r:t;: . :: r:;,.',.,.;,1: l',qi,t rl;'-ril.jt:lrt:ia

l:.:,,Ufllft.ffg0:

26 rr:ar:fisrtrilvrunrcn{ uri. ilfi t+ qfufi t il:vqilisrnr:finruvtnunrofl{ il:l, pri'rfi s :.nv,itii zs+z

n'r r H fi n dl rdo u'ri uq5'rql ailri durf;i a,rlil u

Frozen Semen Production of Thai lndigenous Cocks

aun: surviu' rilaqd'nd tru6u6o:' nnnin: InBnfl:*g' rvriuvli r.::$n:vall'

SuntronSunathair TerdsakCheunchomjitr2 Kulapath Phothikanit2 Thevin Vongphalab3

nr:fin:srzuoro.rnr:6q;rrfi'o'lrifiurfia.rtyru + fufiodori'ulu t #ljorv{ siorjsrurruto.rY X i i < i o t4o'

u1[To ntrrfldou?lllo.roa6 rj3rurrur-irrfiorrriufi.rffzu6n'Lri'oiooireia{u unvzuoto{on:rnr:zueilL6elt

? x u,! a vi a,Y&,eidatodilorurtfiotrritr{.:fr:yu:rrnrnr:rfllfnurironqir.:nu Lounrnr:5ourt$o LnnuLxJ0.l [Ylu01LJ 1 !siruru 1o oir zunnr:6n:grlnrl'ir rlSrurrxt*rriYrtiSf,rfir{ufi t, 2, 3 trav + firj3rurr*ladl 0.30,

J i u 'e v Y & Ao a - A

0.36, 0.28 ttns 0.27 elnnnn: ql3J0't9l1J nr:ternouvrttlJl"J9rT,l LlJl'1.:141'[11J0{U'][50?1:OLnr']1.111Y XAa d -A1, 2, 3 rLny 4 rilfiu 64.75, 76.55, 59.25 unv 46.25 ql']Lj6i'loiL urrfrofrSorflliufi Z

rr.loirfiuai'nr:rofioufirartir16'ouriufru#rnr:nvn'ruR.ln'i1fi3orffliufi3nnr+arirtfiriuairfirynr.:aff6 (p<0.05) nr:zu6qrfird'ouriru{lrafiuqiooi':sioiuornurrfi'ofiie'ifiuiufi z

A Mt A ,- - a q< U'o j Y Xfl'u1r:nrufifl'Ld'rurnffqo (5"39 uaoo) fq:rnr:zuaru6ounvnr:flnoanto.l'Lriiltfralo.:urtfro

c

urdrr{.lfiufflin wi z4d'.lur'[riruqneir.tni!firfirinr*ruru 3 t6ou (az.so ttdr 46.66 rrlair6uoi',

34.37 $n; 36.36 r:Joir6uoi' otrruoiroYl)

u,A a M t d'aldlFltr : tnililuJ0i Lvru 1.[1 LT0 nll ttTlt"]J.]

^u*orJ., Thai indigenous, semen, frozen

' nnrfrid'ulr o ao! fruf,#er{uiirJ ri o.:.1 :rir a.liiil f e I z z o9q

Burirum Test and Research Station, Prakum District, Burirum, 31220.

' aufio-uurn:rirl.rniuri#o{nhnrprnor.t o.airnuB o.nrnrlS 1 51 9o

Lumpayaklang Animal Husbandry and Research Center, Lamsonli, Lopburi, 1 5 1 90

t nroitrao'leraqi oru:inr4e:eraoi tJuriflur#lloutrriu o.tfiol o.touuriu 40002

Department o,f Animal Science, Faculty o{ Agriculture, Khon Kaen University, Khon Kaen. 40002

KKU. Vet. J. Vol. 14 No. I 5'n KKU Veterinory Annuol Conference 2004, sth KVAC 2004 27

' . ..)a! \.

AbsJ act

':r:.].:.Oi:i ,

.';i'f.ll

The objective of this study is to determine the efJect of daily semen collection on the

volume, motility" production, and fertility of frozen semen. Ten Thai indigenous cocks were

continually collected semen for 4 days a week. Volume and percentage of motility of semen were

0.30, 0.36, 0.28 and O.27 ml;64.75,76.55, 59.25 and 46.25 collected on days 1,2,3and 4 respectively. After thawing, the day 2 semen was showed higher motility than the others

(p<0.05). Frozen semen production was highest on day 2 semen (S.SO straws per cock).

Fertility and hatchability of eggs from layers mated with frozen semen of Thai indigenous from the

24-hour and 3-month storage were not different. (51.50u",44.660/o and 34.37oto,36.360/o

respectively).

^ ? X , d u,3 anlrzuo aul rro rrTirfiir o.:"Lriff urfi orlr r rl:r rvr etr u #.J fi:r rrrunr:fin rErnr:zu6 oirir

a-,dvf,8d!luYXdqi-Qwdvr50tLTtt1j.tlj0{ LnililnJ0.J lylu1.[0u Yt.l1A01a3Jlruultnu']nt-JnrrLTfl1Tto3JlJ0.Jnun1:ttflno't1o.l

rtnii'oqitun:vtlxunl:Llliufr.r orn:ru{1ullo.r o6d'nd rrflvnilv (2545) d.rdn:gr:ro-rFr?1ilrdxJy I j q . :' X u ,X a 14 , 6 v w q wi

flu1J0.1nnt{0T8fl?1 tuaJ1rflil [u,n'l:?'t'tul[50 tnill,ttil4.] [?1 uLLTttlJ.] [ott1.[3u1']lqtxTL$ytT39lL

Fr?'rilrliildlA +-a lrJoir6uai-fioqr:rnr:tn6oufi:trqr-r:l:rrr{u 36 rrJo{r6uoi'uo:yfrnr:

zumrurfirruoi'ruoeivY.ruro 100 d'ruoi":zld's rnruirfifn:rnr:zuarfiorfir.r 16.39 ujoiiffuc,i' d.:q

floqnd'onnl!:'rEl.i'rl.rllO.r Graham et al. (1984) *nr Lake (1986) finr.l'jr ur16'ouriu{1fi% , ! - , Y X a,tlruurqiooo:1nrrzufl:Jdqqirun.:nr:n1nrn1u rLei Buss (1993) Truvruilillrfrafrrirutlxuntrrudufi.:Iould'n6rqo:an rilusr:flarrYunr:u{.rqr-rrarrrnei'osi t#oa6sfruru i so #ruoi':,, t Id'a

99

fn:rnr:zu aru 6fr o dlu::u'j 1t 7 -7 g u-Joir6uci' catvert ( 1 9 7 8 ) :r u.:runr:t#ar:nd rro:o n riJila

ar:flornlunr:ufr{e-rro.lrrynfoai fizunifirqianr:rJfrnuBrLaroni urnvfizunlunr:uJfiuuuiln.t:il

ir.rloroq6 uqid'ruunn6rto:ofl oonqrnaqAqryirl#fiq:rn1:r.rfiilfiofidu sexton (1g78) un;t - -t

Schramm (1976) rru.J'run1:uriirfilr.irrfiolriToul"frBeltsville poultry semen extender (BpSE)

irrun-l dimethyl sulfoxide (DMSO) :rd'r o-to r:-ia{t6uqi' nujrfid'qr:rn1rrifiil6oilryru'xu

50-G0 uloir6uci n1:rffu;nururrdoudufr.r 'lflun-rtuln:rournnr -t g6 o.lflrrfidLdufi

uru 2 iJ g i6au #o:rnr:zuerLfiohinonr Lake and Stewart (l au) :rrmu'jrflod'ufirfitrrio.ra Y d' , a A < - vlcjt e a v 6 ,u r

n:Jn'l:9.JfltJgl9r €t0 nnrn1flfl0.tu'tLt0tLTttlj.trtn:iTn1+1 L? tun{ ttflgi3tJuffl"l1lu51J 0.:ttil [nA-a?lTtnl:zuflNtruil

2B ?1rfl1r#n?[?iyrusran{ lr. flfi t+ ol"ufi r il:vsffisrnr:finrurnsnrcni ls. n*,:fi s :Jry'fiil 2s47

nr:fin:Ertuqr:ifiriJunr:6n:E'rr.rnro.:nr:iourr{Yo'[ridurfio.:tyru + fufioreioriuriJurrnr

t f;'rjorv{ qiorjsrLrrxr.roo*,dr nr:ro6oufiroron6 :J3rurrx#rrfi'oirriufi.:fi2fio'[oi'qioor-reioiu9

un; zu nr a.: fn:r nr:zu or fi tr t o #', rdr uri ufi r fi :v u y n nr nr:rfi rin :*r un n sir.r rTu dr o r iij ur T 5q ^ ? X t d vr ,3 a a - u ,& a q -Aa a

1J53 tUTl{lUnlTt{nOU'ttT0[tttLlj.]l10.r [n]rurilai nlTTnul tLflr?\qilu't LnfliluJa.l h4milflruR11^l9i

1'51"i'rj::I usrilun'r:in rcr fi'urn :: ri oi o'[:J

a-, ' ''.Aara c.r:;, ?crollag?fi?f,t, "" "" I I ".

o4df46{A?YlO n O0 ttn s tTJ [50%

' - a,X a at o

flaflur'lnniltfio{aru 1 il ohuril 10 a1-x rdrrnonnv t oi": unvurilrihinnzuarun]{nr:qq{

ri'rorr 8-9 16ou druru 20 sr-r rfi'u.ruun:roi':rrio.:nr t qir efrrffunr:y,r^oofisr*rii{'uunsqq

rir:niuf#o{eirlnrrurnor.l a.ilflu; u[rinenoryr".rvrrotci'irnr:rirunurG tvifnduflorriul:er

9qo9

firqrsrfinunsofirr(i' $nstd'lriililo-r t #ilorv{ riourdrvirnrrilono{ tv{otar:iunv z qi*rtu

oraurd'runvrfiu (orrnr:rirrSo;r-Jqrxqirnr.:lnrurnr:TrJ:6utilo'rn'jr 16,/, td'ffuil:srurr*gq

t z s n iru,ror-r uiu) urn ; fi rir m; or olri'ff uq a o o r? n1

o?j.rcaqYrnrt5oil'rr8a rrdufio n'lrdsalu nrrruaalrfi ssJ $aen1r$[n'hi

iorufi:lu1r{ioTouifinr:uler?r1'rrr'urEnlLiito.o Lake ( 1977) + iu6osion-uun;uqoll-n

s {ulu t #:Jorf tilutrnr t r6ou yirnr:o:ronrunrnTourrxyEr:roos€iud'rr#ot{fiaruranfi 41q9ts

o.:srrrnr6ua (eirxett, 1981) r6oor.rrirrfi'od'ru epsr (Sexton, 1g77)fifi oH,rso t o i:Jair6uei'qvdM,o,o,M"lri'fierrrrrrirLriu 1,22oxt ou qi'ru ffn6fiqr: irn;finr:rer6aufiru!Le]:llildrrfi#r \ilsirn'irzo t:Joirfiuqi'6.:orr.fulvfrriru6'aririufi.: Iou:r:tr"^rrri',,fi'.rt1.r'ro 0.25 fiofifiqr: neailunfiI a v? a Y < ct

t q q

[eunrrLd'uru{.] runvr.irufiurfJuoTrri':unqorunnfiiilu 4 otfl1rrnrfius tud'er:r t'czurfi rirt:J

a''ruu'[a'[ulqrr[01*r14fl? -2o"C u'ru 25 urfi unrfi -80"c z utfi riouudn.:tu'luTn:raurunr"

L i o q ? , i a d aaa- 1 96-C u1lA 24 tr Lrut yrlnr:nrnlu luuraunoruunlr 41 "C u'ru 20 Turn n:ronr:ilfrlst

AAurrnr uerfi o ufi ru:-rl n:.:'[:Jrir.rrarir ( eirret, 1 9 8 1 )

aa^?&,eidtn1:fln:912d1J 0{ A O:1n1:91flil AqlJ8{1{'t tT0 Ltgt[lJ,]?1Ti Uy[? d'rn"l:Inu:nu1tton911.t nu

T , V X ' a A< q U 5. @ e u I A u rM rtouflilu1[Totttut.:rufiutuluIEr:rourunlu'ru 1 fu uor 9O fu 1Uzuarurrurnurril [nnntJnr 10q9

qr-r qril?fi1o.: Lake (1977) Iouvirn'r:dr*rd'r o.zs finRfiorzld'a z sr*.rz#rJorri'virnr:rff:tVl , v A a d < <r oll , r q'[rjnn.:zuarutvtutJ 2 ?1,l tnrrnTurir.rrilu riluurnl 7 iu ur'[rirrirfln (Van and Leenstra, 1995)

vi 'i - A t d dun;virnr:eiot'Lriff i B {u rfranru:Joirfr'uoi'hrail6e} (,z,fertility) unyr:Jo{r6ud'nr:finoon

(,zohatchability) ufian:u 21 tu ('::inci, 2531 )

KKU, Vel. i. Vol. 14 No. I 5'n KKU Vetefinory Annuol Conference 2004, 5'h KVAC 2004

ad9aan1:? to:1 s14" o N dYtl$ 6{n 9l

a Xq-nr:finr*rfi"Ld'tizuurqno{rutt completely randomized design (CRD) Ioudn:crzuntor

a X ,A vr ,f a u , q q

nrrSqurrfiolat'lriffurruo'l t?ru 4 ?ufloaofiulu t #rJorvi qiorj3rurrutolrirrdo nlTrnfioufia a V X , < j a Mg,

1o{aqA rjirurrxurrfiouri*firff26o"[d'qiaqrirqio']u rnvr.Jnro.l:yuyrln'rfirfrlinr*rtululqrLauruo'lTAA^61[oui6irn:rrrisnrrurnJ:rJ:ru (analysis of variance) uorrooa!nxlrJunnsi-l.]so.lri'tril6soi'rui6

Duncan's new multiple range test (oiry, zs+o) eirufior:rntrzuflilAqro.:rirufi'ourirrfi.:fi:yu:d v olt a ta d ^dtxalnlrtfruinBruru z+ d'r-[ru.r rav 3 16ou r:Jirrrfiuuifr chi-square lould'I:J:*n:t

sirr5o:ri SAS (SAS, 1997)

r,t nt o.l nrrio* t#alrinlurfi ortr r + iu6 o qi o riulu 1 a-rj orvi oi o rjilr r*t o.rur rriolrl

durfior'[nu riouunyHn-.:n]rr6oo1n (taote r ) tunrirufiSourrdaiufi 1, z, 3 udy 4 fij

UTeJlilrilnurnlnu 0.30 t 0.1 '1, 0.36 + 0.1, 0.28 + O.O9,0.27 + 0.08 $ny 1.24 + O.47,'1.40 + 0.37, 1.18 + 0.36, 1.o7 + o.s finfffifl:n*ttdt alilnhei!':-uiorirrfi'ofiranqi1{rYtrZru,a,A

yr.r 4 nfltJ-lrulLzunn:;nr:qiailSrurrxra{il']rfroil{riouunrun.tntrr6oo't.l (p>0.05)

nr:ro6oufil:Jrirrurirloroa6riouririlnrsu?unl:uriufir (rante r) tunrirufi3ouru6'a

{u;d f ,2,3 unr 4 oyuyirniu 64.75 + 30.88, 76.55 + 23.5,59.25 + 23.3.1 uas

46.25 + 20.34 njoir6uoi' qril6iroiu nr:rer6oufib]dr.rrarirlo.:onAfiSerirrri'o{ufi 1 uni 2, * A ^^i^ , * ., ,,

t

stn'jrfufi + odr.lfiriueirri'tX (p<0.05) eiruoaafiiouru;i'ofufi s riu'lriuarnqir.:orniudu.l

(p>0.05)

zunornnr:uri*{.: (tante 1) nu'jr nr:rqrfioufi'l:Jrir.:Hrirlo.:oq6tunairufiiorirriio{ufiqi

1, 2, 3 uny 4 un-rnr:*riu{.:riJu 31.80 + 15.22,39.87 + 13.70, 29.32 + ,12.50 ttns

22.37 + 9.93 rrjoir6ur,i' ona.rnhoTu nr:rqrfioufilotoe6lunoirufi z m.ln'irnrialfi s uas 4cc{9

o rjrtfi riueirdry (pco. o 5 )

:J3rurrurir16'oudufi.:fizu6Ertoi'(raore r) nu.jrlunrlrufiiouirEioiufi r.2,3 uns 4^ 3g

nrilr:nilfiqurrfioirriufi.:'loi's.gz+ 1.81,5.39 t 1.51 ,4.61+ 1.32 r[d;4.50+ o.zo r:Joir6uoi'^ ? X , de iEnilairq! nr:atffourtfiaridufi.:"lunoirufi z o.:n'jrnciarfi s rns a odr.ifirilefrrirg (p<0.05)

29

v d c'; . '; v d Yd-

30 ?15d1rfinxrlililu61dni il1.ijfr 14 0fiJfr 1 il:yqilir1n1rd'A1uv{1iuf,1f,eli iltl. uton 5 tljttrt],g47

Table 1 Volume and motility of pre-frozen and post thawed and frozen of Thai indigenous

production which collected on date "l "t, 2no, 3'd and 4'n of week.

Volume(ml) Motitity (%) Frozen semenCollection date

pre-dilute diluted pre-frozen post thawed production

,SlI

2no

ordJ

4tn

0.30+0.11"

0.3610.10"

0.28+0.09"

0.27+0.08"

1.24+0.47^

1.40+0.37'

1.1 g+0.36"

1.07+0.35"

64.7 5130.88"

76.55+23.05"

59.25+23.31"b

46.25+2034b

31 .80+1 5.22"0

39.871'13.70"

29.32+12"50b

22.37+9.93b

5.23+1 .81'b

5.39+1 .51"

4.61+132b"

4.50+0.70'

"'o'" means in a column with no common superscript differ significantly (p<0.05)

rruyndrlroinl:rfiu;nu1 (Tabte z) nrl'jrrirrdofi6erfrliufi z zu6orilurirrfi'ot d ^

a dv ,4-- i u I e v dt

t[Ttt1j.] [[nyrnurn1!l1v11 24 Te.J. 1, 2 unr 3 16ou ilon:rnllLanoul']Lt!u'[il1]'1.114u1[:Ju 40.3,

40.0 rldy 35.5 rilo{rffud'; 39.9,40.3,40.0 $n3 35.5 uJoir6uoi'qril6ireiu drarn'lrto6u1t da . - : .---,1 -. - ' -.e^.y^-.

Y 3 q u tour{iluufl1flrUU.iy.l't.lRff6 (p<o.ot) airu:rurtrnrlunr:tff:iinrcrdr16o'Lu'Lu[Er:routnnlu-11{

ols a q , - Aa a Z X Z' - A A u re ez+ d'r]rur 1, 2 uds g 16ou 'luuoinyiufiSorfirurrSauuon:rnrrtflAouffut.Ll"L:Jrirlraur

trj ruErnqir.rrYu (p>o.o 5 )

Table 2 Motility of frozen semen on different duration of storage in Thai indigenous production

which collected on date 1", 2no, 3'd and 4th of week.

Motility (%)Collection date

24 ht. 1 month 2 month 3 month

1"'

znd

3'o

4'n

31.8 "

3g.g b

29.3 "

22.4 d

35.0 "

40.3 b

30.2 "

25.2 d

32.2 ^

4O.O b

30.0 "

25.0 d

30.5'35.5 b

25.5 "

20.5 d

u'0"'o meansinacolumnwithnocommonsuperscriptdiffersignificantly(0.0.01)

o CI:r nr:zusr fior orrir ido uriufi.: fi:s u rL'r nlnlrrfrrLin:srlu1ulfl:teuru o? tLonei't{ ril.t

(raute s) Iouriru6'ouriuff.rfirfrru'ru "l iu urnv 9o i14 fiuJoil6uoi'zusl6o rrnvrrJaitfiuoi

nr:finoon rfJu 37.50 rra; 46.66 uloir6uqi; 34.37 tLnv 36.36 rrjoir6uoi'qnruairqiu d.t

u,'Lrj usr n sjr.r riuyn{ fl fr 6 (p>0. 0 5 )

KKU. Vet. J. Vol. 14 No. I 5'n KKU Veterinory Annuol Conference 2004, 5'n KVAC 2004 31

Table 3 Fertility and hatchability of

indigenous on the storage of

eggs from layers mated with frozen semen of Thai

24 hr. and 3 months.

Dutation ol stolage frozen semen Fertility, % (fertile eggs/total) Hatchability, % (hatched/ fertile eggs)

24 hr.

3 months

s7.50 ( 15r40)

34.37 (11 /32)

46.67 (7 /15)

36.36 (4/1 1)

^ ?' X , * u,X a u t tu a d Y X ^ 3 X , d An1:?tnau1tr0ttrufl.t LnililLeJ0.l L?1u lltj?']?1.{T9ltn1Jil1t5afl''lfiTLJilna1llttattTltlj,t?1

rlt , - ri d d Z X ^ , u u , u -Y a" io ,o[14tj'.]vfrufl0.: [nilu51^rut3.r0,: fl']TT9t1*1[T000a8nu [e.Jtnu 2 xu 4v lourtt0nn:Jrill9lTtLil3

c

ai - & q d q - Anilnruo?rflo o.ru1{tun1:io'lufufi 1 rrnr 2 avfl']rJ1rnzu6nuflur.f'r16ouriurfit'[si's-O ld'mzoi'rzc9L 6l j a ^ , o6l - i ^ . ra?u LulturTrnlT:glnoa0nuLu'rult 3 rrn; 4 ilTil'lo:1jaJu'tLT0nOurrflruR.lnlTrooQtlnon.:

a?:nuolr-nrliotriorir.r#ou z iu 6ri3rusirrfiunr:lniltuerfiqiat:l nilnlilro{flrfi'oriounr:9qA dr Y 3 ' d 66t v 5l a a a ei j U ,s e6t - a j

r{notiluu'ttT0rrTtrl,rnLilildLuyt'ru0.tt9tulnunnl?n0n1TLofl0u?]truuflT.1L!11,114u1tu'tuioyt 1

uav 2 Lafiu 64.75 rr0v 76.55 rrjoir6uri'drrfjutrjnrruilloril'rurlo.rnrrn-qrfiond'lrairlr.rhqt#a' Y & , < . a Y X Au, a q ud A & i

ufludrrfiauriufir (no.:2ailrfiuil, 2537) eiruurrfioff'loi'ornnr:3o"lur-u3off 3 ufly 4 riuoyo'rt d A , a L:" 'nirtnr*'n unrlfrorrliu{Ju'r1* 24 d'rll.: l:rn6'irnr:rer6oufirr:-r]Jar.Jvlilli'r.i14rirlo.:oq6no

i a oiaao -i rn '- v I A A A -fl1n{ n{tnn00fla?1}JT'l9tl|fl31.1t[ljlJgtT.] 11J1J1.1fiU']1JT3il1ilflTJ141.{.t {,lfrAOq}d0in:"J:'tti.l'1UlJ0.1I

Lake and Stewart (1977) rruvrxnrlunr:rffrinr*rri'rt6'otu'[u1o:rqur%nlilrl.{ z+ "fi'r1eL.r t . z^ 4 vl ,a A A U r r v a A vttfl3 3 tol0u L8Jilzufla009lT1nlTnl:tnna1-[?1[[1_JU t:J1l.11{UlnlUfifl{n1:nyd'tu {.tfiooft6is{nu

rvriuvri (2s42)finnirtjrrirrfi'ouriufirsril1rnzuflsJ6oun;td'qntoiriuRrtJ rfio.rornlunnmyruriqaYoqedavletvl,Aa,

tt1j.l1.[1* A;TUO1J1J1Un1:[SJtt?tlj0dfrlt?0{0fiQ 111tUfl1tJ1TnLnlTnBltqulU[OUlriilgiAtffUqtan1:q9dvxo',<Aa-1 aAqvt(dd

tJRilqlq ttutoil?nill,["lrT0[ttttll.1?1rn!u11* 24 rxLil.: tLdv 3 t9t0u o.:Luzunt:lo5tfluci nlTt{dil

fiounrnr:finoon'lrjLranqirrnluvn.:afi6 (p>0.05) (37.50 uily 46.60 uJoirfiuai', 34.37 Lrny

36.36 r:lofr6uci'orer6f1o-1r) drffqr:rnr:zumru6ooriluinruqi'idurn-rfifinr::rr.nu1{ (o6er-nd

t[43flily, 2545: Buss, 1993; Seigneurin and Blesblois, 1995)

g)

uoldu0t[1{v

q iuv a X^, - &I 0ilnr tfl 01 nnl:fl nvrurliulj 0 xJ n tL 0Jailil{

'iQa: 1l ttlj L1 11 rlt rJ'l ; fl N 11.11 U't il n 1 : zu n n n]: tn ! Tn u't!

zu mru rfi u rull{rfforl:;RrBnrn f,J fler ei01ils9

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ilr. 1 2(1 ): 51 -62.

Birkett, J.R. 1981. Preservation of the fertilizing capacity of cock semen incubated ln Vitro.

at 40'C. Poultry Science. OO(S-O): 1075-1078.

Buss, E.G. 1993. Cryopreservation of rooster sperm. Poultry Science. 72:944-954

Calvert, J.A. 1978. Artificial lnsemination in Poultry. Bulletin 213. Ministry of Agriculture,

Fisheries and Food, London, England.

Graham, E.F., Schmehl, M.L. and Deyo, R.C.M. 1984. Cryopreservation and fertility of fish,

poultry and mamalian spermatozoa. ln: Proceedings, X Technical Conference of

Artificial lnsemination and Reproduction. National Association of Animal Breeders,

Columbia. MO. pp. 4-29

Lake, P.E. 'l 986. The history and future of the cryopreservation of avian germplasm. Poultry

Science. 65: 1 -'l 5.

KKU. Vel. J. Vol. 14 No. I 5'n KKU Veterinory Annuol Conference 2004, 5'n KVAC 2004 33

Lake, P.E. 1977. Male reproduction physiology and the collection of semen: Artificial

lnsemination in Poultry. London. 5 p.

Lake, P.E. and Stewart, J.M., 1977. Preservation of Fowl Semen in Liquid Nitrogen-an

lmproved Method. Agricultural Research Counel's Poultry Research Centre, King's

Building 5, West Mains Rood Edinburgh, EHg EHS, Scotland.

SAS. 1997" The SAS system for windows version 6.'12. SAS lnstitute lnc., Cary, NC, USA.

Schramm, G.P. 1976. Action o{ cryoprotectives glycerin, ethylene glyrerol and dimethylsulfoxide

on fertilization potential of cock semen. Mh Vet Med. 31:77O-773"

Seigneurin, F. and Blesblois, E. 1 995. Some improvements for the technique of freezing chicken

semen. ln: Proceedings: First lnternational Symposium on the Artificial lnsemiantion of

Poultry. M.R. Bakst and G.J. Wishart, (eds.). Poultry Science Association. Savoy, lL,

USA. pp. 287.(ab)

Sexton, T.J. 1977. A new poultry semen extender, Effect of extender on the fertility of chicken

semen. Poultry Science. 56(10): 1443-1446.

Sexton, T.J. 1978. A new poultry semen extender 3. Effect of storage condition on the fertilizing

capacity of chicken semen stored at 5'C. Poultry Science. 57:285-289.

Van, A.V. and Leenstra, F.R. 1995. Fertility rate of daily collected and cryopreserved fowl se-

men. Poultry Science. Z+( t -a): 1 36- 1 40.

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