jessica Bates• Dr. Abbey Canon• Dr. Dave Baum• Dr. JQ Zhang• Dr. Phil Gauger• Dr. Karen Harmon

Dr. Paul Sundberg

Seneca Virus A (Seneca Valley Virus)• Senecavirus A is a non-enveloped single-stranded RNA

virus of the family Picronaviradae. • Foot and Mouth Disease Virus (FMDV) and swine

vesicular disease virus (SVDV) is also a member of this same viral family.

• Clinical signs are INDISTINGUISHABLE from any of the 4 Foreign Animal Swine Vesicular Diseases.

Seneca Virus A (Seneca Valley Virus)• Disease has been reported in the United States,

Canada, Australia, Italy, New Zealand and most recently in Brazil (2014).

• First case described in 1988 (cell culture contaminant)– NOT A NEW DISEASE– Typical for APHIS to investigate 2-4 cases per year

• Idiopathic Vesicular Disease Senecavirus A

Is Senecavirus A really causing Idiopathic Vesicular Disease?

• Up until recently, Koch’s postulates have NOT been fulfilled for Senecavirus A and Idiopathic Vesicular Disease.

• Joint study between USDA and ISU have completed this in October.– Established clinical signs in 9 week old pigs and breeding age

gilts both 2-4 days after inoculation.Drs. Lager, Montiel, Buckley – USDA ARS

Case Reports: Growing Pigs

Case: Commercial Finishing Herd• 1200 head finishing barn with report of acute lameness and

vesicular lesions on snout and feet.– 20-30% prevalence initially (overnight)– Market weight animals

• Vet reported to state veterinarian, FAD investigation conducted – Negative for vesicular diseases– Positive for Seneca Valley Virus (both ISU and FADDL)

Case 2: Commercial Finishing Herd - July 15th• First cut of pigs to go to market was July 10th

– Dirty market truck– Commercial loading crew

• First clinical signs reported on July 15• Over next 10-14 days

– 80-90% of pigs had some sort of lesions• Vesicular lesions or lameness• “Pigs crawling to the feeders”

Deep nail bed hemorrhages

A B C A B C A B C A B C A B C D

0

10

20

30

40

Ct V

alue

s

Vesicular fl

uid

Swab

Nose scraping

Serum

Oral Fluid

Commercial Finishing herd, 7/29/15 – 14 days into outbreak

Oral FluidCT 18-22

Oral Fluid is an excellent sample for detection of Senecavirus A in populations

PCR CT Values:The lower the number, the

more virus that there is in the

sample

Oral Fluids – 4 weeks into break

North 6 North 1 South 5 North 9 South 1 North 10 South 6 North 4 North 5 North 2 North 3 North 7 North 8 South 3 South 4 South 2 South 7 South 9 South 80.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

27.225.5

27.328.3

25.7

28.7 28.2 28.726.6

27.626.3

29.227.6

23.4

26.728.4

27.128.6

26.2

Oral Fluid CT - 4 weeks post break

Oral fluids can detect the virus for a long time!

Finishing Case• Allowed 30 days for pigs to heal all the lesions and

lameness subside.• Sold all the remaining pigs without incidence.

– Good communication with Packer and FSIS• Second finishing case

– Only 10-15% of pigs affected– Sold them 2 weeks later.

Case Report: Senecavirus A in Sow Farms

Seneca Virus Breeding Herd Cases• Week of August 17th

– Reports of high neonatal morbidity and mortality in pigs less than 7 days.

• With or without diarrhea (more common with diarrhea)• Not usually finding much for other diarrhea agents

– Some sows with high fevers early on (104-106oF)– Some sows not eating fully (not much for off feed)– Mortality is short lived (4-7 days)

Similar to reports from Brazil in 2014-2015

Seneca Virus Breeding Herd Cases• With descriptions similar to these, pathologists began

to run SVV PCR on various samples from submitted cases started finding positives!!

• Upon further investigation, then finding evidence of vesicular disease in breeding age animals.– 10-40% prevalence

• Vesicles or coronary band lesions– NO LESIONS IN PIGLETS, Gross or Histologically.

Breeding Herd Lesions• Lesions in sows in farrowing rooms

– Affected litters• Sows had a few vesicles on the nose• Lots of lesions on the feet

– Coronary band ulcerations– Interdigital ulcers– Deep nail hemorrhages

• Saw similar lesions in unaffected litters, just not as severe.– No lesions in the pigs at all

Senecavirus A in neonatal pigs

Senecavirus A in neonatal pigs

Ruptured Vesicle

SVV PCR on neonatal pigs (less than 7 days)

A1 serum A2 serum A3 serum A4 serum B1 serum B2 serum B3 serum B4 serum Feces A Feces B Skin Coronary band0

5

10

15

20

25

30

18

24.2

21.6

16.5

22.9

27

20.5 20.4

24.6

20.1 20.89

25.22

Seneca Valley Virus PCR testing on neonatal piglets (less than 7 days of age)

Lots of virus, but NO DISTINCT HISTOLOGIC LESIONS

What’s been the impact on production?• Small increase (2-5%) in % PWM

for 1 week in most cases.• May double % PWM if there are

other agents present– Clostridium difficile– Rota virus

• No reports of reproductive impact (Conc. Rate, Farrow Rate, Litter Size)

Current Status of Senecavirus A at ISU VDL7/

26/2

015

8/2/

2015

8/9/

2015

8/16

/201

58/

23/2

015

8/30

/201

59/

6/20

159/

13/2

015

9/20

/201

59/

27/2

015

10/4

/201

510

/11/

2015

10/1

8/20

1510

/25/

2015

11/1

/201

511

/8/2

015

11/1

5/20

1511

/22/

2015

11/2

9/20

1512

/6/2

015

12/1

3/20

1512

/20/

2015

12/2

7/20

151/

3/20

161/

10/2

016

1/17

/201

6

02

4

68

10

12

14

Senecavirus A PCR Positive Cases by Farm Type

Sow Growing Pig Unknown

Date by Week

# SV

A PC

R Po

sitiv

e Ca

ses

Where have we seen SVA at the ISU VDL?

)

Jianqiang Zhang, et al

New isolates appear to be very different than the older ones and similar to Brazilian isolates

What should you do if you suspect SVA??• If you see suspect lesions on nose and/or coronary bands?

– Contact your veterinarian and State/Federal Officials– They will determine the next course of actions

• DO NOT attempt to sell pigs with active lesions.– Wait until they are completely resolved.

• See an increase in % PWM in neonatal pigs (less than 7 days)– Look for vesicular lesions contact State/Federal Officials

Summary• Seen a significant increase in cases of Idiopathic Vesicular

Disease– Senecavirus A in all cases

• Senecavirus A in cases of increases in % PWM in neonatal pigs– Short duration (4-7 days)

• Clinical pictures match description of cases in Brazil over the past year.

• The virus may have changed from historical isolates.– This may explain the increase in the number of cases

Questions??