U\ ~~ IU .('\J ~J ··'\ J\ .. £'\ ,•IC, I~ ..... ~H 4 ,, ,, ..J ~~ ~\ IJ .. ..,J .. ..r.~ .. .. ~ ..,y;w '-" ~ ~ .. • .. .. .. .. ....,

4;h;~\ SJJ.ll\ c) ~,Jl\ lA,JJ.lJ

Khawia lutei (Cestoda : Lytocestodae ; Caryophyllidea) •

~ [.;ill ·¥ W:!.lll c~ ~

s~lr~~

~~~~~/r~l~

~i ~ ~ ...>: ~

~L~JJI ~I r~ tJ ~~~~~~~~~~

ABSTRACT The study involved the investigation of protein and carbohydrate

substances in adult tapeworm Khawia luteiu using histochemical techniques (Ninhydrin- Sheff,Millons reagent and Toluidine-blue).

Protein and carbohydrates were found in abundance in reproductive organs ,mesenchyma tissue, vitelline glands and eggs, using Ninhydrin- Sheff and Toluidine blue stains.Also, the muscular system, mainly the longitudinal muscles, showed strong positive reaction with Ninhydrin- Sheff stain and negative reaction with Toluidine blue and Millons reagent. Positive and weakly positive reactions in different tissues and organs were noticed with Millons reagent.

The study also showed that the histochemical reactions in the worm varied in strength because the metabolic and physiological activities, such as protection, energy storing, sensitive pulses reception to activate reproductive processes and host immune response resistance.

~I

o~3.L..ll yi.J~.J:l.J.l1 3 ~3y,ll ~I_,_.JI uc l:$~1 ~l:JI ~I.J..lll WlAl :...~,

'~ _ U:u~~ Jla ~ ~~ w\Jt:ii rl~~ Khawia lutei W'-:JI ~y:;JI

.lJ:1~_,Jjlll L;.Jj\ ~3 'U.J4a ~ts

r-~C.XI. ~ 4.JJI ~ .q ~ ··'I ..ll --11 · r, ,Q ~ · ·~1 · · ~1 ~ ~ .) ~ _J;) ...)-*' .J ~ ~ .J~ .r' l)A ..r .J -» .J ~ l)A ~

~ · .l.ll.·.·. ~· 1~1 ,ljc -·- .. 11 ~I ~.li.ll _,<:\ • ... 11 _, .. :,11 ~ ~ts:i.ll ~ _ U:U ~~ ~ f' ~.J ~ .J ~~ ~-::--.J ~Y

)k.Lj:j ~ ·'-'1 ~w · ~~-: ~. 11 o..l .ill 1. ·--'1 ·t ·-'I .. 1:.1 lA5;. · ~ -' .~.11 ~ ·1 . ~ ~ . ~ .J .J ~ .)~ .A-"" rY. .Y Y' (,j.).) .J

~I,S ' ~ - I -~· 1 1 ~ 'I ~ l.JL.., )k.\j:j ~ ' .lli · ·.·. ~ . l.ll~ b .J u; .:r-' Y' (j.).) . co . .J ~ _(.);.) ~ ~ . co ~ .. _JA

~ -4 ah.:.. Jl r-L..:::u::.C.XI_, ~C.X1 ~ ~ ~.JA.J ~.JA w)k.UJ W .k_,J.J ·u.J4a

II 70 II

=====~ .... J\_,.31 <F- ".;o:.u ~ l.;lt,...;s l...\_;J lF===== 4 at.;., Wii.S o.) ..lll . _g ~I ~~I wk.\..9:JI ·1 ~I ..lll · • ·• I . · -1 · - ~\.S _9 c..r ..... • •• u ) LJA~ u~

wl,~. aa...._.,llJ•·,II J~l U\.bll · · · • 4...J~I 1•· · ~I ~'JI wl.bWI l......l..1.ul. o~ - . ....J ~_p...J .J • U.IA • . .J • • . . . .

·' "J· :-,.,JI ~\..WI ~~';II ~.JI.ia.J ~.)I.S:JI w~l ~.J ~I

~.ll.o.ll

~~ ,~.)\-·~-~"';11 ~'JI wlj ~_foJI w\JI~I 0-c ~~ ~~'JI uafi

ul....iliib wJu 1'-·' ,~1 o ···'I. _il o ... < 'L..u.;.. l......l..1.ul. ~ wl.ili9..b.ll · o.lJ.)c. tl -L • - ~ .J • .J.? c..r ~ y . . • - . LJA .. y .

~ ~411 0-c .l;!..WI ~ 0-c \....,~1 'l).l:!..lli.J JI.J';II 0-c 4-cl_il1 u~l ~ ,~~'JI

, • • ~ u"l...:,....,. _g wl.....l ..lll · 1 '·~ -' ~j N· · 1 ··- ... , ,.lwl ~l::. ... J ~ ~ .. . .:~ · r...,r .J LJA UJ r lJ-A-"'o LJA .J~ 1

•'L...i.JI ~ • . I. ''·I I. .-. II I. -.. ~\..c wl.ili9..b.ll -illJ [ ;, , ·,. a"• 1~ w.Lu..:;.l ~ .J ~ LJA .J ~ §- .J ~.) (.S", ~ .J .. .. "--"'::-.. ~ c..s--

w...JI_jj ~~. ~~ ~ J.J'JI l@ly:-·/'.J ~~ ~.J uc ~ ,~~ A.....ai...;.J\

44JW ~.J ~I l).l:!..lll -illJJ ~..lll ~_;JI 4..9y...o ~ u:o.;..{{J lSY..1 w~\.J.)

~I .J---'-'l ~l....a.c t-" 4J..;..Iij .J Wi .J ~4::.. ;; .).Jj l..F-~ '1 ('? L9 ~ ~j .).Jj .J ~~I

.(14-1) ,~4-:JI) ~)\

uL.......ui.J..lll u-c .l;!..WI uj.7.J ~, lS.# ~b.....c ~'l~.J ,pi~~\....,)

l5.l........c u,....c. ;;) ~ I.J~ w44iliJI r1=- ~ ~411 u-c jjc. ~ j.a ~~lye~

~ , ;;.l:!~ t I _il'l Li...::. .J w~l .J..lll -illJ uc ~ ~ w449bJI ' a!·-,.;, ~ JJ~ '1 I ~La) ~.J ~ lSY..i ~L....\.J.) ~1 ~ ~ ,(15) Fattohy ~ u-c ~.J'll :.i........I.J..lll L..LcJ

-16) ,4 al·-,.;.J\ ~JP.1 ~! 0~1.J ~~ c).l:!..lli.J JI.YU ~..lli.J ~l..lll ~_;JI

y.._....jl_p.JI ~ .).J'll :.i........l.)..lll u§_;b:J '~..?.1 (30,29) ~.h. ~~.).) u.J ,(28

~l..t::.. U I~ 4...J.itJ\ :.i.......,\ ..ll\ ~\ · i1 ,JSb:Jl.J 1 .··.~)k_ ~~)U ~\ .. • y...o ~ .. .) . (.);!-=>- '-:? . ~ .J ...

~ . I~ ..l..__ij LA N~ . -~ 4...J ... ~l....a.c . ~\~.=·.II '\.lJ..lll .J---".::l ~ .J ( lJ-A-"'o LJA .. ..P ~ .. ..~ u .. \..c ·;; j\_j ~\ · l.lJ..llJ.,.. ... :.1\ ~h.wS..J A.....ai...;.J\ wLu.l ..ll\ ·I L.h · .. ~b..J\ u\...., -'- -'1 Y.J ... u .. e:..._........... ....... .J u, ... lY.+l ~

~ ~ ;;~\ ~~\ ~~I w\J~I j..?..J uc ~I ~ ;;~\ ~1 ri=-.J \....,

_y..al.k.ll uc ~ , (.:.A.4:!j.i'li.J u.JA> ..lll .J w~ .Jyli.J wl .J~ y, fl~ ~~ .J ~~ ~\.SJI

.h:j 1.~ L 'I ~ L.ac.1 ~1 . j 4...J . ·- 'I .)\ . -'1 -illJ . .. ·.( - j ,q . ~\ 4...J.i.h...c..ll ~ .J . '-:? .. ~ _,..., ~.JY .J ...J-> _)A-l.J ..?. .:r .. ..

~~.J 4 ... 1... 9 ~ ;;w I.J.Jj ~~I ~';II w~l ~.J ~ 4..9y...oJ ~~ Jj';Jj

~~ ''11 WI u.lJ ~j -:..::.-., . ~ ~\.) _ .. ul a..lb il wb "~'-'1 • .. .. (.S".J . . ~.J .. ~ Y" c..r .J ~

monozoic and LH~I o.).ltia.J ~jb1 ~I ul.l;!..lll u-c lBC ~ tJ411 .J_,hjl ~ ~I

u~ ~ ~~\ .)\yJI jSy{J uc ( 31) J\...rJI w~ ~ ~' (18) polyzoic

(32) lSY..1 4.........1.Jj U.J' Echinococcus granulosus ~I .&S.ykll tj.J..WI ~I

.. \.

==========~r----~-·~-~_l _t_~ __ ._~ ___ J_~ ___ i _~_~_· _UM_~_· 1_'' __ ~]F======== <.JI.l.....;l~l wl9.>.1 ~~I (.).IIL:S\11 c)~~~ .. li_,.JI ..:l..?.J USL.ai uc u.1SJJ ~-»\

~.1:.. w~l j wll ~ > • 1 A..J .::~11 1....-Aj~\ · · . _g ~I ~I ~ . •.11 ~ .) _JJ ~ <.)A J ~~ <.)A ~ '-:? . J ~ - J - ~..r""'

w~I.J.ill l..:ll.ll.a\ .llW.I ~~.J~I o~ w~ jy, ~ 0.a 1 J~l ~c) ( 34, 33) <.SY:..i

~I ..:l ..:ll · -'1 wlili9.bll · 1 ~1 j ~~ - ... :.1\ A..Jjh..uS w\Jt:g::, · ~ ol ·- -'1 .) ..r' ~~ <..JA(.j • '-:? ,. Je:......- ~ ~ ~ L) • ~

~ 1J9bl\ ~ ~ ~y&JI ~~~ w~_?.JI j..?.J USL.ai 4.!.)'1-4 ~j U'J..:.. 0.a ~

1....-AjL.._b_,l\ u.)'l-4 4l.S...aJ ~J J...a;JJ wl.J.U3 .-r.J1 3 wi.J~y,fllS ~ ~_,ll IA.JJ..:lJ

,~,1..,. ,. ~I 4..i.l4JI w4h;y:;JI 0.a o.1:..\ 3 c) ~.h:j ~I ~I ~\.....ac~l, a\\;,J ~~~

1(19) tj~l ~~\ ~~1 <} ty.h.

~\ ~l_;bj .1\_,.J\

~~\ ~~i <} ~\ Khawia lutei w~y:;JI 0.a ~ wL;,.j~j ~

~) 1 J,..ayJI ~~ .JWI 4J.:.,..:l ~<.)a ty.1:.. o..:l~l 'Barbus luteus '-?~1

~ L.:;.__ij\ l.JI..i.J • I • ~ .1 .:_ ·.~. ~ - La J . L . _q ~ 0 · 0 ~ ,q 4...1.,~ wl.ili.J · •11 ~ . <.)A ~ ~J ~ ~ '-:? JJ ~ ../" Y. - • ..r""'

lA~ , ~WI w~~ w4h;~JI ..:..llfj . 4-:l 0JWI ' "J· ;.,"ll ~~\~ ~I ~~I

~ u.a~l ~ <.J.a3 . u3 fo.La 7 ~ )3~\ clyt.J~ ~3 ~I.J4JI ~ w~

w..:l , • \ "·'"' .... ijjl ..l.lAl ·''I 4...wJ .. 11 ..:ll . ·'I j · w..:;&ll ~I ~L..uSJI w\J\.S:j .JJ •• .J - >r~ J .. - J~ .)A-' ..?. J uc .. . .. .. ..

Ninhydrine- Schiff technique ~ - ·u.u~l+W ~ I

Millon reagent technique u.J4.o ~lS ;;,J·,;;",- 2

~~.)~-':I .fill

I..US ~~I cbti.JI ub ~Toluidine blue technique U:!..:l)jJI 0.J) 4j'•a'•

1~~ ,·· ,,o .... SJ

~l:U.\1

- \J\l:JI :UC~I K l t · ~ .. •11 o..:l .ill U- '-'1 ~~ • Lli.JI -- .A.J '-l C" . u el .. ...r""' 3 _ ~ 3 _. .. c-- t:UJ .

L»l3~1 c) ~till\ ,~ •. o.:..l ~, ¥1.J~y.fi.l13 a/')··3 .-r.JI ..:llyJI uc ~~~WI

. (I 0-1) ~\_;C _jj_,sll .J~~ ~yJ\3 (2 , 1)

====~~ .... J\_,..Ji CF- .,;yo:Jl ~ l..;."W :i....IJJ ll===== wl..i.U · .n ~~ wutslll K. lutei ~ , •.11 oj Jll ~i Jc.LSJ ~ .J~ " - ~ ~ ~~ .J ~ . :( 1) J.J~

wi.J¥> ~ fli.J

~l..;¥~ftll ~l.l.U .):Ill - .J .

· .) _,l jlll • ·I (.):! '-'.).) . u~c....i.';.\S u....:;. - . .1.14-:U - J:U .. "'L.ac\J~

'OJJ.lll

~~ ~\J ~\ :l.Ji.J J":!))l ~ (-) oJ JJ.....JI :l.Ji. .)A (~ ~\ J J":!j)\ 4.ihi. ~(++++) ~I

orthochromatic 'OJ J.lll ~~~~~\J "'Lac~\ .} (+) ' ~~~

~\ :i..J~ . (-)' 4...Llllll .. ~ ..

;i,__.~~ p. metachromasia(+) oJJ.l....JI :l.Ji. J.o1::.} ++++ :i.___J,;J\ , ~

~~ ~ (-) ,~1 ~ ~ o.)J.lll ~~~~~\J 4.!!•0 .I\

i.)":!j)\ ~ (:~} 'OJJ.ll\ jA p,~\ cytons

'OJJJ.....JI :l.Ji. ~ (-) 'J":!))l ~(~ (~) 'i.)":!j~l -)(+) ~ ~~ J oJ J.lll :l.Ji. j.a( +) ~~

orthochromatic 'OJ_,.lll ~4-l ~~J ~\ J J":!j)\ ~ 0-o .)~ ~ l.>--"(~)' ~\ ~I

~~~~ ~I ~~ ~\ J ~llll\ "'Lac ~I

~I ~~ .)1 J ~I :l.Ji. 0-o (_) ~\ J ;j___.Ji. jA (_) .~L ~I .. J . ~l.l! ~(++++) L:.J)l.;.., .J\

~~~~ () .. ~,:i..J~ -~ . .. ~_,hll

(_) (_) (+) ~jly,~l :;\.lil\

f"' .,.II ~Ji. ~ (+++) J ;i.__.kJ\ ~(+++) (++++) ~\ J.kll

L.Jill .ili:. (++) 3 a-metachromasia p,)ll 6illl .ili:. (++)

~\· -~, JA~ ~\· I.)A

;i,__.~ ~ (++++), J";!j)\ ~ (~) (~) (++++) t:: -;I\

I\ ~_?.JI

L.Jill .ili:. (++) 3 a-metachromasia

~\ · ·~I JA~

73

==========~~-----~-~~_,_.c_~_._-_~ ____ J_~ ___ i_~_~~_··_~ __ ~_·. _~ __ ~]~======== wl:W .. II~~ I.:)Jt.U\1 :lJ ·'ill :lJ ··~1 :lJ "ts:JI ~L..ac.\'1 1-\.S:j : (2) JJh. ~ J~ ~ . • ~ • ../'"' J ._JJJ ~Y l>-'

wl .J¥t> y, .fiJI J

wiJ~~,;S.ll ul..l.i:i .,;.JI ~ J . ~.ft~'-'--~Ju~' r-~~1

0,!J _,J jlll J.Jjl . .J4.. u.tts u .. UJ..:;, . .lJ~ .. -uu ..

(++++) (++) (++++) ~I

(++++) (+) (++++) r--)' {_)o)~J a-metachromasia(+) (+) (+) ~I

orthochromatic

a-metachromasia • ( ++++) (+) (++++) ~I

a-metachromasia(++++) (++) (++++) ~I

P-metachromasia( +) (_~) (++) ~~,~~~ . J ..

P-metachromasia( +) (~) (++++) ~l.l\1 ~.,.WI ~_p.JI

a-metachromasia(++) (+) (++++) . h:JI .y> ..

P-metachromasia( +) (~) (++++) ;iJj\.il\ oUill

P-metachromasia( +) (~) (++) J9\.ll\ role _,JI

(_) dJ6. ~ w~ _,..uJI ¥1~~, ~~ _,..uJI ~ ~j3 JcLS:JI ~~I u1.S * ~3 a -metachromasia w~ lS...?~I ~I J,:..lyJI ~La) 'o11hochromatic

.~~ ~.JA Jew:+++'++++ J~ L.....i.::o. • L W . + + . . .. .JA~ .

. ~.JA Jew:+ , ~ .. .....;,Jew:+ . ~L..... Jew: -

wlu.S j · ~ - · j,J' · ·.·. ~ 1~1 ~ Ul:JI ~I .111 'tu w .. 1:..\ • y,.3 LP • tJ:.J -~ • r • .J ~ ,ft-=

(1 o.J_,...d1 3 1 J3~1) K. lutei ~I.J.lll ¥ oj3.lll ~~ ~ :ah-,3~1 jlyJI u-a o~3 ~ ~

~ J.l......;! ~ cytons ~I l_;)l:JI r-413 #I~~~~ 4-;:..y JcLS:JI u1.S3 '

~ · ~L..JI w~l .111 'tu · ..... :a..k)WI o~ , I·.;. • • .. 11 IWI · .. < ·'·'I - u---a . .) ~ ~ l,}9J-l 3 ~ LJ::U 3~ (.,?' ~ ..)-'-'

~ ·'·'I . _ ~ Ul:tll · .~ .111 :a..ub . ~ o - -L - wl:W ,,.. ~~ · 1 j,J.lll ..u6.. ~ 1 • 1 . - ..)-'-' ~ - u- . '-:? .)~ - 3Y. .s- . - u . - . ~

~~ ~ (39 ' 18) ~I l_;)WI r~l ~ ~~3 ' (38 -36) laj)4ll ~L.:;.i.l ~.111

~4 ~~~~~ wbl~'il ~l o.Jy,c-3 ~3y,ll ~~ ~ L;)WI o~ .J3j ~l

t} f'+A .J3j 3j 3 ~~~#I k; liu ~.JWI FJI ~~ ~ ~~ r~~L..JI

~ . 74 ~

=====~ .... J!_,..l\ <F- "p ~ ~ l....!_;J ll===== l>--L ~, (40)' o1.:o.Jl ~~I wi.Jl...a,JI L;A ~ ~ , ~~I wt._Jltill

4...W~ -~'1 UWl ~··\II Ultill .a..b~ :i..u.U ··'I ..)\- -11 ~I :i...JiLJI , .J o ..) • ..?' • .Y t • Y. •• J.Y." Y"" u.a J • '-:!'-- .JJ

~I ~ o~~l 4 _,.JI W)k.\..Ull U) ~ . ( 41) ~ll.JI ~~I J brush border

~ ~ ~ _,11 tA .JJ.) J ~ A /'J'J~I ..)\ _,.JI o.) J ~ J.)J ~ _»JI ~I J A..J }:JI

I".''" ., 4-:l}:JI ~I ~ ~ w~~l ~ .)~\ ~I j~l U) jj ,~\

, w~ J;:JI A....J;.j\ ~ 41·.;,_,~1 .)\_,.JI .JJ.) .clJjS_, ,(43, 42) ~..b....ll w~I.J.l\1 a.:j_fij

.(47 -44, 18) · "L.JI · ~ LJI 'tu 4.lli.c 'till\ o~ w~L:.. ~ ~- ~ t:" ~ .

~ - · .l.ll . ·. ·. ~ L ·.)lc lli ~I .1\1 .lJ§ ;; ..) .ill 4...J ~ ts::JI ~ L....ac. ~I · Gu La\ • U:U .. ~ • t:" '+' J .) • J .. Y U . ,

~J ,;;~~1_, 4.,.JI u)lci..Ull ()A~~ wy~ w.l;l ~, (2 ;;.)_y-aJI_, 2 J_,~l)

yl .)~1 L....£ UjJ _,~1..; ~I ~I ¥:ib t:" !..? ~ ~.).a wl j ~_f. ~J ..) Y-:! .clJj

..b~l ~J .clJj <.SY-:J ;;~_, w~ wu_u_,~l .)->?-J ~ ~).! L..a ,(48) Malcolm

..L.k. ~L....ac. ~I <2.1.\:j ~ ·_I::·. ~ 4.....a.JI · .. ..11 ..) · ~ "':II · ':II ~ r.r .. J l.»l J.Y." .JJ LF ~y' J ~

, .... -'1 . L..::.. <~...:... .L ·- .. 11 · · ~I L I ·-'1 _L·. 4...J ~ts::JI w~l '--~·-~ (.J ~J~ ~J ..... (.J."'-~ .)~J .Y • ~

-so, 49, 47, 46 d8) ~~I L;A ~~I ~J J...a_jj W ~Lh.a wl:-.~':11 o~ w~t.:..

.( 52

2 J_,.L..;JI) u.J--4a ~IS ~ ~ ~1.).111 ¥ o.) _,.111 ~~ Jc. lli uc LaJ

4J:·a ,JI y)l:JI r41J ~I~~ ~YJ 4Y wy~ w.l;l ~, (3o.J_y-aJI_,

~ u)lclli (5 , 4 · LJ - -'1) 4...J ''ts::JI ~L....ac.~l w .. 1.:.\ · . ~, .. <-. · .. -'1 - ... :.11 .. Y u .J~ .. Y ~ L»="- r.r ~ ~ e:_~ J

~ ~ ~ .JJ.) .U ufi.: ~ t?jjl ~.J.J:!tJI ~':II ~I..:JI ~~I ~I ~ J~ L..a

.(53) Hayunga ~I ). .• J La t:" ~I o~ 0W ,.dJLtiJI ~L....ac.~l o~

,.:.(;¥ .J:.fi.JI

ul )Jr 1 c:: .).lj ;; ..JA l.l; uP _,1 ..;JI ~.JJ A J"·a·· rl..b..:i.....l .ili:. 4-:li..:JI ~1.).111 ~tu w ~1

;;.J~I_, 2 d u':l _,~I) (~~I_, '-:?:- ... a··;ll u_,lll) metachromasia (~ ~J a I.Sli)

4 o.)3 .LJI ~~1_, ~1 ~ ;;~~_, ~Y W)k.lli (j_,..b,. ~ ;;..JAI...l;JI o~ uH6

~l:uJI o.)~\ wL .c:. . 'I · ·- 11 JS..:;JI 1 -'·I • 1 11 · •· L..a , ~~ .111 • -~ u-a ~ '+' ~ (.,S", cy..o Y. ~ .)

~ ( 58-55 , 18) u~L.JI u_,h~l ~J J...a_jj La ~Y ~till\ o~ uJ .(54) ~L:JI

JS ~ ~ 4YJ ~_»JI ~I~ 4Y w)lclli ( 7 ;;.J_y-aJI) ~j)l ~~J

Poddubnaya ~\.)..) t:" ~I o~ Willi, ~I¥~_, ~I ~I~ L;A

j~i ~I .clJj ~ ~.J.J ~~ W)k.lli o.)3 .1\l ~~I~~, ( 13 ) et al

75 )

========9~~---~-·~-~1_r_~ ___ ~ ___ J_~ ___ i_~-~-~--~_ .. _' --~JF=======

~j ~ j~ ~ ,(7 , 6 0\:j.J_,.....,.ll) ~_,hll ~ ~L...JI Jcu:JI uc lAJ ·.J_,b:J1 3

o~ ~ j y:. 3 uJ , ~ ~ c) tfA .J.Jj ·S)i S)j:; ':1 ~~~ w~_?.JI o~ ufi ~1

' ~~ 14\ ~')/1 w~h_qJI ~J jY-J ~.J (6 o.J_,.....,.ll) ~__».JI ~~ c) w~_?.JI

. (56) ~j_jj.J A.._ui.J~Y..fi.ll ji_,.Jl turnover J_p..::JI r.Y.fo ~d..L"j ~.J W.JA ~ jJ

4.9Lhll (.)'ll...}:/1 .J~I ~ tJL:11 3 ~...r.JI .J_,hll c) A.._ui.J~Y.ftll jlyJI .)_y:-3 uJ

u1..,. ... <}:11 ~~c) :;~ljl.a.l1 3 ~l:ll ~~ ul~.ill ~~~ w~h_qJI c) ~.J3~1

:;.JL__..;,"/1 ~.J ·' AJ·;.,JI ~lit-3 ~\ ~ A.._ui.J~y,fll ji_,..JI tS~ c))~ lAS, (59)

'-"···G....o 1.1< •-. U)lhll ~I l~ J;.9l.:.....J jl wlili.J · •11 ~ .. c::·. · .. -'1 - .... ·,\1 j 'I '-6--'-' .. .J ~ .J .. . ~ .. ' .. ..~ '-:? ~ ~ e:..-::-- .).J (S',

· l.lJ · . ·- .. 11 w~L:.. -L·· . . _q '--- 1 j y" ··.11 )I :V,)Lb ~ ~ ~jS , lY' .. .)A .J ~ .).J=-J '-:? ~ .).J lj y l..ff""" r.::.. .. .. '-:?

. ..i..L,:j · I · .c:: - · ~I ~'JI - · ... ·,II I~ \..GL:.. , ;,\",.;. J . ·~.~~I l.....l..lS .-.'I · Gu wL...I ~I ~ 1..), ~ .. • • .J e:..-::-- .. r..;r.- •.• ? 1..) • .)

0----.a .L-;.11.ll ~ I.J---?"-) d_Jj uc y:j y lA I jj , 4)GJI ~ Ujlb 3 ~ uc wlA~ G -·- 11 jl- -11 w~ ··-'I · tAl .. · 1 -"·'-\....., ~~I :V,h.uSJI wL...I ~~ .. ~ _,..,., .J .. ~ lY' ~ LP ~ Y. .. . .. .. .. .. .J

~~ ~ uL...I.J~ ~L&w.l3, ~I~ c)~ ~_,JI .J.J~I ~ ~ly-a'J~ ~3 ~-~1 jlyJI o~ .J:!.lli c) ~6..11 wl~l ~ w~~ ~~I ~~I jlyJI , a\",.;. ,J

'~I - ···- -'1 ~ wlili..J · •.11 · \.ij 'I (30 d 8) wL...I ~I · u ~i ~ , c.F .. .. '..5~ r..r- .... ?"' ~. I..S", .) ~ .)

. , AJ· ;.,JI ~ _,:J ~ lj~l

G •\.S.:l.\1 ~~~~ . _i!:;.lJ~ ~ ~L_g:j ._,: · · 'tull ~ .. Y r..,r- .. .J .. .JA .J..J&-= UC ~

~c) 4-S~IJ ~I.J¥Y..fi.ll jlyJI .JJj ~ k.a, (10-8 .Jy.a.JIJ 2 J3~1)

1·-'1 w ~i ~ , ')WI~~ ·\..:l:U "~'-'1 ~ 1 <". ···' wllkJI o~ t:=""..r .) J ~ .. . ~ ~ J ~ . ~ ..

r--AIJ w~y:;JI c) ~3_p...JI A.._ui.J~y,fll jlyJI rAi ~fo.~l ufi ~J ~I

4L93 A.:4b -¥:-JI ~ oj y,. yJI ~.foJI ~ 3 y,ll jl yJI ~ 3 lAS , ( 60) 4.Slh11 .Jjl......::..a

G.liW. ____g =< -- - 11 w~ ··-'I · · .c:: -'<II .kl ,(13) wl.J .-.:~n ~u.JI 4..J~':II ..h..O .. c.,r-~ -~ lY' .)y~ .. .J -~ .. . .

·l......a.i:JI ·I , (61) '- .;, ~~ "·'I ~':II wllkJI ~ ~l.SJI :ij\..h11 . \:u')l wlili.J · •.11 u 1..) .J ~ l..ff""" ~ .. .. I..? ~ ' .. -~

ji_,_...JI ~ ~ ~~ ~ u4-b:i_y;JI :;~ ~ ~b .U' aJ.;.,.,II ~lie c) ~6..11

'-:? 1;9611 _,..uj :ijk.J ~ ~ _;P o.J~ yUj jj ~I ~tu"j 4..;9lS.ll ¥1.J¥y,.fi.ll

~ JLJj ~ ~je u.a ~~~ ~4411 w~_?.JI .)1 _jj ui J_,:qll ~ 3 . ( 62)

'I · -~ "·'I '-·~lwL'-"·""t~ ~~~~ ·l.lJ~I · ·')II~ ~. (.5_)1-J l..ff""" .J '+:' .. .. . ~.J .. .. 1..) .. c..r ~ ... ~J,' a1.;.,.JI t~.J ~~I~~~ ~13 ~I~ ~I w~y~l .)#!

~ - 76 ~

=====~ 00 00 J\_,..ll (.;>- <ij>olll ~ ~~ :t....\JJ ]I===== L;~l 1.:A~y,JI o~ J_p,.J u\..g I~ , ~I ~i ~) 1. •1. ;,JI w~ u-a ~ ~ ~)

• ,I. ·.L::•. •,\1 :cu.JI _g ~~ .~1 .I.:. · r, .~\J; ~ wl..J.Jihll tl ·i ' _g "il ~ ~ <.,?' •. c...r • _, y=> l)A ..r • -- .J-l ~ c...r ~J '

- -Longitudinal Muscles LM4.;hll~l

Mesenchyma tissue M~_1L.JI~I

Vetellaria gland v ~I Jill\

Testis Te~l

Cytones C~l~l~

Tegument T~l

Internal seminal vesicle lv ~IJ.ll ~~I ~~I

Ovary 0~1

Cirrus pouch Cp ~lj:UI ~

Eggs Eua~l

Uterus U~)l

Scoles S~.J)I

Neck N~l

Uterus branches Ub ~)I u~ _):JI

Ejaculatory duct Ed UjWI ;Will

77

'LM ~_,.bjl u)l...a.JI tY.HK. leutei o.)_,..ill ~P ~: (1) ;;.Jy.aJI

'C ~~ ~~ ~' Te ~~ 'V ~~ j.h.JI, M ~_»JI ~~ (4ox) ... <·'.II .. .. . ,. . I.;·. .. ..... T .llb....ll . ~ o~' ~ l.>:U~T-'-''\l-W' ...

. .

< ~ ... ! .

: .. :• . . 0 . ~ . -

• ,. ~ 'tl .

• , , • • .;:~ • : • 9 I

-~ ... , •• t '. ~; .-a.· • ...:.rt ., J~

•• ,J .

~ . 78 ~

==========~r-------J'-~--~-~--~_J_,_;~-~~_~J_?_Y_·u_~_~·-~-~--~ __ 'J_J~JF=======

'Te ~I' V ~I ..l..l.i..ll ~K. /eutei o..l_j.ill ~JC cJili: (3) o_Jy.all

C ~~~I~' T ~' LM ~_,.hjl u~l, M ~__»JI ~~ ( 1 0) ... (·".II;; .... I.- ~\.S :i._Wj'

I X ~ ~L)~ ••

Cp

~\.S ~' Cp ~1_:,~1 ~ ~K. leutei o..l_j.ill ~JC cJili: (4) ;;_)y.aJI

.( 1 OX) y.,&ll ;; _; 0~

~ - 79 ~

========~~~----~-~-~ _c_~ ___ ~ ___ J_~_. __ i _~_~~-- -~-~_·._ .. __ ~]F========

...... L. ' ''I.S 4...ll9J E . ·- .. II . K l . .. .ill . • L'~ A (5) .. . -'1 o_JS U-"'*"" l...i....l .. ' ~ L..>:!+.l . eutet O.:lj ~.JC c:-""""""": O.J~

.(40X) ~I

'U r")l ~K. leutei o.:J_,.ill ~.JC ~: (6) ·o.Jy..:JI ~' LM :i.:J_,.bJI u~l 'V ~I .:J.:JiJI, Te ~I, M ~..»JI ~~

.(40X) ~I o_,! 0.P);JI 0.JJ

~ . 80 ~

81

s

,Q_)_)'I ~ 'N ·:··-'1 s ·)I . K l ... .ill I .L. L·~~ (7) .. - -'1 '-' _ lY"-' LY':l.J lJ:!+.! . e ut e 1 o ..l .J r.r ~ ~ : o _) y-=

(loX) ... <·"·'1;; ... ..l _t _·:II . ~ ~l.);!yy

~~' Ub ~)I ul.!:.fil ~K. leutei o..l.J.ill ~y:. ~ :(8) ;;_)y-aJI

.(40X) ~~ o_} 0.P)jill Jjjl ~'Ed :iJjWI ollill' E

========~~~----~-~-'_c_~ ___ ~ ___ J_~ ___ i _~_~~_.·_~_'_;._, __ ~]~========

~)I uiL .foll '0 ~IL.»H K. leutei o.)_,.ill ~y:-~ :(9) O.J~I (4ox) ... <·:11 o .<i · .) ·1 --·11 .. ·1 ~, E ··- .. tl, Ub . ~ ..r l);! y_r l,j.).) .. ~

~.J} ~' E ~~ ~K. leutei o.)_,.ill ~y:-~ :(10) o.Jy--:JI .(40X) ~I oy! 0,!.)_,ljlll

~ - 82 ~

========~~ _____ .. _· _·_JI~_. _'_~_· __ ~_J,_'_"_A_J?_J_"u_·~-~-·~-~--~ __ IJ_J~lF======= References 1-Scholz ,T. ;Shimazu,T.;Olson,P.and Nagasawa, K. Folia

Parasitol.,48:275-288(200 1 ). 2-Richard,S.K. and Arme C. J. Parasitol.,68:425-432,(1982). 3-Richard,S.K. and Anne C. Int. J. Parasitol., 11: 369-375,( 1981 ). 4-Moravec, F. Folia Parasitol.(Praha),31 :373-374,(1984). 5-Scholz,T. Folia Parasitol.(Praha),34: 128-130( 1987). 6-Chakravarty, R. and Tandon. V. Proceeding of Indian Academy of

Sciences, 98(2):127-132,(1989). 7-Scholz,T. Folia Parasitol.(Praha), 40:99-1 03(1993 ). 8-Molnar, K. ;Majoros.G.;Csaba,G. and Szekely,C. Acta

Parasitol. ,48(3) :222-228 ,(2003 ). 9-Morley,N.J. and Hoole, D. Diseases of Aquatic Organisms,23 :93-

99,(1995). 1 0-Chubb,J.C. ;Eiras,J.C. and Saraiva, A. Folia Parasitol., 44:131-

138,(1997). 11-Lindholm,A.M.;Reuter,M. and Gustafsson,M.K.S. Parasitol. 117:283-

292,( 1998). 12-Whittington,I.D. and Cribb,B.N. Adv. Parasitol.,48:101- 223,(2001). 13-Poddubnaya,L.G.;Mackiewicz, J.S. and Kuperman, B. I. Folia

Parasitol., 50:275-292,(2003 ). 14-Zdarska, Z. and Nebesarova, J. Folia Parasitil., 53 :73-75,(2006). 15-Fattohy, Z. I. m. Sc. Thesis, college of Science ,Universitry of Mosul

(1975 ).

~~ , r _,h.JI ~ , o4=JI r _,k. , ~LA ~t......, .J . ~ Y-ii U'"~ , .!J)I.SJI -16

. ( 1992) J..,ayJI

,25 ~1, ( ~Lp.JI_, ~~ r_,l,JI ) ul.....,I.JJ ~ .~ Y-ii U'"~ , ~)I.SJI -17

.(1998) 163-157 :2 J.h..ll

~~ ' r_,l,JI ~ , o4=JI r_,k. , oi.J..¢J ~_,_;bi . ~ .J:!ii U'"~ , ~)I.SJi-18

.( 2001)' J..,ayJI

19-Al-Kllak, S.N.H. and Rahemo, Z. I.F. Rivista di Parasitol.,20(64), N. 1- Aprile 45-52(2003).

r_,k. . J;_,h..a <.S~ , ~1_, ~La....J )y.J , ~t.;~ ~ .J:!ii U""~ , .!l)I.SJi -20

(2004) , 86 -78: o4=JI r~ ~G. &1)1 J.h..ll , ( 15) ~~ 0.!~1)1

.(1995) ~yJI ~~, r_,h.JI ~ ,oi.J..¢J 4..:.._,_;bi .~1 y~, ~ -21

22-Mohammad, S.M. ;Al-Kallak, S.N.H. and Rahemo, Z.I.F .. Rivista di Parasitol. 17( 61 ):54-52(2000).

83

j

========~~~---~-·~_~'_c_~---~---)-~ ___ i ._~_~_~_-_J._i"_~ --~JF======= ~~ , r-#1 ~ , o~l r-_,lc' ~~ AJ~.J . ~ ~ 1..5~ ,~i -23

.(1997)~_,.Jl

~~ , r-#1 ~ , olp.Jl r-_,lc , ~~ AJ~.J .ytA_,ll -¥-~~ 'i.S.J.iJI-24

.(1996)~_,.Jl

25-Rahemo, Z. I. F. Iraqi J. Bioi. Sci. ,15: 42-52 (1996).

~I' LJ:!..lSI)l ~I.Jj ~. 1.....9.j\..j ul..;L, ~ .J <rfo r,:Aly,J ~j, ~.J -26

. ( 1991) 14-9 :(3) j.k\1 23

27-Rahemo, Z. I. F. and Al-Kallak ,S.N. H. Acta Parasitol. Turcica, 17(2):74-78 (1993).

28-Rahemo, Z. i. F. and Al-Kallak ,S.N. H. Acta Parasitol. Turcica, 22(3): 330- 333, (1998).

~_,.Jl ~~ ' r-#1 ~ ' o~l ('"_,lc ,ol.Jfoj ~.Jpi . r-jb c:-1~ ' .btp..Jl-29

.( 2006)

~~ ' r-#1 ~ ' o~l ('"_,lc ,ol.Jfoj ~.Jpi . ~ ~l.b ~ ' ~1-30

. ( 2006) ~_,.Jl

~4- , r-#1 ~ , o~l r-_,lc , ~La AJ~.J . ~ ~\.! ~~ , ~~1-31

·( 1999) ~_,.Jl

, <"..,WI ~ , o~l r-_,lc ,ol.Jfoj ~.J_;bi . c:.ftJI -¥- LJ:!..lll C)L...a ~ , ~-32

. (2002) ~_,.Jl ~~

. (.J":UjJ 0~~, ~.J ~tA ~\.;.. :i~ ' (,?.J~l ! ~ y,ii U'l~ ' ~~i-33

·( 2006) ,64-52 : (2) j.k\1 '( 18) ~~ ~~.J ~_;Jl

~~ ' r-#1 ~ ' olp.Jl ('_,lc ,ol.Jfoj d...:...Jpi . * ~~ ' -,?j.Jjl.J.ll-34

.(2006)~_,.Jl

35-Pearse, A.G.E. "Histochemistry: Theoretical and Applied. 3ed. Vol. 1, Churchill Livingstone, London, ( 1968).

36-Smyth, J.D. and McManus, D.P. "The Physiology AND Biochemistry of Cestodes" .Cambirdge University Press, pp: 1-22, 60- 62, 114- 130, (1989).

3 7 -Mader, S. "Biology 11 Mcmgraw Hill. Companres. ( 1998). 38-Lee, D.L. in Advances in Parasitology , Edited by Dawes, 4: 187-

214,( 1966). 39-Lumsden, R.D. Exp. Parasitol., 37: 267-239,(1975). 40-Barrett.J. "Biochemistry of Parasitic Helminthes" the Scientific and

Medical Division Mcmillian Publisher Ltd. London and Basingstoke. Pp 380,(1981).

84

====~~ .... •l_,..ll tF- <iP ~ :i.;."t;..;s 4....1.;• ]I===== 41-Lawton, P. ;Hemphill, A. ;Depllazes, P. ;Gottstien, B. and Sarciron,

M. E. Exp. Parasitol., 87: 142-144,(1997). 42- Rego, A.A. Revta Brasiel., 12( 4 ):791-814,(1995). 43-Scholz, T. and Hanzelova, V. The J. of Parasitol., 85(1): 150-

154,( 1999). 44-Gupta ,N.K. and Kapoor,M. Rivista di Parasitol.15( 1/2):63-79 (1979). 45-Hayunga,E.G. ND Mackiewicz,J.S. Canadian J. Zoo!.,: 790-

803 ,( 1988). 46-Mansour, M.A.;Kelada,E.P.;Kalil,A.I. and Aboulaban,A.M. Bulletin

of the Faculty of Science Zzgazige University 19( 1 ): 300-321 ,( 1997).

4 7-Waitz, J.A. and Schardein, J.L. The J. of Parasitol. 50(2):271-277, (1964).

48-Malcolm, K.J.Inter. J. for Parasitol. 28:913-223,(1998). 49-Conn, D.B. and Etges, F. Zietschrift fur Parasitenkunde, 70: 769-

779,(1984). 50-Muthakrishnan, S. Acta Histoch., Bd. 50, S. : 174 180, (1974 ). 51-Arlene, J. J. of I-Ielminthol., 94: 251- 261 ,(1975). 52-Muthakrishnan, S. Acta Histoch. ,54,S.: 1-4 ,(1975 ). 53-Hayunga,E.G. Proceeding of Helminthological Society of

Washington, 46(2): 171-179,(1979). 54-Dunn,T. S. ;Nizami, W.A. and Hanna, R.E .. j. of Helminthol., 59: 1-

18,(1985). 55-Rothman , A.H. and Elder, J.E. Comp. Bioch. and Physiol., 33(4):

745-762,(1970). 56-Sharma, P.N. Indian J. ofExp. Bioi., 17: 479-483, (1979). 57-Mansour, M.A.;Kelada,E.P.;Kalil,A.I. and Abou Laban

A.M.Proceeding of the Zoological Soceity Arab Republic of the Egypt, 27: 81-102,(1996).

58-Chandra, K.J.;Hanumantha,K.R. and Shyamasundari, K. Proceeding of Indian Academy of Sciences, 94(1 ): 11-19,(1985).

59-Lucas, M. S. ; Felleisen, R.S. ;Hemphill, A. ; Wilson, W. and Gottstein, B. Mol. and Bioch. Parasitol.,91 :281-293,( 1998).

60-Chakravarty, R. and Tandon. V. Helminthol., 26: 259-272,(1989). 61-Roberts, L. and Janovy, J. " Foundation of Parasitology" McGraw

Hill. Higher, Education, 7th ,(2005). 62-Cheng, T.C. " General Parasitology" . Academic Press. Inc. Orland,

Florida and London, pp 387-444, (1986).

85

I

I: