SanPrep DNA SanPrep柱式DNA胶回收试剂盒

— V7.0 (6.0 w w w . s a n g o n . c o m
DNA DNA PCR
SanPrep SanPrep SanPrep
Sangon Biotech (Shanghai) Co., Ltd.
: 4 0 0 - 8 2 1 - 0 2 6 8 : 698
: w w w .sangon.com
SanPrep DNA
SanPrep DNA
SanPrep PCR PCR
PCR
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5 3 2

RNase A Buffer P1 2-8 °C 2-8 °C
Buffer P3 Buffer DW1
Buffer P1 RNase A
Wash Solution
Buffer P2 P3
SanPrep DNA DNA DNA V isualLyse 6 mL OD600 = 2.0 35 μg DNA / 20 OD260 / OD280 1.8-1.9 OD260 / OD230 2.0DNA PCR PCR
1.
2.
3.
5.
6.
7.
8.
4.

1.5-5 mL 8,000 X g 2
250 μl Buffer P1
250 μl Buffer P2 5-10 2-4
350 μl Buffer P3 5-10
12,000 X g 5-10 8,000 X g 30
500 μl Buffer DW19,000 X g 30
500 μl Wash Solution9,000 X g 30
9. 8
10. 9,000 X g 1
11. 1.5 mL 50-100 μl Elution Buffer 1 1 DNA

*
*

SanPrepDNA
50 14 mL 14 mL 20 mL 25 mL 12 mL 5 mL 210 μl 60 μl 1
100 28 mL 28 mL 40 mL 50 mL 24 mL 10 mL 420 μl 120 μl 1
+ Buffer P1 Buffer P2 Buffer P3 Buffer DW1 Wash Solut ion Elut ion Buffer RNase A VisualLyse
43 400-821-0268 www.sangon.com 698 [email protected]

1.
2.
3.
4.
5.
7.
8.
9.
≥12,000 X g 1.5 mL
RNase A Buffer P1 2-8 °C 6
Wash Solut ion 80%
Buffer P2 Buffer P3 37 °C
1/ 4
Elut ion Buffer 2.5 mM Tris-HClpH 8.5 TE pH > 7.0
Elu t ion Buffer 60 °C
> 8 kb Elu t ion Buffer 37-60 °C 2
40 μl DNA
Buffer P1 RNase A

V isualLyse 250 μl P1 1 μl V isualLyse
V i s u a l L y s e Buffer P1
5 mL
5 mL 20 μg DNA
5

V isualLyse

Buffer P3 V isualLyse 2 RNA
Wash Solut ion
DNA
End A+ BL21HB101JM End A- DH5αTOP10
350 μl Buffer P3 5-10
37 °C 12-16 h
250 μl Buffer P1
250 μl Buffer P2 5-10 2-4 min
1.5-5 mL 8,000 X g 2 min
8
10.9,000 X g 1 min
11. 50-100 μl Elut ion Buffer 1-2 min9,000 X g 1 min DNA -20 °C
500 μl Buffer DW19,000 X g 30 sec
6. ≥ 12,000 X g 5-10 min 9,000 X g 30 sec
500 μl Wash Solut ion9,000 X g 30 sec
1.
3. A.

Buffer P1P2 P3 Buffer P2 P3 37°C SOC SOB 37-60°C

pH pH > 7.0 pH 1 M pH

1 8 kb DNA Buffer P2
DNA
Buffer P1P2 P3 RNARNase A Buffer P3 5-10 Buffer P1 6 Buffer P1 100 μg / mL RNase A DNA Buffer P2 DNA Buffer P2 DNA 16 BL21 HB101 End A+ DNA DNA DW1
SanPrep 50 μl 85% 40 μl 100 μl

20
18
16
14
12
10
8
SanPrepDNA

Buffer B2 50 mL 100 mL Wash Solut ion 12 mL 24 mL Elu t ion Buffe r 5 mL 10 mL 1 1
Buffer B2

15 15 µl
TAE TBE 100 bp - 10 kb DNA 80% DNA DNA

( ) < 500 bp 1 / 3 Buffer B2
8,000 X g 30
6
9,000 X g 1
1.5 mL 15-40 μl Elution Buffer 1 1 DNA
1.
DNA

A.
B.
C.
10.
≥12,000 X g 1.5 mL
Wash Solution 80%
Buffer B2 37 °C
50 °C
DNA
≤ 1% 100 mg 300 µl Buffer B2 1%≤ 1.5% 100 mg 400 µl Buffer B2 1.5%≤ 2% 100 mg 500 µl Buffer B2 2% 100 mg 600 µl Buffer B2
Elut ion Buffer 2.5 mM Tris-HClpH 8.5 TE pH > 7.0 Elut ion Buffer 60 °C 15 μl
DNA
Wash Solut ion
400 mg DNA DNA
300 μl Buffer B29,000 X g 30 sec
DNA DNA 1.5 mL
100 mg 100 mg 100 mg 300-600 µl Buffer B2
< 500 bp Buffer B2 1/3 ≥ 500 bp 5 8,000 X g 30 sec
50°C 5-10 min
15-40 μl Elut ion Buffer 1-2 min9,000 X g 1 min DNA -20 °C
7 9,000 X g 1 min
500 μl Wash Solution9,000 X g 30 sec
Buffer B2 DNA
4 kb

pH pH > 7.0pH
1 60°C
2-5
Wash Solut ion 500 µl
4 °C -20 °C
DNA TE

1.
8,000 X g 30
PCR 5 Buffer B3
4
9,000 X g 1
1.5 mL 15-40 μl Elution Buffer 1 1 DNA
SanPrepPCR
+
E l u t io n B u f f e r Wash Solut ion Buffer B3

1.
2.
3.
Buffer B3

PCR DNA dNTP DNA
50 24 mL 12 mL 5 mL 1
100 48 mL 24 mL 10 mL 1

1.
2.
3.
4.
5.
6.
≥12,000 X g 1.5 mL
Wash Solut ion 80%
Buffer B3 20%
Buffer B3 37

750 µl 750 µl DNA
Elut ion Buf fer 2.5 mM Tris-HClpH 8.5 TE pH > 7.0
Elut ion Buf fer 60 °C
> 4 kb 37-60 °C 2
15 μl

TE 100 µl 500 µl Buffer B3
15-40 μl Elut ion Buf fer 1-2 min9,000 X g 1 min DNA -20 °C
PCR 1.5 mL 5 Buffer B3
8,000 X g 30 sec
3
9,000 X g 1 min
500 μl Wash Solut ion9,000 X g 30 sec
1. PCR
B.
C.
D.
E.
F.
G.
2.
A.
B.
C.
A.
D.
pH pH > 7.0pH
60
Buffer B3 DNA
4 kb

2-5
Wash Solut ion 500 µl
PCR T .
E. PCR 72 5-10 PCR A
PCR A PCR T
2-5

SanPrep DNA DNA DNA pH RNA DNA
Buffer P1:P2 :P3 = 5:5:7 Buffer P2 NaOH CO2
SanPrep DNA 3 DNA 40 μg
750 μl 750 μl 750 μl 600 μl 600 μl
VisualLyse VisualLyse 250 250 μl Buffer P1 1 μl VisualLyse 1:250 Buffer P1 VisualLyse Buffer P2 Buffer P3 VisualLyse
VisualLyse VisualLyse VisualLyse
DNA DNA BL21HB101JM End A+ DNA Buffer DW1 DH5 αTOP10 End A- Buffer DW1 DNA
37°C 12-16 mL 3-4 × 109 DNA RNA DNA
SanPrep DNA LB
ColE1 pMB1 pUC pBluescript 300-500 p15A pSC101 pBR322 pSC101 20

7.
6.
8.
9.
15-30
( rpm) ( RCF, X g )
PCR pH DNA DNA DNA PCR
SanPrep DNA PCR SanPrep DNA
PCR SanPrep PCR 15 μl
PCR PCR 100 bp 10 kb 10 kb 50 bp PCR PCR PCR dNTP 50-100 bp SanPrep
DNA SanPrep DNA 50 μl 85% 50 μl 100 μl 40 μl
SanPrep DNA PCR 900 Wash Solut ion
B641718
A. 6 B. 11 37-60 °C C.

19 400-821-0268 www.sangon.com 698 [email protected] 18
Note

SanPrep DNA SanPrep柱式DNA胶回收试剂盒

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