LupiBreed - Fundación PROINPA · 2019. 4. 1. · Tanjil, Uniwhite, Unicrop, Lila Baer -> high...

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1 LupiBreed Improving narrow-leafed lupin Novel genetic resources for higher yield and yield stability Helge Flüß, Kristin Fischer, Steffen Roux, Regine Dieterich, Hans-Ulrich Jürgens and Brigitte Ruge-Wehling

Transcript of LupiBreed - Fundación PROINPA · 2019. 4. 1. · Tanjil, Uniwhite, Unicrop, Lila Baer -> high...

Page 1: LupiBreed - Fundación PROINPA · 2019. 4. 1. · Tanjil, Uniwhite, Unicrop, Lila Baer -> high resistance 22 early M-lines A + 10 breeding lines (22 late M-lines B + 10 breeding lines)

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LupiBreed

Improving narrow-leafed lupin – Novel genetic resources for

higher yield and yield stability

Helge Flüß, Kristin Fischer, Steffen Roux, Regine Dieterich, Hans-Ulrich Jürgens

and Brigitte Ruge-Wehling

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22ILC 2019 Cochabamba, Bolivia

LupiBreed – Consortium (2015-2018)

Brigitte Ruge-Wehling

Kristin Fischer

Steffen Roux

Peter Wehling

Gisela Jansen

Hans-Ulrich Jürgens

Regine Dieterich

Anna Beyer

Ulrike Lohwasser

Matthias Kotter

Herwart Böhm

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Part I

Improving yield…

– Identification of high-yield prebreeding lines based on a mutant population by

performance testing in different locations over 3 years (grain yield, protein

content, alkaloid content)

Improving yield stability…

– Early shattering resistance: field testing and molecular markers

– Anthracnose resistance: greenhouse and field testing, genetic mapping of

resistance loci LanrBo and Lanr1

Part II (Anne Zaar, JKI Institute for Resistance Research and Stress Tolerance)

Analysis of the range of variation for protein and alkaloid content in plant

genetic resources and novel mutant collection of narrow-leafed lupin

Aims of the consortium

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Improving yield

Increase genetic variation – EMS population

Field testing yield performance

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M0

EMS-treatment

M1 M2 M3 M4

Selection new

growth types

Propagation of

new growth types

selfing

Selection homogenous

growth types

selfing selfing

seed plant plant plant plant

Development mutant lines

Terminal

wild type

New growth types

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‚Boruta‘

‚Probor‘

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Material

cv. ‘Boruta‘, ‘Boregine‘, ‘Probor‘

22 early M-lines A (Donor: ‘Boruta’)

(22 late M-lines B (Donor: ‘Boruta’, ‘Probor’))

3 locationsGroß Lüsewitz JKI I (conv) und II (eco), Bocksee

SZS

3 years2015, 2016, 2017

Sowed5m2 – plots, 100 grains/m2

Traits

flowering, ripening, plant height, grain yield

(dt/ha), TKG, protein content, protein yield

(dt/ha), alkaloid content

Yield performance field testing

4.6.2016

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Means yield and protein, early M-lines, 2015 – 2017

LocationGrain yield

(dt/ha)

Protein

content (%)

Protein yield

(dt/ha)

GL konv Min. 33.1 28.8 9.2

BP40-47 Max. 51.1 33.0 15.2

Mean 42.5 30.2 12.5

Boruta 43.7 30.6 13.1

Probor 52.4 31.9 16.3

Boregine 57.1 28.6 15.9

GL öko Min. 20.5 24.8 5.5

BP40-47 Max. 36.4 30.5 9.8

Mean 29.6 26.8 7.8

Boruta 29.6 25.7 7.4

Probor 35.1 29.8 10.2

Boregine 41.5 27.8 11.2

Bocksee Min. 10.3 24.6 2.4

BP18-20 Max. 18.7 28.5 7.6

Mean 15.1 26.4 4.1

Boruta 15.4 26.2 3.9

Probor 17.6 28.5 5.2

Boregine 18.2 26.5 6.0

gesamt Min. 21.4 26.1 5.8

Max. 35.4 29.3 10.0

Mean 29.1 27.6 8.0

Boruta 29.6 27.5 8.2

Probor 35.0 30.0 10.5

Boregine 38.9 27.6 10.7

Good soil compostition,

some M-lines better than

wild type

No herbicides (competition)

water accessability

plant diseases, -pests

Low soil quality, water

accessability

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M-line Grain yield (dt/ha)

A4Bor 35.4 *

A15Bor 33.8 *

A3Bor 33.3 *

A18Bor 32.9 *

A5Bor 32.6 *

A19Bor 32.2

A17Bor 31.3

A12Bor 30.9

A7Bor 30.5

A9Bor 29.2

A16Bor 29.1

A14Bor 29.1

A10Bor 28.4

Boruta 29.5

Probor 35.0

Boregine 38.9

M-lineProtein content

(%)

A9Bor 29.3 *

A4Bor 28.5 *

A11Bor 28.5 *

A17Bor 28.3 *

A5Bor 28.3 *

A13Bor 28.2

A8Bor 28.1

A3Bor 28.1

A18Bor 28.0

A15Bor 27.9

A22Bor 27.6

A12Bor 27.5

A19Bor 27.5

Boruta 27.5

Probor 30.0

Boregine 27.6

M-lineAlkaloid content

(%)

A8Bor 0.010 *

A20Bor 0.011 *

A2Bor 0.014 *

A13Bor 0.015 *

A14Bor 0.015 *

A1Bor 0.016 *

A6Bor 0.018 *

A15Bor 0.018 *

A16Bor 0.018 *

A19Bor 0.019 *

A9Bor 0.020 *

A12Bor 0.020 *

A22Bor 0.021 *

Boruta 0.026

Probor 0.027

Boregine 0.039

Yield performance early M-lines, A

9 environments (3 locations, 2015-2017)

Variant analysis LSD-Test, =0.05A Bor Mutant line based on cv ‚Boruta‘ Comparison to wild type ‚Boruta‘

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Early shattering resistance

Increasing yield stability

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Material

VB Antaniai -> strongly shattering

Tanjil, Uniwhite, Unicrop, Lila Baer -> high

resistance

22 early M-lines A + 10 breeding lines

(22 late M-lines B + 10 breeding lines)

2 locationsGroß Lüsewitz JKI, Bocksee SZS

3 years2015, 2016, 2017

Sowing2-row micro plots, 5 x 5 grids

Scoring1 – 9 (shattering resisant – highly shattering)

Shattering resistance field testing

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Shattering resistance, early M-lines, 2015 – 2017, A

highly shattering (9)

No shattering (1)

VB Antaniani

Tanjil

Uniwhite

Unicrop

Breeding lines

Mutant lines

Specimens

Sco

res

Boruta (1 Jahr)

Lila Baer (1 Jahr)

Kruskal –Wallis-Test

(c2 = 155.7, df = 35, p = < 0.05)

PostHoc: Dunn-Bonferroni-Test 22.03.2019 ILC 2019 Cochabamba, Bolivia 11

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Molecular characterization

Example: tardus

Marker: TaLi (tardus, Li et al., 2010)

+- + + + -- - -

Breeding lines

Mutant-lines

+ +-/ - -

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Anthracnose resistance

Increasing yield stability

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Resistance resources

Resource Resistance-

level

Inheritance Loucs LG Selection-

marker

cv. ‚Tanjil‘

(Australia)

High

(some spots on

pods)

monogenic

dominant

Lanr 1* NLL-11

(distal)

Lanr1_1

Lanr1_2

Zuchtlinie

Bo7212

(SZS)

High

(almost no

symptoms)

monogenic

dominant

LanrBo** NLL-11 BoSeq196

LanM_19

Greenhouse testing

Field testing*Yang et al. 2004

**Fischer et al. 2015

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Allelic association test

hor hor

x

her

Lanr1 LanrBo

Lanr1+ LanrBo

F1

S

her her sushor

F2-PopulationPhenotype – Resistance

0

50

100

150

200

250

300

resistant susceptible

Nu

mb

er

of

pla

nts

exp

obs

21

0

20

40

60

80

100

120

140

160

180

LanrBo +Lanr1

Lanr1 LanrBo -

Nu

mb

er

of

pla

nts

obs

exp

Genotype – Marker

15

c2 15:1= 0.66

1

9

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1

‚Tanjil‘ Bo7212

hor = homozygous resistant for Lanr1 and LanrBo

her = heterozygous resistant für Lanr1 and/or LanrBo

sus. = susceptible

N= 283

N= 283

262

151 49 62 21

c2 9:3:3:1 = 2.9

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Mapping of resistance locus

www.lupinexpress.com

ILC 2019 Cochabamba, Bolivia22.03.2019 16

BoSeq37DAFWA3348ArSeq423LaIND_096BoSeq67BoSeq62ArSeq105BoSeq41 BoSeq61BoSeq720LaIND_085

LJM7_1Lanr1_1Lanr1_2DAFWA7361AntjM1LanM_02Lup015155LanM_03

BoSeq196

LanrBoLanM_16LanM_19BoSeq1108Lup011620BoSeq83BoSeq5Lup007149

LaIND_138

DAFWA2850

2.81.28.22.10.90.21.00.10.3

14.5

0.91.21.32.25.10.62.9

17.6

0.40.91.65.23.31.44.70.9

9.2

4.5

1

Lanr1

LanrBo

~ 30 cM

6.5 Mbp

9.5 Mbp

4.1 Mbp

15.5 Mbp

Genetic map (cM) Physical map (Mbp)

NLL-11

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Sum up yield performance and stability

Selection of mutant-lines with multiple improved traits pre-

breeding

Marker assisted breedingc approaches possible for shattering and anthracnose

resistance

Mutant lines:

Significantly higher grain yields compared to donor ‚Boruta‘ (9 environments)

Significantly higher protein content compared to high yield cultivar ‚Boregine‘ positive

effect on protein yield

22.03.2019 ILC 2019 Cochabamba, Bolivia

Significantly higher grain yield compared to high yield cultivar ‚Boregine‘ in poor soil

location

High heritability breeding progress

Significantly lower alkaloid contents in early types

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Madlen Christoffer

Juliane Böttcher

Kerstin Kadzban

Birgit Schütt

ILC 2019 Cochabamba, Bolivia

Thanks to...

Nicolas Krezdorn, GenXPro

Björn Rotter, GenXPro

Matt Nelson, UWA

Lars Kamphuis, Karam Singh, CSIRO

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19ILC 2019 Cochabamba, Bolivia

Vielen Dank für Ihr Interesse!

Thank you for your attention!

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Page 20: LupiBreed - Fundación PROINPA · 2019. 4. 1. · Tanjil, Uniwhite, Unicrop, Lila Baer -> high resistance 22 early M-lines A + 10 breeding lines (22 late M-lines B + 10 breeding lines)

2020

Part I

Improving yield…

– Identification of high-yield prebreeding lines based on a mutant population by

performance testing in different locations over 3 years (grain yield, protein

content, alkaloid content)

Improving yield stability…

– Early shattering resistance: field testing and molecular markers

– Anthracnose resistance: greenhouse and field testing, genetic mapping of

resistance loci LanrBo and Lanr1

Part II (Anne Zaar, JKI Institute for Resistance Research and Stress Tolerance)

Analysis of the range of variation for protein and alkaloid content in plant

genetic resources novel mutant collection of narrow-leafed lupin

Aims of the consortium

22.03.2019 ILC 2019 Cochabamba, Bolivia 20