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Nederlandse norm NEN-EN-ISO 6498 (en) Animal feeding stuffs - Guidelines for sample preparation (ISO 6498:2012,IDT) Vervangt NEN-EN-ISO 6498:2009 Ontw.; NEN-ISO 6498:1998 ICS 65.120 juni 2012 Voorbeeld Preview Dit document is een voorbeeld van NEN / This document is a preview by NEN Dit document mag slechts op een stand-alone PC worden geinstalleerd. Gebruik op een netwerk is alleen. toestaan als een aanvullende licentieovereenkomst voor netwerkgebruik met NEN is afgesloten. This document may only be used on a stand-alone PC. Use in a network is only permitted when a supplementary license agreement for us in a network with NEN has been concluded.

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Page 1: Nederlandse norm NEN-EN-ISO 6498 (en) Dit document mag … · 2020-05-26 · Nederlandse norm NEN-EN-ISO 6498 (en) Animal feeding stuffs - Guidelines for sample preparation (ISO 6498:2012,IDT)

Nederlandse norm

NEN-EN-ISO 6498 (en)

Animal feeding stuffs - Guidelines for sample preparation (ISO 6498:2012,IDT)

Vervangt NEN-EN-ISO 6498:2009 Ontw.; NEN-ISO 6498:1998

ICS 65.120

juni 2012

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NEN-EN-ISO 6498

Als Nederlandse norm is aanvaard: - EN ISO 6498:2012,IDT - ISO 6498:2012,IDT

Normcommissie 370010 "Diervoeder"

THIS PUBLICATION IS COPYRIGHT PROTECTED

DEZE PUBLICATIE IS AUTEURSRECHTELIJK BESCHERMD

Apart from exceptions provided by the law, nothing from this publication may be duplicated and/or published by means of photocopy, microfilm, storage in computer files or otherwise, which also applies to full or partial processing, without the written consent of the Netherlands Standardization Institute. The Netherlands Standardization Institute shall, with the exclusion of any other beneficiary, collect payments owed by third parties for duplication and/or act in and out of law, where this authority is not transferred or falls by right to the Reproduction Rights Foundation.

Although the utmost care has been taken with this publication, errors and omissions cannot be entirely excluded. The Netherlands Standardization Institute and/or the members of the committees therefore accept no liability, not even for direct or indirect damage, occurring due to or in relation with the application of publications issued by the Netherlands Standardization Institute.

Auteursrecht voorbehouden. Behoudens uitzondering door de wet gesteld mag zonder schriftelijke toestemming van het Nederlands Normalisatie-instituut niets uit deze uitgave worden verveelvoudigd en/of openbaar gemaakt door middel van fotokopie, microfilm, opslag in computerbestanden of anderszins, hetgeen ook van toepassing is op gehele of gedeeltelijke bewerking. Het Nederlands Normalisatie-instituut is met uitsluiting van ieder ander gerechtigd de door derden verschuldigde vergoedingen voor verveelvoudiging te innen en/of daartoe in en buiten rechte op te treden, voor zover deze bevoegdheid niet is overgedragen c.q. rechtens toekomt aan de Stichting Reprorecht.

Hoewel bij deze uitgave de uiterste zorg is nagestreefd, kunnen fouten en onvolledigheden niet geheel worden uitgesloten. Het Nederlands Normalisatie-instituut en/of de leden van de commissies aanvaarden derhalve geen enkele aansprakelijkheid, ook niet voor directe of indirecte schade, ontstaan door of verband houdend met toepassing van door het Nederlands Normalisatie-instituut gepubliceerde uitgaven.

©2012 Nederlands Normalisatie-instituut Postbus 5059, 2600 GB Delft Telefoon (015) 2 690 390, Fax (015) 2 690 190

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EUROPEAN STANDARD

NORME EUROPÉENNE

EUROPÄISCHE NORM

EN ISO 6498

June 2012

ICS 65.120

English Version

Animal feeding stuffs - Guidelines for sample preparation (ISO 6498:2012)

Aliments des animaux - Lignes directrices pour la préparation des échantillons (ISO 6498:2012)

Futtermittel - Leitfaden für die Probenvorbereitung (ISO 6498:2012)

This European Standard was approved by CEN on 31 May 2012. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.

EUROPEAN COMMITTEE FOR STANDARDIZATION C O M I T É E U R O P É E N D E N O R M A LI S A T I O N EUR OP ÄIS C HES KOM ITEE FÜR NOR M UNG

Management Centre: Avenue Marnix 17, B-1000 Brussels

© 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members.

Ref. No. EN ISO 6498:2012: E

NEN-EN-ISO 6498:2012

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EN ISO 6498:2012 (E)

2

Contents Page

Foreword ..............................................................................................................................................................3

NEN-EN-ISO 6498:2012

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EN ISO 6498:2012 (E)

3

Foreword

This document (EN ISO 6498:2012) has been prepared by Technical Committee CEN/TC 327 “Animal feeding stuffs - Methods of sampling and analysis", the secretariat of which is held by NEN, in collaboration with Technical Committee ISO/TC 34 "Food products".

This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2012, and conflicting national standards shall be withdrawn at the latest by December 2012.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.

According to the CEN/CENELEC Internal Regulations, the national standards organisations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.

NEN-EN-ISO 6498:2012

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© ISO 2012

Animal feeding stuffs — Guidelines for sample preparationAliments des animaux — Lignes directrices pour la préparation des échantillons

INTERNATIONAL STANDARD

ISO6498

Third edition2012-06-01

Reference numberISO 6498:2012(E)

NEN-EN-ISO 6498:2012

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ISO 6498:2012(E)

ii © ISO 2012 – All rights reserved

COPYRIGHT PROTECTED DOCUMENT

© ISO 2012All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO’s member body in the country of the requester.

ISO copyright officeCase postale 56 • CH-1211 Geneva 20Tel. + 41 22 749 01 11Fax + 41 22 749 09 47E-mail [email protected] www.iso.org

Published in Switzerland

NEN-EN-ISO 6498:2012

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ISO 6498:2012(E)

© ISO 2012 – All rights reserved iii

Contents Page

Foreword ............................................................................................................................................................................ iv

1 Scope ...................................................................................................................................................................... 1

2 Termsanddefinitions ......................................................................................................................................... 12.1 Definitionsconcerning“sample” .................................................................................................................... 12.2 Definitionsconcerning“parameters” ............................................................................................................ 22.3 Examples of animal feeding stuffs characteristics .................................................................................... 32.4 Definitionsconcerning“samplepreparationprocedure” ........................................................................ 5

3 Principle ................................................................................................................................................................. 6

4 Consideration of sample preparation errors ............................................................................................... 74.1 Subsampling and other errors ......................................................................................................................... 74.2 Minimum mass ..................................................................................................................................................... 84.3 Errors associated with division techniques ................................................................................................. 9

5 Safety precautions ............................................................................................................................................10

6 Apparatus ............................................................................................................................................................10

7 Procedure ............................................................................................................................................................127.1 General .................................................................................................................................................................127.2 Sample check .....................................................................................................................................................127.3 Mass reduction ...................................................................................................................................................147.4 Particle size reduction .....................................................................................................................................177.5 Partial drying ......................................................................................................................................................207.6 Coarse grinding .................................................................................................................................................227.7 Special sample preparation procedures .....................................................................................................227.8 Storage .................................................................................................................................................................22

8 Performance tests (quality control) ..............................................................................................................228.1 General .................................................................................................................................................................228.2 Performance test for mass reduction (division) .......................................................................................238.3 Performance test for particle size reduction (grinding) .......................................................................... 248.4 Performance test for mixing ...........................................................................................................................25

9 Categoriesoffeeds—Specialremarksandflowcharts ....................................................................... 259.1 General .................................................................................................................................................................259.2 Birdseed ...............................................................................................................................................................269.3 Whole cottonseed .............................................................................................................................................279.4 Mineral mix ..........................................................................................................................................................299.5 Dry feeds ..............................................................................................................................................................299.6 Forages including silage, hay, haylage, TMR and byproducts ............................................................. 309.7 Oilseeds and high-fat feeds ............................................................................................................................329.8 Large block and molasses block feeds .......................................................................................................339.9 Liquid feeds ........................................................................................................................................................359.10 Canned pet food ................................................................................................................................................359.11 Semi-moist pet food and dog chews............................................................................................................369.12 Premixtures .........................................................................................................................................................379.13 Range and alfalfa hay pellets .........................................................................................................................389.14 Texturized and sticky feed ..............................................................................................................................399.15 Aquatic feeds ......................................................................................................................................................40

Annex A (informative) Calculations, examples and tables for minimum mass ............................................... 42

Bibliography .....................................................................................................................................................................46

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ISO 6498:2012(E)

Foreword

ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.

International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.

The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.

Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights.

ISO 6498 was prepared by the European Committee for Standardization (CEN) Technical Committee TC 327, Animal feeding stuffs — Methods of sampling and analysis, in collaboration with ISO Technical Committee TC 34, Food products, Subcommittee SC 10, Animal feeding stuffs, in accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).

This third edition cancels and replaces the second edition (ISO 6498:1998), which has been technically revised.

iv © ISO 2012 – All rights reserved

NEN-EN-ISO 6498:2012

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INTERNATIONAL STANDARD ISO 6498:2012(E)

Animal feeding stuffs — Guidelines for sample preparation

1 Scope

This International Standard specifies guidelines for the preparation of test samples from laboratory samples of animal feeding stuffs, including pet foods.

NOTE 1 The guidelines mostly derive from those developed by AAFCO (see Reference [7]).

The guidelines are overruled by special instructions and regulations for sample preparation demanded by specific analysis methods.

NOTE 2 Such analysis methods are developed by ISO and CEN.

NOTE 3 This International Standard does not include special guidelines for sample preparation for microbiological analysis of microorganisms like yeasts, bacteria and moulds. Nonetheless, for microorganisms which are used as feed additives (probiotics), some important aspects of sample preparation are addressed.

2 Termsanddefinitions

For the purposes of this document, the following terms and definitions apply.

2.1 Definitionsconcerning“sample”

2.1.1lotquantity of material that is assumed to be of the same production process and represented by specified sampling rules

NOTE For the purposes of this International Standard, the rules are those of Commission Regulation (EC) No. 152/2009.[3]

2.1.2laboratory samplesample as prepared (from the lot) for sending to the laboratory and intended for inspection or testing

2.1.3test samplesubsample or sample prepared from the laboratory sample and from which test portions will be taken

2.1.4test portionquantity of material drawn from the test sample (or from the laboratory sample if both are the same)

2.1.5reserve samplematerial left over from the laboratory sample when divided or subsampled test samples have been taken and on which no further particle size reduction is done

NOTE If, for example, mycotoxin or genetically modified organism analyses are done on the whole laboratory sample, then the reserve sample is also reduced to the corresponding particle sizes. The reserve sample should be stored under conditions maintaining integrity.

© ISO 2012 – All rights reserved 1

NEN-EN-ISO 6498:2012

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ISO 6498:2012(E)

2.2 Definitionsconcerning“parameters”

2.2.1parameteranalyte or constituent or microorganism for which the feeding stuff is to be analysed by microscopic, microbiological, biological or chemical procedures

2.2.1.1stable parameteranalyte or constituent or microorganism which does not degrade during sample preparation on common handling or storage at room temperatures of 20 °C to 25 °C

2.2.1.2unstable parameteranalyte or constituent or microorganism which degrades during sample preparation on common handling or storage at room temperatures of 20 °C to 25 °C because they are volatile, degradable, or sensitive to temperature, light, enzymatic degradation or chemical oxidation

NOTE Stability of parameters in this context refers only to the influence of sample preparation, such as intensive grinding, and not to a minimum shelf-life specified by producers or on the label, e.g. for a feed (additive).

Table1—Classification(ingeneral)ofstableorunstableparameters and reasons for degradation with a view to sample preparation

Origin Stable parameters Unstable parameters Reason(s) for degradation/change

Nutrients

(Crude) protein, fat, ash, fibre Moisture Temperature (volatile)

Starch, sugar, lactose Ammonia Temperature (volatile)

Gas production and enzyme-soluble organic substance production in in vitro tests

Organic acids (e.g. lactic acid, acetic acid, butyric acid, fumaric acid, formic acid)

Temperature (volatile)

Minerals (e.g. Ca, P, Mg, Na, K, Cl) Unsaturated fatty acids

Air oxidation (can result in production of short-chain fatty acids)

Feed additives

Trace elements (e.g. Cu, Zn, Mn, Fe, Se, Co)

Vitamins (e.g. vitamin A, C, D, E)

Temperature, ultraviolet (UV) light, air oxidation (sensitive)

Amino acids (e.g. lysine, methionine, tryptophan)

1,2-Propanediol, ethylene glycol Temperature (volatile)

Enzymes (e.g. phytases, non-starch polysaccharide enyzmes)

Microorganisms like probiotics (e.g. Saccharomyces cerevisiae, Enterococcus faecium)

Temperature (freezing), pressure (sensitive to grinding); moisture/dryness (influences growth of microorganisms)

Undesirable substances

Heavy metals (e.g. As, Pb, Cd, Hg)

Mycotoxins (e.g. aflatoxin B1, deoxynivalenol, fumonisins, ochratoxin A, T-2 toxin, HT-2 toxin, zearalenone, ergot alkaloids)

Mould growth and change of mycotoxins possible at room temperature; UV light (sensitive – aflatoxin B1)

Dioxins and polychlorinated biphenyls (PCBs) with similar effects to dioxins

Drugs, antibiotics, pesticides Temperature (sensitive)

Hydrocyanic acid Temperature (volatile)

Banned substances Proteins of animal origin Banned drugs, banned

antibiotics Temperature (sensitive)

(Other) Microorganisms Yeasts, bacteria, moulds

Temperature (sensitive), dryness, influx of oxygen (anaerobiosis)

2 © ISO 2012 – All rights reserved

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