Effecten van fructooligosacchariden

bij zogende biggen

Dirkjan Schokker et al. ~ 21-4-2015

Animal Breeding & Genomics Centre

Acknowledgements

Animal Breeding & Genomics Centre

Ministerie van Economische Zaken

Productschap Diervoeder (PDV)

Productschap Pluimvee en Eieren (PPE)

Productschap Vee en Vlees (PVV)

Agrifirm Group

ForFarmers Hendrix

De Heus

Nutreco

VION Food Group

MSD - Animal Health

VanDrie Group

Denkavit

Voeding, Darmgezondheid, en

Immuniteit

Focus on gut development in early life

Animal Breeding & Genomics Centre

Morphological

developmentImmune system

programming

Microbiota

colonization

Functional

differentiation

Nutritional

factorsManagement

factors

Interactions between intestinal

microbiota and the immune system

Host

Microbiota Environment

GUT

Gut is the gatekeeper of health

Objective

Investigate the effect of FOS on microbiota colonization and immune

development of the intestine in relation to immune competence

in suckling piglets

Animal Breeding & Genomics Centre

Study design

Control

FOS

Day 2 14 25

8 8 8

8 8

Animal Breeding & Genomics Centre

� 2 * 5gr FOS oral gavage

� From day 2~6 diluted in 15ml water

� Due to vomiting/diarrhoea day 7~14 diluted in 6ml water

Performance data

0

1

2

3

4

5

6

7

8

9

10

0 3 7 14 21 27

Weig

ht

(in

kg

)

Time (day)

Average body weight

Control

FOS

0

50

100

150

200

250

300

350

400

Control Control FOS Control FOS Control FOS

0-3 3-7 7-14 14-21

Weig

ht

(in

gram

s)

Growth per day

Average daily gain

Effect on gut bacteria in colon

Treatment Diversity

Control.14 3.12 ± 0.06

FOS.14 3.16 ± 0.06

CompositionShannon diversity

Effect on gut bacteria in colon

Bifidogenic effect

Targeting small intestine

Small intestine Large intestine

Mowat and Agace, 2014

Bacteria composition and diversity in

jejunum

Animal Breeding & Genomics Centre

Abundance of bacteria (top 10)

Abundance of bacteria (top 10)

�No significant differences

�High variance

� Trends lower Lactobacillliand higher E.coli in piglets receiving FOS

Histology

Control

FOS

Animal Breeding & Genomics Centre

Gene expression in jejunum

Animal Breeding & Genomics Centre

Day 14 Day 25

Cell cycle

Extracellular matrix

turn-over of

epithelial cells tight junctions /

barrier function

Immune

more energy for

intestinal tissue

higher diversity

of microbiota

No higher villi (yet)

Conclusions

FOS supplementation to suckling piglets has a

bifidogenic effect in the colon on day 14 after birth

Jejunal gene expression of FOS piglets showed lower

activity of immunological genes compared to the

controls

Increased diversity in jejunum in piglets

receiving FOS

Lower activity of cell cycle related processes,

and higher activity extracellular matrix

processes showed in piglets receiving FOS

14

25

25

14

Potential indicators

for immune competence

Animal Breeding & Genomics Centre

dirkjan.schokker@wur.nl

+31 (0)317 480537

Thank you for your attention