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Transcript of ‰¾¿™167 CagA qr+,a—À`fcd ´ˆ˜À¯)+,*kl

Page 1: ‰¾¿™167 CagA qr+,a—À`fcd ´ˆ˜À¯)+,*kl

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狀狅狏犻狉狌狊

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【犃犫狊狋狉犪犮狋】 犗犫犼犲犮狋犻狏犲狊 ToinvestigatetheeffectofCagAoverexpressionontheexpressionofglycolyticenzymesin

primarygastriccancercells. 犕犲狋犺狅犱狊 TherecombinantadenovirusvectorpAdenoMCMVCagA3FlagIRES2EGFP

(pAdenoCagA)wasconstructedandtransfectedintoHEK293cellstopackageadenovirus.Thisadenovirusand犎犲犾犻犮狅

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rygastriccancercellsfor72and24hoursatamultiplicityofinfection(MOI)of50.TheexpressionofCagA,hexokinase

2(HK2),glucosetransporter1(GLUT1),enolase1(ENO1),pyruvatekinase1/2(PKM1/2),andlactatedehydrogen

aseA(LDHA)proteinwereevaluatedusingWesternblotanalysis,andthecellcycleofinfectedcellswasdetectedusing

flowcytometry. 犚犲狊狌犾狋狊 TherecombinantvectorpAdenoCagAwassuccessfullyconstructed.Comparedtothecellsin

fectedwiththeemptyvectororcellsnotinfectedwith犎.狆狔犾狅狉犻,expressionofHK2,GLUT1,andENO1proteinwas

significantlydownregulated(犘<0.05)inprimarygastriccancercellsinfectedwithpAdenoCagAor犎.狆狔犾狅狉犻GZ7.

ThepercentageofcellsintheG2/MphaseincreasedsignificantlyingastriccancerSGC7901cellsinfectedwithpAdeno

CagAfor24,48,or72h(犘<0.05).TheratioofcellsintheG2/Mphasewas16.90%intheemptyvectorgroupand

65.93%inthepAdenoCagAgroupafter72h. 犆狅狀犮犾狌狊犻狅狀 犎.狆狔犾狅狉犻inducedthedownregulatedexpressionofglyco

lyticenzymesbyCagAinprimarygastriccancercells.ThisactionmayberelatedtoDNAdamagethatarrestscellsinthe

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Page 2: ‰¾¿™167 CagA qr+,a—À`fcd ´ˆ˜À¯)+,*kl

G2/Mphase.

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Page 3: ‰¾¿™167 CagA qr+,a—À`fcd ´ˆ˜À¯)+,*kl

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Nov.2019, Vol.14,No.11