UltraSafe Class II BSC

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    Our experience of 40 Years brings innovation to you

    UltraSafeClass II biological safety cabinets deliver maximum safety, comfort

    and convenience. Featuring an advanced design for easy operation and maximum safety,our UltraSafe cabinets may be used wherever protection is of paramount importance insafety levels 1 to 3, including research, pharmaceutical quality control labs, pharmacies,and clinics/hospitals.

    Superior Personnel and Product Protectionwith best-in-class air flow and filter technology.

    Exceptionally Comfortableergonomic design prevents user fatigue and promotes safe

    working habits.

    Maximized Energy Efficiency for environmental protection and significant cost savings.

    Features.

    1. A clever interlocking switch design prevents accidental exposure to UV light. Should the front

    window be opened whilst the cabinet is in use an override program ensures your safety by

    automatically adopting a class 1 bio-safety mode. This feature will auto-cancel when the front

    window is closed again.

    2. The modern, proven control panel provides one touch access to all functions including servicing.

    A fully integrated self-diagnostic processor with digital status display backed up by an audible and

    visual alarm guarantees your safety.

    3. An internally mounted, exhaust HEPA filter adds to the stylish features and modern design of the

    cabinet by freeing the outer shell from protrusions.

    The supply filter provides ISO Class 3 (per ISO14644.1) and US Class 100clean air to the work surface in a

    gentle vertical laminar flow for product protection.

    4. The work area is large, airy and bright. Light pours in through large toughened glass sides and a wide

    pneumatically assisted front window.

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    UltraSafe.5. An ergonomically designed sloping front

    and new flat front intake grille allows for optimum

    operator comfort and prolonged use with ease.

    With the BSC work surface height at 900mm above

    the floor, and the seat heights set as above, the reach

    onto and over the work surface can be estimated

    when reaching into the cabinet at the arm extension

    of 120. Generally speaking, the farther a person can

    reach into the BSC without awkward positioning, the greater flexibility and access that person has to the interior

    workspace.

    These guidelines on proper seat adjustment, while relating to only one aspect of BSC ergonomics, will allow the

    user to optimize the work height of the cabinet in order to maximize reach and usability of the BSC. The

    UltraSafeBSC, when optimized as a complete workstation, will accommodate one of the farthest reaches into

    the working chamber of any BSC offered today.

    6. A large front opening (210mm) provide a comfortable and easy working access for the operator whilst

    still ensuring sterility and containment.

    7. Heavy gauge stainless steel and superior materials are used throughout. Direct drive fans (2 or more),

    dynamically balanced for vibrations free operation, ensure large flow reserves for years of trouble-free

    operation.

    8. All electrical components installed during manufacturing are CE Compliant in accordance to current

    EN Standards.

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    UltraSafe.Performance and reliability are guaranteed by full compliance with AS 2252 Part 2. 2009

    All components meet or exceed applicable safety requirements.

    Each cabinet is individually factory tested for operational performance and electrical safety.

    Documentation specific to each cabinet serial number is maintained on file

    The newUltraSafe cabinets also meet and exceed the performance requirements of EN12469

    & NSF 49-Annex F. safety and performance requirements.

    Inflow of room air enters the front air grille to establish operator protection;

    room air does not enter the work zone, preventing product contamination.

    The inflow velocity, down flow velocity,

    airflow path and intake geometry are

    precision tuned and tested to create an optimum

    air curtain on the front aperture, this curtain

    maintains personal and product protection

    even in the unlikely event of a severe inflow or

    down flow imbalance that, would compromise

    protection in a conventional cabinet.

    The combination inflow and down flow air streams

    forms an air barrier that prevents work surface emissions from

    escaping the work zone.

    Contaminated Air In

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    10

    11. HEPA FILTER

    HEPA FILTER

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    Air Barrier Containment

    Maged Shenouda,2Sherry Randhawa

    Institute of Medical and Veterinary Science, Adelaide, South Australia.Flinders University of South Australia

    Faculty of Science and Engineering, ENGR4508 Engineering Honours Project

    AbstractThe aim of this project is to investigate the various methods

    sed indetermination air barrier containment of BiologicalSafety cabinets and validate their effectiveness. The Projects

    esearch showed that the aerosol test used in determination

    f the air barrier containment should be endorsed in the

    Australian standard as a valid method of testing.

    IntroductionBiological safety cabinets have proven to be an essential

    em of equipments inside any research and or diagnosticsaboratories. Our dependency on them has improved

    rastically. Cabinets are essential component in handling

    iological hazardous materials. It is the only mean of safety

    rom getting infected with dangerous substance that

    ontribute to a high death rate that can be prevented simply

    y just using proper biological safety cabinets. Thisesearch focused on the air flow inside the cabinet and how

    could affect the air barrier at the sash of the cabinet. In

    ddition to establishing a methodology of validating theAerosol test.

    MethodsTest 1

    Setting up the cabinet according to the Britishstandard.

    Test the cabinet using potassium Iodide Discusmethod. Examine the environmental factors thatmay affect the cabinet.

    Examine and observe the droplets affect of the KI.Examineand observe the droplets affect of the KI. scrutinize the effectsof varying the velocity of the main fan motor and the exhaustfan motor on the performance of the air barrier and on theperformance of the potassium iodide discus results.Investigate the operator protection factor.

    On the original setting of the cabinet in accordance to theBritish standard test the cabinet using the smoke generatortest (the aerosol test). Examine the outcomes and correlatethe results.

    Determine the differences and the similarities and determineif the test pass or fail. Enhance the photometer with the useof particle counter in order to achieve a quantitative results.

    Repeat the test with setting up a cabinet in accordance to theAustralian standard then start to test it using the potassiumiodide discus. Produce a graph of comparison of the suitable

    air velocity that could be used where both tests fails/ passes.

    Outline the findings in terms of performance and airvelocities.

    Aero so l liq uid test

    Cheaper equipmentmulti-purpose

    Short down time to thecabinet

    Using adequate airvelocity 0.4m/sec to0.45m/sec

    Simulating operatorsmovementsTesting along the wholesashPenetrations of smokeparticles

    Particle size consistentless than 0.3 m

    Real time scanning

    KI discus test

    The cost of the equipment

    The time taken to set up, andthe general down time to the cabinet

    The use of low air velocity 0.3 m/sec

    The metal cylinder is fixedwhich doesnt represent the actual action of the operator

    The localised position of the samplers at the centre.

    The droplet effects of un- evaporated KI

    Particle size range from 4 to 10 m

    No substance reference to OPF of 10^5

    Test 2:

    Scattering effect around the cabinet.

    Use a die to observe the scattered potassium iodidearound the cabinet.

    Is it sufficient to test the centre only?

    Move the sampler of the KI discus towards the side ofthe cabinet and carry out a test. Work out the operatorprotection factor at the new positions of the samplers.

    Correlate the results obtained versus the resultsobtained from testing at the centre only. Investigate theareas where the barrier is vulnerable.

    Pump

    Op. virt . arm

    KI nozzle.

    s

    N

    Mn

    Disc

    Air s amplers

    Test 3:

    Work out the centripetal force that the particles hittingthe barrier with for both the KI discus method and theaerosol liquid method.

    For the KI discus method

    38mm spinning disc (28,000 r/min) + nozzle deliveringM=20ml of 15g/l solution of KI, generating N particles

    Air samplers s=100dm3/min with 25mm filter

    membranes Count the spots on developed membranes: n.

    Calculate Apf:

    with N = 3.1 x 107x M

    Apf = = Ns/104n

    If n = 62, Apf= 1 x 105

    If n = 1, Apf= 6.2 x 106

    Correlate with the force of generating aerosol.

    Results

    By lowering the speed of the

    main fan

    Average air velocity of 1.12 m/s

    1.6

    1.4

    1.2

    1

    0.8

    0.6

    0.4

    0.2

    0

    Inflowairvelocityofa BSC

    Inflow air at the

    opening of the

    cabinet

    1 2 3 4 5 6 7 8 9 10

    positionsof the anemometer

    Validation and Comparison Results

    ConclusionThe Key factor or controller of the air barrier is the exhaust

    fan motor. The barrier is maintained proportionally to theadjustment of the exhaust fan as shown from the results.

    lowering the air velocity of the main fan interrupt the barrier,

    and raise the main issue of cross contamination inside the

    cabinet. From the comparison performed, Aerosol liquid test

    provide better qualitative method of testing the air barrier

    since it cover lots of aspects of practical informative method

    of test. KI discus test can not be carried out at any laboratory

    since it could contaminate the work in the actual laboratory.

    In contrast to aerosol test which doesnt interfere with the

    nature of work carried out in the laboratory.

    Acknowledgement Miss Sherry Randhawa academic supervisor of the

    research

    I.M.V.S Engineering services department.

    Clyde-Apac for their contribution and providing us with the

    KI discus equipment as well as referred materials.

    References Australian standard 2252.2.

    Australian standard 1807.26-2004

    Australian standard 1807.22

    IMVS, CTL , procedure manuals for mechanical testing. ESCO Global Co-operation, Singapore.

    Published article reviews on KI discus

    [email protected]

    Now at these optimum

    conditionsTesting the inflow of the airacross the opening of thecabinet using anemometerat 10 labelled spots, hence50 mm from each sides ofthe cabinets then with in aspacing of 100 mm afterthe sides.

    Average air velocity of1.7m/s

    By lowering the speed of

    the exhaust fan

    Average air velocity of

    0.21 m/s

    Inflowair velocityof aBSC

    2

    1.5

    Inflow air at the

    1 opening of the

    cabinet

    0.5

    0

    1 2 3 4 5 6 7 8 9 10

    positions of the anemometer

    Inflow air velocity of a BSC

    0.5

    0.4

    0.3 Inflow airat the

    opening of the

    0.2 cabinet

    0.1

    0

    1 2 3 4 5 6 7 8 9 10

    positionsof theanemometer

    1 2,

    1

    2

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    UltraSafe.

    Providing a safe barrier for you to work in.

    Pressuresensormonitoringlocatedinsidethe

    airchamberensuressafeairflowsacrossthe

    entireworksurfaceoftheUltraSafe

    cabinets.

    UltraSafeusesdigitaltechnologyto

    maintainconstantairflowduring

    normalfilterloadingortemporary

    airflowobstructionfromforeign

    objects

    Silencewithoutcompromisingcomfortand

    safety.

    Usersafetyandcomfortisparamountwithall

    AESEnvironmentalmanufacturedbiological

    safetycabinets.Thecomfortable,200mmfront

    workingapertureontheUltraSafedoesnot

    compromisesafetyorsamplecontainmentwhile

    significantlyreducingnoiselevel.

    Allofourcabinetshavebeentestedatnominal

    airflowvelocitiesforaddedsecurity.

    Customizedworksurfaces

    Scratchfree,highquality

    stainlesssteelworksurfacesare

    availableassingleor

    segmentedmodulesandare

    easytocleanorautoclave.The

    indentedworkmoduleseasily

    trapspilledliquids.

    ThetransparentsideUV

    resistantsafetyglass

    maximizelightandvisibility

    insidethecabinet,providing

    abrightandopenworking

    environment.

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    UltraSafe.

    PowerfulUVirradiation

    illuminatestheentire

    workingareawithout

    causingshadows.

    Thisensuresthorough

    disinfectionofthe

    completeworkchamber

    formaximumdisinfection

    Thehingedfrontwindowcanbe

    fullyopenedforeasyandthorough

    cleaningof

    the

    unobstructed

    rear

    wall.

    Thefullyopenedfront

    totheworkchamberfor

    largeitemsandaccessories.

    Optionalvibrationdampedwork

    surfacesectionwithgranite

    plateformaximizedstabilityfor

    sensitiveweighingapplications.

    loading andunloadingof

    window allowseasyaccess

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    UltraSafe.

    Options and AccessoriesTheheightofthefloorstandcanberaisedorloweredatthetouchofabutton

    to

    accommodate

    any

    working

    height

    between640mm1000mm.

    The low voltage keypad control panel is simple in design and easy

    to use. The control panels memory can be programmed to accept 4

    pre-set heights from 600 900mm

    ColourCodedLaboratoryTaps

    Modifications toacabinetsworktrayandviewing

    windowtoaccommodationthefittingofamicroscope

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    UltraSafe

    .

    Options and Accessories Part Numbers

    2060111 Floorstand UltraSafe90

    2060121 Floorstand UltraSafe120

    2060131 Floorstand UltraSafe180

    16875512 AutoAdjustableElectricFloorStand UltraSafe90&120

    16875518

    Auto

    Adjustable

    Electric

    Floor

    Stand

    UltraSafe

    180

    2080710 AdditionalPoweroutlet,singlesocket(GPO)

    16870100/15 GasTap&Solenoid

    16870100/45 VacuumTapwithMilliporeFilter

    16870100/25 Air&orVacuum Tap

    16870100/5

    6

    20mmGranite

    vibration

    damped

    work

    surface

    16870100/690 ModifiedFrontSafetyWindowforMicroscopeApplicationUltraSafe90

    16870100/6120 ModifiedFrontSafetyWindowforMicroscopeApplicationUltraSafe120

    16870100/6180 ModifiedFrontSafetyWindowforMicroscopeApplicationUltraSafe180

    16870100/790 2Piece LevelWorkTray UltraSafe90

    16870100/7120 3PieceLevelWorkTray UltraSafe120

    16870100/7180 3PieceLevel WorkTrayUltraSafe180

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    UltraSafe.GeneralDesignSpecificationULTRASAFEClassIIBiologicalSafetyCabinet.

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    1. UltraSafeSeries2000microprocessorcontrolsystem 7. ExhaustHEPA

    2. Externalfluorescentlighthousing 8. ExhaustFan

    3. Hingedwindow (Aerosoltight 9. LaminarFlowFan

    4. Removablesidepanelsforelectricalaccess 10. LaminarFlowHEPA

    5. Internalstainlesssteelbackandsidepanels 11. UVTube

    6. Combinedairgrilleandworktray 12. CabinetSumpCompartment

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    KDD 9/7T 350W 4P-1 3SA

    VOLT 220-240 Ph 1

    WATT 350 Hz 50

    RPM 1330/1150/1050 Cl. F uF/V 12.5/450 Date 11/5/01

    POLE 4 Temp. min -20oC N. Amp 2.4 Weight 12.6 kg

    IP 21 max +40oC M. Amp 3.3

    All Dimension in mm .

    * Sound Lp(A) at 1 m free field.

    * Performance shown is for installation type B - free inlet, ducted outlet

    SL. KD09003.3

    Part No. CE-AD1F723.01

    Wiring diagram

    232 85317

    152

    262

    327

    185

    39

    Low

    Med

    Hi

    Capacitor

    Green-Yellow

    Red

    Brown

    Blue

    Black

    White

    L

    N

    HIGH

    MEDIUM

    LOW

    65.858.551.4

    67.2

    68.1

    64.1

    60.7

    64.7

    64.4

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    AES EnvironmentalGENER L SPECIFIC TION ULTR S FE CL SS II BIOLOGIC L S FETY C BINET

    Model(s) UltraSafe90T UltraSafe120 UltraSafe180

    PartNumber(s) 16876000/90T 16876000/120T 16876000/180T

    NominalSize(s) 0.9meters(3) 1.2meters(4) 1.8meters(6)

    CabinetExternalDimensions(WxDxH) 1035x790x1570mm 1350x790x1570mm 1975x790x1570mm

    InternalWorkZoneDimensions(WxDxH) 870x580x620mm 1180x580x620mm 1810x580x620mm

    TestOpeningAperture 210mm

    WorkingOpeningAperture 210mm

    Fans:240VSinglePhaseDirectDrive 2 2 3

    1meterpersecondatsetpoint

    0.40.45meterspersecond

    250Litrespersecond 300Litrespersecond 490

    240Litrespersecond 290Litrespersecond 470

    250Litrespersecond 300Litrespersecond 490