Appendix

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SYMPOSIUM PARTICIPANTS

Daniel Abramowicz, General Electric Company, P. O. Box 8, Schenectady, New York 12301, U.S.A.

Angelo Azzi, Institute of Biochemistry and Molecular Biology, University of Bern, Bern, Switzerland

Rajeev Bawa, Department of Biology, Rensselaer Polytechnic Institute, Troy, New York 12180-3590, U.S.A.

Diana S. Beattie, Department of Biochemistry, School of Medicine, West Virginia University, Morgantown, West Virginia 26506, U.S.A.

Andrew Beavis, Department of Pharmacology, Medical College of Ohio, Toledo, Ohio 43699, U.S.A.

Jan A. Berden, Laboratory of Biochemistry, University of Amsterdam, Amsterdam, The Netherlands

Eric Block, Department of Chemistry, State University of New York at Albany, Albany, New York 12222, U.S.A.

Maurizio Brunori, Institute of Biochemistry, Faculty of Medicine, University of Rome, Rome, Italy

Maria Luisa Campo, Department of Biochemistry, Molecular Biology and Genetics, University of Extremadura, Caceres, Spain

Ernesto Carafoli, Laboratory of Biochemistry, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland

Samuel H. P. Chan, Department of Biology, Syracuse University, Syracuse, New York 13210, U.S.A.

Sunney I. Chan, Department of Chemistry, California Institute of Technology, Pasadena, California, U.S.A.

Britton Chance, Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, U.S.A.

Brad N. Chazotte, Department of Anatomy, School of Medicine, University of North Carolina, Chapel HilI, North Carolina 27514, U.S.A.

591

592 Symposium Participants

John Y. L. Chiang, Department of Biochemistry, College of Medicine, Northeastern Ohio University, Rootstown, Ohio 44272, U.S.A.

John Cobley, Department of Chemistry, University of San Francisco, San Francisco, California 94117-1080, U.S.A.

Stephen P. Cramer, SChlumberger-Doll Research, Old Quarry Road, Ridgefield, Connecticut 06877-4108, U.S.A.

Daniel DerVartanian, Department of Biochemistry, University of Georgia, Athens, Georgia 30602, U.S.A.

Simon de Vries, Laboratory of Biochemistry, B.C.P. Jansen Institute, University of Amsterdam, Amsterdam, The Netherlands

Joyce Diwan, Department of Biology, Rensselaer Polytechnic Institute, Troy, New York 12180-3590, U.S.A.

P. Leslie Dutton, Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, U.S.A.

Henry Ehrlich, Department of Biology, Rensselaer Polytechnic Institute, Troy, New York 12180-3590, U.S.A.

Shelagh Ferguson-Miller, Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824, U.S.A.

Karl Folkers, Institute for Biomedical Research, The University of Texas at Austin, Austin, Texas 78712, U.S.A

Joachim Frank, Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, New York 12201, U.S.A.

Keith Garlid, Department of Pharmacology, Medical College of Ohio. ToledO, OH 43699, U.S.A.

Graham N. George, Exxon Research and Engineering, Annandale, New Jersey 08801, U.S.A.

Laurie Graham, Department of Biology, Rensselaer Polytechnic Institute, Troy, New York 12180-3590, U.S.A.

Sharmila Gupte, Department of Anatomy, School of Medicine, University of North Carolina, Chapel HilI, North Carolina 27514, U.S.A.

Charles R. Hackenbrock, Department of Anatomy, School of Medicine, University of North Carolina, Chapel HilI, North Carolina 27514, U.S.A.

Teresa Haley, Department of Biology, Renssealer Polytechnic Institute, Troy, New York 12180-3590, U.S.A.

Peter Hinkle, Department of Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, U.S.A.

Samuel Ho, Laboratory of Bioenergetics, State University of New York at Albany, Albany, New York 12222, U.S.A.

Symposium Participants 593

Andre T. Jagendorf, Division of Biological Sciences, Plant Science Building, Cornell University, Ithaca, New York 14853-5908, U.S.A.

John R. Kettman, Jr., Department of Microbiology, University of Texas Health Science Center, Dallas, Texas 75235-9048, U.S.A.

Chong H. Kim, Laboratory of Bioenergetics and Department of Biological Sciences, State University of New York at Albany, Albany, New York 12222, U.S.A.

Tsoo E. King, Laboratory of Bioenergetics and Department of Chemistry, State University of New York at Albany, Albany, New York, 12222, U.S.A.

Martin Klingenberg , Department of Biochemistry, Uni versity of Munich Munchen 2, Federal Republic of Germany

Jeffrey Kramer, Department of Biology, Rensselaer POlytechnic Institute, Troy, New York 12180-3590, U.S.A.

Jack Lancaster, Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322, U.S.A.

C. P. Lee, Department of Biochemistry, School of Medicine, Wayne State University, Detroit, Michigan 48201, U.S.A.

Georgio Lenaz, Department of Biochemistry and Biology, University of Bologna, Bologna, Italy

Yun Li, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, U.S.A.

School of Medicine, Pennsylvania 19104,

Russel LoBrutto, Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, pennsylvania 19104, U.S.A.

Bruce Mackler, Pediatrics Department, University of Washington, Seattle, Washington 98195, U.S.A.

Carmen Mannella, Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, New York 12201, U.S.A.

Raphael Mannino, Department of Microbiology and Immunology, Albany Medical College, Albany, New York 12208, U.S.A.

Emanuel Margoliash, Department of Biochemistry, Northwestern University, Evanston, Illinois 60201, U.S.A.

Vincent Massey, Department of Biological Chemistry, School of Medicine, The Univeristy of Michigan, Ann Arbor, Michigan 48109-0606, U.S.A.

Hiroshi Matsubara, Department of Biology, Faculty of Science, Osaka University Osaka 560, Japan

James W. McGill, Department of Biology, Rensselaer Polytechnic Institute, Troy, New York 12180-3590, U.S.A.

594 Symposium Participants

Peter Mitchell, Glynn Research Institute, Boclrnin, Cornwall PL30 4AU, England

Mohammed Mustafa, UCLA School of Public Health, Los Angeles, California 90024, U.S.A.

Yash Myer, Departrnent of Chernistry, State University of New York at Albany, Albany, New York 12222

Peter Nicholls, Departrnent of Biological Sciences, Brock University, St. Catherines, Ontario Canada

Tornoko Ohnishi, Departrnent of Biochernistry Medicine, University of Pennsylvania, 19104, U.S.A.

and Biophysics, School of Philadelphia, Pennsylvania

Yutaka Orii, Departrnent of Biology, Faculty of Medicine, Kyoto University, Kyoto 606, Japan

Nanette Orrne-Johnson, Departrnent of Biochernistry and Pharrnacology, School of Medicine, Tufts University, Boston, Massachusetts 02111, U.S.A.

Takayuki Ozawa, Departrnent of Biornedical Chernistry, Faculty of Medicine, Nagoya University, Nagoya 466, Japan

Sergio Papa, Institute of Biological Chernistry, Faculty of Medicine, University of Bari, Bari, Italy

Robert Parsons, Departrnent of Biology, Rensselaer Polytechnic Institute, Troy, New York 12180-3590, U.S.A.

Roger C. Prince, Exxon Research and Engineering, Annandale, New Jersey 08801, U.S.A.

Harold Raveche, Dean, School of Sciences, Rensselaer Polytechnic Institute, Troy, New York 12180-3590, U.S.A.

John C. Salerno, Departrnent of Biology, Rensselaer Polytechnic Institute, Troy, New York 12180-3590, U.S.A.

D. Rao Sanadi, Departrnent of Cell Physiology, Boston Biornedical Research Institute, Boston, Massachusetts 02114, U.S.A.

Charles P. Scholes, Departrnent of Physics, State University of New York at Albany, Albany, New York 12222, U.S.A.

Michael Searnan, Laboratory of Bioenergetics and Departrnent of Chernistry, State University of New York at Albany, Alabany, New York 12222, U.S.A.

Jarnes Siedow, Departrnent of Botany, Duke University, Durharn, North Carolina 27706, U.S.A.

Ernest L. Siew, Departrnent of Chernistry, State University of New York at Albany, Albany, New York 12222, U.S.A.

Symposium Participants 595

E. C. Slater, Department of Biochemistry, The University of Southampton, Southhampton S09 3TU, England

Charles J. Stewart, Department of Chemistry, San Diego State College, San Diego, California 92115, U.S.A.

Hiroshi Suzuki, Department of Biomedical Chemistry, Faculty of Medicine, Nagoya University, Nagoya 466, Japan

Shigeki Takemori, Faculty of Integrated Art and Sciences, Hiroshima University, Hiroshima 730, Japan

Henry Tedeschi, Department of Biological Sciences, State University of New York at Albany, Albany, New York 12222, U.S.A.

Bernard Trumpower, Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, U.S.A.

C. L. Tsou, Institute of Biophysics, Academia Sinica, Beijing, People's Republic of China

Gebhard von Jagow, Department of Physical Biochemistry, University of Munich, Munchen 2, Federal Republic of German

Bonnie Ann Wallace, Department of Chemistry, Rensselaer Polytechnic Institute, Troy, New York 12180-3590, U.S.A.

Joseph Warden, Department of Chemistry, Renssealer POlytechnic Institute, Troy," New York 12180-3590, U.S.A.

Yau-Huei Wei, Department of Biochemistry, National Yang-Ming Medical College, Taipei, Taiwan, ROC

John Whitmarsh, Department of Plant Biology, University of Illinois, Urbana, Illinois 61801, U.S.A.

David F. Wilson, Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, U.S.A.

Daniel Wulff, Dean, College of Science and Mathematics, State University of New York at Albany, Albany, New York 12222, U.S.A.

Kunio Yagi, Institute of Applied Biochemistry, Yagi Memorial Park, Gifu 505-01, Japan

Yan Xu, Laboratory of Bioenergetics and Department of Chemistry, State University of New York at Albany, Albany, New York 12222

Aixia Zhang, Laboratory of Bioenergetics and Department of Chemistry, State University of New York at Albany, Albany, New York 12222, U.S.A.

INDEX

2, 3, 5-triphenyltetrazolium chloride, 259, 260

5,10-methenyl­tetrahydromethanopterin, 366

7-mercaptoheptanoylthreonine phosphate, 365

7-thioguanine, 195 8-chloroflavin, 58

a-tocopherol, 289 a-tocopheroquinone, 289

A23187, 402 Acamelin, 474 Acetonyldethio-CoA (Acetono-CoA),

481 citrate synthase inhibition

483, 484 synthesis, enzymatic, 482 structure, 482

Acyl CoA esters inhibition of ADP/ATP

translocase, 107 ADP/ATP exchange carrier

or ADP/ATP translocase or ADP/ATP carrier, 389, 391,

393, 394, 395, 396, 397 Adrenal cortex, as steroidogenic

ce1l type, 517, 520 Adriamycin, 409 Alkyl guanidines, 409 Alkyl-substituted benzoquinone,

287, 288 Antimycin, 147, 276

effect on cytochrome b spectra in Q-deficient yeast mutants, 147, 148, 151

Aortic endothelium as injured by lipid peroxides,

553-557 Archaebacteria, 363 Arginase, Tench liver, 487-496

kinetic properties, 494-495 physicochemical properties,

494-495

597

purification of isozymes, 488 Ariboflavinosis

role of lipid peroxides in the pathogenesis of, 560

Arum maculatum, 228, 232 mutant TZN-200 of, 259-265

Avidin-biotin-agarose, 186 Azolectin, 312 Azotobater vinelandii, 259

b-cycle, 34-39, 333 Bovine serum albumin

reversal of inhibition by long alkyl chain compounds on activities of submitochondrial particles, 109

Brown adipose tissue mitochondria, 389

Butyl gallate Candida utilis, 115 inhibitor of cyanide-resistant

pathway in plant mitochondria, 227, 228

Calcium effect on the activity of

cytochrome oxidase, 203-214

sperm motility is regulated by Ca2+, 212

Carrier proteins energy relationships, 389-399

CARS (coherent anti-Stokes Raman spectroscopy) of flavodoxin, 261, 263

CCCP, 409 Chemiosmotic mechanisms, 31-39 Cho1esterol 7 a- hydroxylase, rat

liver microsomal, 535 reconstituted activity of 535,

537 regulation of, 535-539

Claussequinone, 474 Coenzyme M, 364 Coenzyme Q (see also ubiquinones)

598

biochemistry of, 544-545 in relation to cardiomyopathy,

545-547 in relation to muscle

dystrophy, 547-551 mobile carrier concept, 142,

151 overview of research on, 543-

551 Coenzyme Q analogues

effect on succinate cytochrome c reductase

activity of wild type and Q­deficient mutants, 144

reconstitution of proton pumping in Q-deficient yeast mutants, 145-147

Coenzymes, as found in methanogens, 364

Conformational drift in methanogens, 380

Core proteins, 155, 157 Corpus luteum, as steroidogenic

cell type, 517, 520 Cyanide-resistant electron

transfer pathway, 227-233

maternal pattern of inheritance, 227, 231

radiation inactivation analysis of, 227, 231-232

Cyclic photophosphorylation, 291, 295

Cytochrome a, as site of proton translocation, 226

Cytochrome b, 141, 155, 157, 158, 162-164

electron transfer and spectral changes in, from Q­deficient mutants of yeast, 141-143

reduction by succinate in Q­deficient yeast mutants inhibition by myxothiazol, 148, 149

in deletion mutants of yeast mitochondria, 129-140

purification of, from Paracoccus denitrifi­cans, 277-278

Cytochrome b-c, complex, (see also ubiquinol cytochrome c reductase)

from Paracoccus denitrificans, 274-284

H+/e- stoichiometry of, 335 Cytochrome b.f complex

Index

electron transfer in, 291-297 Cytochrome c

carboxydinitrophenyl derivatives of, 185

nitrophenylazido derivatives of, 220

of Saccharomyces cerevisiae, 219

thiopropionyl derivatives of, 185-192

Cytochrome c" 155, 157, 158, 167, 179

autooxidation of, 180, 182 cytochrome c,-c (or C,-Hp-c)

complex, 173, 176, 179 cytochrome (c,·c) or (C,-Hp·c)

comp1ex, 176 ·one band", 167, 168 pCMB-treated, 180, 181 photoreduction, 174 subcomplex, 179 ·two band", 167, 168

Cytochrome cCm' 249, 252, 253 Cytochrome oxidase

activity of monomeric oxidase, 221

affinity chromatography procedure for isolation of, 219

cytochrome c binding site, 220 cytochrome c peroxidase

activity of, 323-332 carbon monoxide inhibition,

329-330 coupled to proton enjection

and membrane potential formation; 326-327

cyanide inhibition, 325, 328 possible coupling to proton

pumping, 323-332 effect of free fatty acids on

conformation of, 223 effect of Mg2+ and Ca2+ on, 340,

341, 332 effect of pH on activity of,

301-309, 342, 343 from boar sperm, 203-217 from Paracoccus denitrificans,

219, 221 interaction with cytochrome c

possible effect on Mg2+ and Ca2+ on, 340

matrix as source of protons in 02 reduction, 355

of subunit III deficient preparations, 301, 304

proteoliposomal, 311-321 control by ~pH, 311-321

effect of valinomycin on, 316 proton tranlocation, 31-34,

222, 301, 304, 336-343 inhibition by DCCD, 222, 305 "0 loop" mechanism, 31-34 "0 cycle" mechanism, 31-34 site of proton translocation,

223 protonmotive activity of 335-

339 H+/e- stoichiometry for, 335-

338 pulse field-sweep EPR spectrum

of, 441, 442 pulsed cytochrome oxidase, 33 subunits

monoclonal antibodies of, 193-202

three-dimensional­reconstruction of cytochrome oxidase vesicle, 461

Cytochrome oxidase, oriented membranous

EXAFS spectra of, 432-434 XANES spectra of, 431, 432 x-ray absorption studies, 429-

438 Cytochrome P-450

interaction of steroid substrates with liposomal P-450, 527-533

Daunomycine, 409 DCCD, see dicyclohexyl-

carbodiimide DCCD binding protein, 161 Deazaflavin, 260 Deoxycholate (DOC)

effect on submitochondrial respiration, 109, 110

Desulforvibrio, see sulfate reducing bateria

Desulforvibrio desulfurieans, 249 Desulfoviridin, 252 Dicyelohexylearbodiimide (DCCD),

222, 302, 335, 401-407 Diethylstilbestrol (DES)

an inhibitor of some ion­translocating ATPases, 373

Diffusion coefficients of ubiquinones, 92 measurements, 91

Diffusion constants (0) of mitochondrial redox

components, 63, 79 three dimensional D for

Index

cytochrome e, 64 Diffusion-limited reaction

of ubiquinol cytochrome e reductase, 95-97

Digitonin, 409, 411 Dimethylformamide, 186 Dodecyl maltoside, 274

599

Electrochemical studies of ubiquinone, 469-478

Electron microscopy; new methods of data analysis, 4-7

correlation averaging, 462-464 single particle averaging,

464-465 Electron microscopy, of membrane

transport and respiratory chain complexes, 459-467

bacteriorhodopsin, 459 cytochrome oxidase

three dimensional reconstruction of cytochrome oxidase vesicle, 461

ice-embedding technique, 461 nuclear pore complex, 459,

464, 465 purple membrane protein, 460

Electron spin echo envelope modulation (ESEEM) sprectroscopy, of Iron­sulfur clusters, 449-458

ELISA, 194 Endothelial cells

damage to membrane caused by lipid peroxides, 557-559

Energy coupling chemiosmotic model of, 112,

113 Escheriehia eoli, 260, 369, 371 EXAFS, see x-ray absorption

spectrocopy

F,-ATPase, 357-362 Factor Fuo ' 364 Factor F430 , 364 Factor B (F B)

inhibition by 4-vinylpyridine, 360

N-terminal amino acid sequence of, 361

role in Fa proton channel, 357-362

Fatty acids, free as preventing conformational

transition of induced by membrane potential in

600

cytochrome oxidase, 223 effect on cytochromes, 110 effect on mitochondria1

ATPase, 110 effects on NADH and succinate

oxidase, 107-114 inhibitory effect on

mitochondria, 107 uncoup1ing effect on

mitochondria, 107 Fatty acids, unsaturated

inactivate photosystem 2 by, 285-290

e1aidic acid, 286, 287 1inolenic acid, 285 linoleic acid, 286 oleic acid, 286, 287 petroselenic acid, 286, 287

Ferredoxin, 291, 292 Flavodoxin, 259-265 Flavone, 118 Flavoproteins

correlation of enzyme structure with function, 57-60

Fluorescein-phosphatidyl ethanolamine as an indicator of tntravesicular pH, 222

Glucose-6-phosphate dehydrogenase radiation inactivation

analysis of, 231, 232 Glutathione reductase, 58 Glyceralehyde-3-phosphate

dehydrogenase conformational changes of

active site in guanidine sOlution, 507-515

Gycoprotein of Influenza virus, 569

Glycoprotein of Sendai virus, 569

Hammett equation, 470 Hardt equation, 67, 78 Harmaline, 373 HHNQ, 292, 293, 295

as an analog of antimycin, 296 Hinge protein, the, 161, 167, 179

absorption and circular dichroic spectra at uv, 171

methods of preparation, 168 role of (or function of), 173-

177, 179-184 sequence, 169

HOQNO, as an inhibitor to NADH

Index

oxidation in T.

thermophilus, 246 Hydrogenase, 252, 253 Hydrogenase A, 364, 365 Hydropathy plot, 160

of six small subunits in cytochrome c reductase, 161

Intramitochondrial pH, 347-356 dependence of 3-0H-butyrate

dehydrogenase on, 347-351

effect on energetics of oxidative phosphoryation, 353-355

Iodoacetamide, 58 Iron-sulfur clusters

in bovine heart succinate dehydrogenase, 449-458

in Spirulina platensis, 449-458

Karl Popper, 13 Klebsiella pneumoniae, 259

Lateral diffusion role in mitochondrial electron

transport, 75-85, 87-105 Lauryl amine, 108 Lauryl maltoside, 302 Leydig cell as steroidogenic cell

type, 517, 520 Ligand binding reaction, rapid,

419 cryospectrophotometry in, 420 ligand "docking" site in the

reaction of Mb and CO, 419, 427

time-shared x-ray and optical measurements in, 424

x-ray absorption spectroscopy in, 419, 424

Lipase, of Candida cyclindrocea purification, 499-500

activity, stability in miscible organic solvents and at oil­water interfaces 497, 500, 501

Lipid peroxides as causing membrane damage

553-560 Liposome (see also vesicle)

as a molecule delivery vehicles to animal cell, 569-586

preparation of liposome, 569-

586 preparation of proteoliposome,

572 reconstitution of membrane

proteins into large liposome, 569-586

Sendai virus glycoprotein, 569-575

Loop gap resonated technology, 444

Manganese oxidizing bacteria, 267-272

Arthrobacter siderapsu1atus, 267

electron transfer components of, 267-272

Leptothrix pseudoochracea, 267 Meta11ogenium, 267

Membrane potential (äv) coupled to NADH oxidase in T.

thermophi1us, 246 in cytochrome peroxidase, 326

Menaquinone, 252 Mercaptoflavins, 58, 59 Metaphysical research program, 13 Methanobacterium

thermoautotrophicum, 364, 372, 373, 375, 376, 378

Methanobacterium formicicum, 364 Methanococcus vo1tae, 363, 373,

376, 377, 378 Methanosarcina barkeri, 368, 377,

378 Methanofuran, 364 Methanogenic bacteria

energy transduction in, 363-387

Methyl reductase B, 365-368,376 Methylmethanethiosulfonate, 58 Methylviologen, 250 MGBG, 409 Mitochondria, plant

cyanide-resistant oxidase of, 227-233

Mitochondria, yeast coenzyme Q deficient mutants

of, 141-153 deletion mutants of yeast bCl

complex, 129-140 purification of NADH:Q6

oxidoreductase, 115-119 Western blot analysis of, 136 Saccharomyces cerevisiae, 115-

119, 141-153 Mitochondrial electron transport

random collision model, 61-74

Index

lateral diffusion in, 75-85 effect of sucrose on, 82, 83

Mitochondrial K+ transport, 401-408

601

activation by sulfhydryl reagents, mersalyl, Cd2+, phenylarsineoxide, 401

competitive inhibition by Mg2+, Tl+ and Ba2+, 401

inhibition by quinine, 401 in relation to chemiosmotic

theory, 402 role of 82 kDa protein in, 401-

407 Mitochondrial outer membrane,

409-416 voltage dependent channels, 415

Mitochondrial respiration . inhibition by anticancer drugs,

409 role of outer membrane lysis,

409-416 Monoclonal antibodies

of cytochrome oxidase purification, 199

Myristic acid effect on submitochondrial

particles, 107, 108 Myxothiazol, 150, 276

induction of a "red shift" in cytochrome b spectrum in Q-deficient yeast mutants, 150-151

NADH cytochrome c reductase activity at different levels of

phospholipids and ubiquinones, 102

NADH:Q oxidoreductase of Paracoccus denitrificans,

237-242, 247 of Thermus thermophi1us, 237,

243-247 potentiometric titration, 240,

241, 244, 245 studies on site I from selected

bacteria, 237-248 NADH:Q6 oxidoreductase

from Saccharomyces cerevisiae, 115-119

Near equilibrium model of the first two phosphorylation sites, 348-350

Neurospora crassa, 231 Neurospora crassa, electron

micrograph of ice-embedded mitochondrial outer membrane, 463

602

NHS-SS-biotin reaction with cytochrome e,

186 Nickel

as a component of a variety of redox enzymes, 364

Nitrogen dioxide (N02 )

lung damage caused by inhalation of, 564-565

redox p~oducts and toxicity of, 561-567

Nitrogen pentoxide (N20s)' 562-563

Nitrogenase, 259 Nitroxide~spin probes

EPR relaxation times sensitive to disso1ved oxygen, 443

Nitrogert trioxide (N03), 563

Oxidative phosphorylation ro1e of intramitochondria1 pH

in the energetics of, 353-355

stoichiometry for the first two sites, 355

Ozone (03) synergistic toxicity with N02 ,

561, 564-566

Paraeoeeus denitrificans energy transduction in, 273-

284 Palmitoyl-carnitine, 109 Palmitoyl-CoA, 109 Phenylmethylsulfonylfluoride

(PMSF), 269 Pheny1-Sepharose CL-4B

QP-N purification by, 122, 123, 124

Photochemieal smog, 562-565 Photosystem 2

inhibition by unsaturated fatty acids, 285-290

quinone displacement hypothesis, 285, 287

Photosystem I, 291, 295 Pi-ATP exchange activity

Cd2+ effect on, 357, 358 inhibition by 4-vinylpyridine,

360 Piericidin, 115

• inhibition of NADH oxidase in T. thermophilus, 242

Pisum sativum inheritance of cyanide-resis­

tant respiration, 231 Primin, 474 Protonmotive aetivity, 335-339

Index

Protonmotive stoichiometry, 29-31 Proton-pumping mechanism

and eytochrome e peroxidase activity, 323-332

b eyele, b pump, 34-39, 333 inhibition of proton pump by

DCCD, 222 of eytoehrome b, 34-39 of cytoehrome oxidase, 31-34 protonmotive Q cycle, 36-48,

278-281, 333 Q-gated proton pump model, 334

Pulse field-sweep EPR, 439-443 Pyranine, 311

Q cycle, protonmotive, 36-48, 278-281, 333

QH2 : cytochrome e oxidoreductase (see also ubiquinol cytochrome c reductase)

study of recombinant yeast mutants showing which subunits are required for activity, 129-140

Q gated proton pump, 333, 334 "Q pool" hypothesis, 228 (see

also ubiquinone) QP-C, 121, 123, 126, 160, 161 QP-N, 121-127 QP-S, 121 Quinine, 401, 402, 403, 407

Random co1lision model (see also mitoehondrial e1ectron transport), 61, 75

Reconstituted glycoproteins vesicle, 580-583

binding and agglutination, 580 fusion and de1ivery of vesicle

contents, 581-582 hemo1ysing activity, 580-581

Reconstitution evolution of, as a research

program, 13-20 Respiratory chain concept

evolution of, 26-31 Rieske iron-sulphur protein, 157,

158, 159 in Paracoccus be, complex, 276 sequence comparison of, from

Nerospora crassa, Saceharomyces carlsbergenesis, Rhodobaeter capsulatus and bovine heart, 158, 159

Rotary dia lysis as a technique of vesicle

preparation, 570

Salicylhydroxamic acid (SHAM) as inhibitor of cyanide­resistant oxidase of plant mitochondria, 227

Semiquinone cycle, 333 Singer-Nicholson membrane model,

17 Single site gated pore mechanism,

391, 394 Site directed mutations, 281 Site modeling analysis in XAS

data, 435-436 XANES specta of, 431, 432

Sodium cycles role in methanogens, 369-374

Sodium dodecyl sulfate effect on submitochondrial

respiration, 109, 110 Sodium propionate

manipulation of intramitochondrial pH by, 350

Southern blot analysis, 134, 281 of yeast mutants b-c, complex,

134 Sperimidine, 409, 411 Spine probe oximetry, 443-447 Stellacyanin, 435, 436, 442 Steroid hormone synthesis,

mitochondrial, 517-525 role of certain phosphorylated

and dephosphorylated proteins in, 517-525

Stigmatelin, 245, 276 effect on NADH oxidase

activity in T.

thermophilus, 245-246 Streptococcus faecalis, 369, 371,

374 Submitochondrial particle, 107

effects of long chain alkyl compounds on, 107-114

Succinate dehydrogenase EPR spectra of, 450, 451

Sulfate reducing bacteria electron transfer in, 249-258

Sulfhydryl modified cytochrome c, 185-192

TEMPONE 439, 443, 444, 445 TMPD, 311, 312

Ubiquinol:cytochrome c reductase constituent proteins, 155-165 kinetic constants in bovine

heart mitochondria, 97

Index

structural predictions for cytochrome b, 162-164

structural predictions for non-catalytic subunits, 160-162

Ubiquinone (see also coenzyme Q) pool function of, 87-105 diffusion coefficients, 92 collisional quenching

constants, 92

603

proposed location in lipid bilayer, 94

Ubiquinone-binding protein (QP) in cytochrome bc, complex, 121,

123, 126 in NADH-ubiquinone reductase,

121-127 Q-binding protein(s) at center

i, 151 Ubiquinone-binding proteins,

effect of, on the redox properties of ubiquinone, 469-478

UHDBT, 276, 292, 293, 295, 296 Uncoupling protein, 389, 393,

395, 396, 399 Urkingdom, 363

Vectorial group translocation improbability of, 394, 398

Vectorial proton pump mechanisms of cytochrome b, 34-39

Vesicles (see also liposome) calcium-phospholipid-protein,

572 density gradient fractionation

of, 574-575 encapsulation efficiency of,

578-580 morphology of, 578 of phosphatidylserine­

cholestrol, 578 of viral glycoprotein, 569-586 sonication of, 575

Western blot analysis, 133, 193 of clones and subclones of

antibodies to cytochrome oxidase subunits, 195, 196, 197

of yeast mutants b-c, complex and mitochondria, 133, 135, 136

X-ray absorption spectrocopy, 419-428, 429-438