UltraSafe Class II BSC
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Transcript of UltraSafe Class II BSC
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Our experience of 40 Years brings innovation to you
UltraSafeClass II biological safety cabinets deliver maximum safety, comfort
and convenience. Featuring an advanced design for easy operation and maximum safety,our UltraSafe cabinets may be used wherever protection is of paramount importance insafety levels 1 to 3, including research, pharmaceutical quality control labs, pharmacies,and clinics/hospitals.
Superior Personnel and Product Protectionwith best-in-class air flow and filter technology.
Exceptionally Comfortableergonomic design prevents user fatigue and promotes safe
working habits.
Maximized Energy Efficiency for environmental protection and significant cost savings.
Features.
1. A clever interlocking switch design prevents accidental exposure to UV light. Should the front
window be opened whilst the cabinet is in use an override program ensures your safety by
automatically adopting a class 1 bio-safety mode. This feature will auto-cancel when the front
window is closed again.
2. The modern, proven control panel provides one touch access to all functions including servicing.
A fully integrated self-diagnostic processor with digital status display backed up by an audible and
visual alarm guarantees your safety.
3. An internally mounted, exhaust HEPA filter adds to the stylish features and modern design of the
cabinet by freeing the outer shell from protrusions.
The supply filter provides ISO Class 3 (per ISO14644.1) and US Class 100clean air to the work surface in a
gentle vertical laminar flow for product protection.
4. The work area is large, airy and bright. Light pours in through large toughened glass sides and a wide
pneumatically assisted front window.
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UltraSafe.5. An ergonomically designed sloping front
and new flat front intake grille allows for optimum
operator comfort and prolonged use with ease.
With the BSC work surface height at 900mm above
the floor, and the seat heights set as above, the reach
onto and over the work surface can be estimated
when reaching into the cabinet at the arm extension
of 120. Generally speaking, the farther a person can
reach into the BSC without awkward positioning, the greater flexibility and access that person has to the interior
workspace.
These guidelines on proper seat adjustment, while relating to only one aspect of BSC ergonomics, will allow the
user to optimize the work height of the cabinet in order to maximize reach and usability of the BSC. The
UltraSafeBSC, when optimized as a complete workstation, will accommodate one of the farthest reaches into
the working chamber of any BSC offered today.
6. A large front opening (210mm) provide a comfortable and easy working access for the operator whilst
still ensuring sterility and containment.
7. Heavy gauge stainless steel and superior materials are used throughout. Direct drive fans (2 or more),
dynamically balanced for vibrations free operation, ensure large flow reserves for years of trouble-free
operation.
8. All electrical components installed during manufacturing are CE Compliant in accordance to current
EN Standards.
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UltraSafe.Performance and reliability are guaranteed by full compliance with AS 2252 Part 2. 2009
All components meet or exceed applicable safety requirements.
Each cabinet is individually factory tested for operational performance and electrical safety.
Documentation specific to each cabinet serial number is maintained on file
The newUltraSafe cabinets also meet and exceed the performance requirements of EN12469
& NSF 49-Annex F. safety and performance requirements.
Inflow of room air enters the front air grille to establish operator protection;
room air does not enter the work zone, preventing product contamination.
The inflow velocity, down flow velocity,
airflow path and intake geometry are
precision tuned and tested to create an optimum
air curtain on the front aperture, this curtain
maintains personal and product protection
even in the unlikely event of a severe inflow or
down flow imbalance that, would compromise
protection in a conventional cabinet.
The combination inflow and down flow air streams
forms an air barrier that prevents work surface emissions from
escaping the work zone.
Contaminated Air In
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11. HEPA FILTER
HEPA FILTER
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Air Barrier Containment
Maged Shenouda,2Sherry Randhawa
Institute of Medical and Veterinary Science, Adelaide, South Australia.Flinders University of South Australia
Faculty of Science and Engineering, ENGR4508 Engineering Honours Project
AbstractThe aim of this project is to investigate the various methods
sed indetermination air barrier containment of BiologicalSafety cabinets and validate their effectiveness. The Projects
esearch showed that the aerosol test used in determination
f the air barrier containment should be endorsed in the
Australian standard as a valid method of testing.
IntroductionBiological safety cabinets have proven to be an essential
em of equipments inside any research and or diagnosticsaboratories. Our dependency on them has improved
rastically. Cabinets are essential component in handling
iological hazardous materials. It is the only mean of safety
rom getting infected with dangerous substance that
ontribute to a high death rate that can be prevented simply
y just using proper biological safety cabinets. Thisesearch focused on the air flow inside the cabinet and how
could affect the air barrier at the sash of the cabinet. In
ddition to establishing a methodology of validating theAerosol test.
MethodsTest 1
Setting up the cabinet according to the Britishstandard.
Test the cabinet using potassium Iodide Discusmethod. Examine the environmental factors thatmay affect the cabinet.
Examine and observe the droplets affect of the KI.Examineand observe the droplets affect of the KI. scrutinize the effectsof varying the velocity of the main fan motor and the exhaustfan motor on the performance of the air barrier and on theperformance of the potassium iodide discus results.Investigate the operator protection factor.
On the original setting of the cabinet in accordance to theBritish standard test the cabinet using the smoke generatortest (the aerosol test). Examine the outcomes and correlatethe results.
Determine the differences and the similarities and determineif the test pass or fail. Enhance the photometer with the useof particle counter in order to achieve a quantitative results.
Repeat the test with setting up a cabinet in accordance to theAustralian standard then start to test it using the potassiumiodide discus. Produce a graph of comparison of the suitable
air velocity that could be used where both tests fails/ passes.
Outline the findings in terms of performance and airvelocities.
Aero so l liq uid test
Cheaper equipmentmulti-purpose
Short down time to thecabinet
Using adequate airvelocity 0.4m/sec to0.45m/sec
Simulating operatorsmovementsTesting along the wholesashPenetrations of smokeparticles
Particle size consistentless than 0.3 m
Real time scanning
KI discus test
The cost of the equipment
The time taken to set up, andthe general down time to the cabinet
The use of low air velocity 0.3 m/sec
The metal cylinder is fixedwhich doesnt represent the actual action of the operator
The localised position of the samplers at the centre.
The droplet effects of un- evaporated KI
Particle size range from 4 to 10 m
No substance reference to OPF of 10^5
Test 2:
Scattering effect around the cabinet.
Use a die to observe the scattered potassium iodidearound the cabinet.
Is it sufficient to test the centre only?
Move the sampler of the KI discus towards the side ofthe cabinet and carry out a test. Work out the operatorprotection factor at the new positions of the samplers.
Correlate the results obtained versus the resultsobtained from testing at the centre only. Investigate theareas where the barrier is vulnerable.
Pump
Op. virt . arm
KI nozzle.
s
N
Mn
Disc
Air s amplers
Test 3:
Work out the centripetal force that the particles hittingthe barrier with for both the KI discus method and theaerosol liquid method.
For the KI discus method
38mm spinning disc (28,000 r/min) + nozzle deliveringM=20ml of 15g/l solution of KI, generating N particles
Air samplers s=100dm3/min with 25mm filter
membranes Count the spots on developed membranes: n.
Calculate Apf:
with N = 3.1 x 107x M
Apf = = Ns/104n
If n = 62, Apf= 1 x 105
If n = 1, Apf= 6.2 x 106
Correlate with the force of generating aerosol.
Results
By lowering the speed of the
main fan
Average air velocity of 1.12 m/s
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
Inflowairvelocityofa BSC
Inflow air at the
opening of the
cabinet
1 2 3 4 5 6 7 8 9 10
positionsof the anemometer
Validation and Comparison Results
ConclusionThe Key factor or controller of the air barrier is the exhaust
fan motor. The barrier is maintained proportionally to theadjustment of the exhaust fan as shown from the results.
lowering the air velocity of the main fan interrupt the barrier,
and raise the main issue of cross contamination inside the
cabinet. From the comparison performed, Aerosol liquid test
provide better qualitative method of testing the air barrier
since it cover lots of aspects of practical informative method
of test. KI discus test can not be carried out at any laboratory
since it could contaminate the work in the actual laboratory.
In contrast to aerosol test which doesnt interfere with the
nature of work carried out in the laboratory.
Acknowledgement Miss Sherry Randhawa academic supervisor of the
research
I.M.V.S Engineering services department.
Clyde-Apac for their contribution and providing us with the
KI discus equipment as well as referred materials.
References Australian standard 2252.2.
Australian standard 1807.26-2004
Australian standard 1807.22
IMVS, CTL , procedure manuals for mechanical testing. ESCO Global Co-operation, Singapore.
Published article reviews on KI discus
Now at these optimum
conditionsTesting the inflow of the airacross the opening of thecabinet using anemometerat 10 labelled spots, hence50 mm from each sides ofthe cabinets then with in aspacing of 100 mm afterthe sides.
Average air velocity of1.7m/s
By lowering the speed of
the exhaust fan
Average air velocity of
0.21 m/s
Inflowair velocityof aBSC
2
1.5
Inflow air at the
1 opening of the
cabinet
0.5
0
1 2 3 4 5 6 7 8 9 10
positions of the anemometer
Inflow air velocity of a BSC
0.5
0.4
0.3 Inflow airat the
opening of the
0.2 cabinet
0.1
0
1 2 3 4 5 6 7 8 9 10
positionsof theanemometer
1 2,
1
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UltraSafe.
Providing a safe barrier for you to work in.
Pressuresensormonitoringlocatedinsidethe
airchamberensuressafeairflowsacrossthe
entireworksurfaceoftheUltraSafe
cabinets.
UltraSafeusesdigitaltechnologyto
maintainconstantairflowduring
normalfilterloadingortemporary
airflowobstructionfromforeign
objects
Silencewithoutcompromisingcomfortand
safety.
Usersafetyandcomfortisparamountwithall
AESEnvironmentalmanufacturedbiological
safetycabinets.Thecomfortable,200mmfront
workingapertureontheUltraSafedoesnot
compromisesafetyorsamplecontainmentwhile
significantlyreducingnoiselevel.
Allofourcabinetshavebeentestedatnominal
airflowvelocitiesforaddedsecurity.
Customizedworksurfaces
Scratchfree,highquality
stainlesssteelworksurfacesare
availableassingleor
segmentedmodulesandare
easytocleanorautoclave.The
indentedworkmoduleseasily
trapspilledliquids.
ThetransparentsideUV
resistantsafetyglass
maximizelightandvisibility
insidethecabinet,providing
abrightandopenworking
environment.
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UltraSafe.
PowerfulUVirradiation
illuminatestheentire
workingareawithout
causingshadows.
Thisensuresthorough
disinfectionofthe
completeworkchamber
formaximumdisinfection
Thehingedfrontwindowcanbe
fullyopenedforeasyandthorough
cleaningof
the
unobstructed
rear
wall.
Thefullyopenedfront
totheworkchamberfor
largeitemsandaccessories.
Optionalvibrationdampedwork
surfacesectionwithgranite
plateformaximizedstabilityfor
sensitiveweighingapplications.
loading andunloadingof
window allowseasyaccess
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UltraSafe.
Options and AccessoriesTheheightofthefloorstandcanberaisedorloweredatthetouchofabutton
to
accommodate
any
working
height
between640mm1000mm.
The low voltage keypad control panel is simple in design and easy
to use. The control panels memory can be programmed to accept 4
pre-set heights from 600 900mm
ColourCodedLaboratoryTaps
Modifications toacabinetsworktrayandviewing
windowtoaccommodationthefittingofamicroscope
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UltraSafe
.
Options and Accessories Part Numbers
2060111 Floorstand UltraSafe90
2060121 Floorstand UltraSafe120
2060131 Floorstand UltraSafe180
16875512 AutoAdjustableElectricFloorStand UltraSafe90&120
16875518
Auto
Adjustable
Electric
Floor
Stand
UltraSafe
180
2080710 AdditionalPoweroutlet,singlesocket(GPO)
16870100/15 GasTap&Solenoid
16870100/45 VacuumTapwithMilliporeFilter
16870100/25 Air&orVacuum Tap
16870100/5
6
20mmGranite
vibration
damped
work
surface
16870100/690 ModifiedFrontSafetyWindowforMicroscopeApplicationUltraSafe90
16870100/6120 ModifiedFrontSafetyWindowforMicroscopeApplicationUltraSafe120
16870100/6180 ModifiedFrontSafetyWindowforMicroscopeApplicationUltraSafe180
16870100/790 2Piece LevelWorkTray UltraSafe90
16870100/7120 3PieceLevelWorkTray UltraSafe120
16870100/7180 3PieceLevel WorkTrayUltraSafe180
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UltraSafe.GeneralDesignSpecificationULTRASAFEClassIIBiologicalSafetyCabinet.
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1. UltraSafeSeries2000microprocessorcontrolsystem 7. ExhaustHEPA
2. Externalfluorescentlighthousing 8. ExhaustFan
3. Hingedwindow (Aerosoltight 9. LaminarFlowFan
4. Removablesidepanelsforelectricalaccess 10. LaminarFlowHEPA
5. Internalstainlesssteelbackandsidepanels 11. UVTube
6. Combinedairgrilleandworktray 12. CabinetSumpCompartment
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KDD 9/7T 350W 4P-1 3SA
VOLT 220-240 Ph 1
WATT 350 Hz 50
RPM 1330/1150/1050 Cl. F uF/V 12.5/450 Date 11/5/01
POLE 4 Temp. min -20oC N. Amp 2.4 Weight 12.6 kg
IP 21 max +40oC M. Amp 3.3
All Dimension in mm .
* Sound Lp(A) at 1 m free field.
* Performance shown is for installation type B - free inlet, ducted outlet
SL. KD09003.3
Part No. CE-AD1F723.01
Wiring diagram
232 85317
152
262
327
185
39
Low
Med
Hi
Capacitor
Green-Yellow
Red
Brown
Blue
Black
White
L
N
HIGH
MEDIUM
LOW
65.858.551.4
67.2
68.1
64.1
60.7
64.7
64.4
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AES EnvironmentalGENER L SPECIFIC TION ULTR S FE CL SS II BIOLOGIC L S FETY C BINET
Model(s) UltraSafe90T UltraSafe120 UltraSafe180
PartNumber(s) 16876000/90T 16876000/120T 16876000/180T
NominalSize(s) 0.9meters(3) 1.2meters(4) 1.8meters(6)
CabinetExternalDimensions(WxDxH) 1035x790x1570mm 1350x790x1570mm 1975x790x1570mm
InternalWorkZoneDimensions(WxDxH) 870x580x620mm 1180x580x620mm 1810x580x620mm
TestOpeningAperture 210mm
WorkingOpeningAperture 210mm
Fans:240VSinglePhaseDirectDrive 2 2 3
1meterpersecondatsetpoint
0.40.45meterspersecond
250Litrespersecond 300Litrespersecond 490
240Litrespersecond 290Litrespersecond 470
250Litrespersecond 300Litrespersecond 490